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Activators of protein kinase C trigger cortical granule exocytosis, cortical contraction, and cleavage furrow formation in Xenopus laevis oocytes and eggs.
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Prophase I oocytes, free of follicle cells, and metaphase II eggs of the amphibian Xenopus laevis were subjected to transient treatments with the protein kinase C activators, phorbol 12-myristate 13-acetate (PMA), phorbol 12,13-didecanoate, and 1-olyeoyl-2-acetyl-sn-glycerol. In both oocytes and eggs, these treatments triggered early events of amphibian development: cortical granule exocytosis, cortical contraction, and cleavage furrow formation. Surprisingly, activation of oocytes occurred in the absence of meiotic resumption, resulting in cells with an oocytelike nucleus and interior cytoplasm, but with a zygotelike cortex. PMA-induced activation of oocytes and eggs did not require external calcium, a prerequisite for normal activation of eggs. PMA-induced activation of eggs was inhibited by retinoic acid, a known inhibitor of protein kinase C. In addition, pretreatment of eggs with retinoic acid prevented activation by mechanical stimulation and inhibited activation by calcium ionophore A23187. The results suggest that protein kinase C activation is an integral component of the Xenopus fertilization pathway.
Ashendel,
Protein kinase activity associated with a phorbol ester receptor purified from mouse brain.
1983, Pubmed
Ashendel,
Protein kinase activity associated with a phorbol ester receptor purified from mouse brain.
1983,
Pubmed Bell,
Protein kinase C activation by diacylglycerol second messengers.
1986,
Pubmed Bement,
Intracellular signals trigger ultrastructural events characteristic of meiotic maturation in oocytes of Xenopus laevis.
1989,
Pubmed
,
Xenbase Berridge,
Inositol trisphosphate and diacylglycerol as second messengers.
1984,
Pubmed Bluemink,
Cortical wound healing in the amphibian egg: an electron microscopical study.
1972,
Pubmed
,
Xenbase Blumberg,
Protein kinase C as the receptor for the phorbol ester tumor promoters: sixth Rhoads memorial award lecture.
1988,
Pubmed Busa,
An elevated free cytosolic Ca2+ wave follows fertilization in eggs of the frog, Xenopus laevis.
1985,
Pubmed
,
Xenbase Busa,
Activation of frog (Xenopus laevis) eggs by inositol trisphosphate. I. Characterization of Ca2+ release from intracellular stores.
1985,
Pubmed
,
Xenbase Campanella,
The modifications of cortical endoplasmic reticulum during in vitro maturation of Xenopus laevis oocytes and its involvement in cortical granule exocytosis.
1984,
Pubmed
,
Xenbase Campanella,
Ultrastructural observations on cortical endoplasmic reticulum and on residual cortical granules in the egg of Xenopus laevis.
1977,
Pubmed
,
Xenbase Capco,
Analysis of proteins in the peripheral and central regions of amphibian oocytes and eggs.
1988,
Pubmed
,
Xenbase Castagna,
Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters.
1982,
Pubmed Charbonneau,
The onset of activation responsiveness during maturation coincides with the formation of the cortical endoplasmic reticulum in oocytes of Xenopus laevis.
1984,
Pubmed
,
Xenbase Ciapa,
Structural modifications induced by TPA (12-O-tetradecanoyl phorbol-13-acetate) in sea urchin eggs.
1988,
Pubmed Clapper,
Inositol trisphosphate induces calcium release from nonmitochondrial stores i sea urchin egg homogenates.
1985,
Pubmed Cuthbertson,
Free Ca2+ increases in exponential phases during mouse oocyte activation.
1981,
Pubmed Di Virgilio,
Protein kinase C activation of physiological processes in human neutrophils at vanishingly small cytosolic Ca2+ levels.
,
Pubmed Doherty,
Comparison of the effects of NGF, activators of protein kinase C, and a calcium ionophore on the expression of Thy-1 and N-CAM in PC12 cell cultures.
1988,
Pubmed Eisen,
Temporal sequence and spatial distribution of early events of fertilization in single sea urchin eggs.
1984,
Pubmed Endo,
Effects of phorbol esters and a diacylglycerol on mouse eggs: inhibition of fertilization and modification of the zona pellucida.
1987,
Pubmed Gardiner,
Membrane junctions in Xenopus eggs: their distribution suggests a role in calcium regulation.
1983,
Pubmed
,
Xenbase Gilkey,
Roles of calcium and pH in activation of eggs of the medaka fish, Oryzias latipes.
1983,
Pubmed Gilkey,
A free calcium wave traverses the activating egg of the medaka, Oryzias latipes.
1978,
Pubmed Grey,
Formation and structure of the fertilization envelope in Xenopus laevis.
1974,
Pubmed
,
Xenbase Heidemann,
Aster formation in eggs of Xenopus laevis. Induction by isolated basal bodies.
1975,
Pubmed
,
Xenbase Heidemann,
Induced formation of asters and cleavage furrows in oocytes of Xenopus laevis during in vitro maturation.
1978,
Pubmed
,
Xenbase Hirota,
Hormone-induced redistribution of calcium-activated phospholipid-dependent protein kinase in pituitary gonadotrophs.
1985,
Pubmed Hollinger,
"Cleavage" and cortical granule breakdown in Rana pipiens oocytes induced by direct microinjection of calcium.
1976,
Pubmed Ito,
Immunocytochemical evidence for translocation of protein kinase C in human megakaryoblastic leukemic cells: synergistic effects of Ca2+ and activators of protein kinase C on the plasma membrane association.
1988,
Pubmed Kaibuchi,
Synergistic functions of protein phosphorylation and calcium mobilization in platelet activation.
1983,
Pubmed Kajikawa,
A possible role of protein kinase C in signal-induced lysosomal enzyme release.
1983,
Pubmed Kishimoto,
Activation of calcium and phospholipid-dependent protein kinase by diacylglycerol, its possible relation to phosphatidylinositol turnover.
1980,
Pubmed Kline,
Fertilization events induced by neurotransmitters after injection of mRNA in Xenopus eggs.
1988,
Pubmed
,
Xenbase Kubota,
Free calcium wave upon activation in Xenopus eggs.
1987,
Pubmed
,
Xenbase Laurent,
Involvement of a calcium-phospholipid-dependent protein kinase in the maturation of Xenopus laevis oocytes.
1988,
Pubmed
,
Xenbase May,
Role of intracellular calcium mobilization in the regulation of protein kinase C-mediated membrane processes.
,
Pubmed Miyazaki,
Inositol 1,4,5-trisphosphate-induced calcium release and guanine nucleotide-binding protein-mediated periodic calcium rises in golden hamster eggs.
1988,
Pubmed Miyazaki,
Temporal and spatial dynamics of the periodic increase in intracellular free calcium at fertilization of golden hamster eggs.
1986,
Pubmed Newport,
A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage.
1982,
Pubmed
,
Xenbase Nishizuka,
The role of protein kinase C in cell surface signal transduction and tumour promotion.
,
Pubmed Nuccitelli,
The wave of activation current in the egg of the medaka fish.
1987,
Pubmed Picard,
Inositol 1,4,5-triphosphate microinjection triggers activation, but not meiotic maturation in amphibian and starfish oocytes.
1985,
Pubmed
,
Xenbase Pontremoli,
Phosphorylation and proteolytic modification of specific cytoskeletal proteins in human neutrophils stimulated by phorbol 12-myristate 13-acetate.
1987,
Pubmed Sando,
Identification of high-affinity phorbol ester receptor in cytosol of EL4 thymoma cells: requirement for calcium, magnesium, and phospholipids.
1983,
Pubmed Shen,
1,2-Diacylglycerols mimic phorbol 12-myristate 13-acetate activation of the sea urchin egg.
1986,
Pubmed Steinhardt,
Intracellular calcium release at fertilization in the sea urchin egg.
1977,
Pubmed Stith,
Induction of meiotic maturation in Xenopus oocytes by 12-O-tetradecanoylphorbol 13-acetate.
1987,
Pubmed
,
Xenbase Swann,
Stimulation of the Na/H exchanger of sea urchin eggs by phorbol ester.
,
Pubmed Swann,
The part played by inositol trisphosphate and calcium in the propagation of the fertilization wave in sea urchin eggs.
1986,
Pubmed Taffet,
Retinal inhibits TPA activated, calcium-dependent, phospholipid-dependent protein kinase ("C" kinase).
1983,
Pubmed Turner,
Fertilization increases the polyphosphoinositide content of sea urchin eggs.
,
Pubmed Ubbels,
Evidence for a functional role of the cytoskeleton in determination of the dorsoventral axis in Xenopus laevis eggs.
1983,
Pubmed
,
Xenbase Verma,
Correlation of the inhibition by retinoids of tumor promoter-induced mouse epidermal ornithine decarboxylase activity and of skin tumor promotion.
1979,
Pubmed Wallace,
Protein incorporation by isolated amphibian oocytes. 3. Optimum incubation conditions.
1973,
Pubmed
,
Xenbase Wolf,
The cortical granule reaction in living eggs of the toad, Xenopus laevis.
1974,
Pubmed
,
Xenbase Wolf,
On the contents of the cortical granules from Xenopus laevis eggs.
1974,
Pubmed
,
Xenbase Wolf,
A model for intracellular translocation of protein kinase C involving synergism between Ca2+ and phorbol esters.
,
Pubmed Wolf,
The cortical response in Xenopus laevis ova.
1974,
Pubmed
,
Xenbase Yamanishi,
Synergistic functions of phorbol ester and calcium in serotonin release from human platelets.
1983,
Pubmed
