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Structure of the NGFI-A gene and detection of upstream sequences responsible for its transcriptional induction by nerve growth factor.
Changelian PS, Feng P, King TC, Milbrandt J.
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The NGFI-A gene encodes a "zinc-finger" protein that is rapidly induced by nerve growth factor (NGF) in PC12 rat pheochromocytoma cells. The complete exon/intron organization and nucleotide sequence of the rat NGFI-A gene have been determined. The gene spans 3789 nucleotides (nt) and is interrupted by a single intron at nt 588. All three zinc-finger DNA-binding domains are contiguously coded for within the 3' exon; this is in contrast to the structure described by others for the Xenopus laevis transcription factor TFIIIA gene. To analyze the transcription of this gene, we have determined the transcription start site and nucleotide sequence of the 5' flanking region. Transfection of PC12 cells with a fragment from the 5' flanking region linked to the chloramphenicol acetyltransferase (CAT) gene revealed that it contains an element which imparts an NGF-inducible phenotype to the normally silent CAT gene. Several regions with homologies to recognizable sequence elements are present in this fragment, including a TATA box at nt -27, serum response elements at nt -84, -106, -370, and -408, a cAMP-responsive element at nt -140, and a transcription factor Sp1-binding site at nt -286. These results establish the genomic structure of this mammalian multifinger protein and demonstrate that an NGF-responsive element lies upstream of the NGFI-A gene.
Chavrier,
A gene encoding a protein with zinc fingers is activated during G0/G1 transition in cultured cells.
1988, Pubmed
Chavrier,
A gene encoding a protein with zinc fingers is activated during G0/G1 transition in cultured cells.
1988,
Pubmed Chavrier,
Characterization of a mouse multigene family that encodes zinc finger structures.
1988,
Pubmed Chirgwin,
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
1979,
Pubmed Chowdhury,
A multigene family encoding several "finger" structures is present and differentially active in mammalian genomes.
1987,
Pubmed
,
Xenbase Curran,
Superinduction of c-fos by nerve growth factor in the presence of peripherally active benzodiazepines.
1985,
Pubmed Dynan,
The promoter-specific transcription factor Sp1 binds to upstream sequences in the SV40 early promoter.
1983,
Pubmed Evans,
Zinc fingers: gilt by association.
1988,
Pubmed Feinberg,
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
1983,
Pubmed Fromm,
Transcription in vivo from SV40 early promoter deletion mutants without repression by large T antigen.
1983,
Pubmed Garber,
Genomic and cDNA clones of the homeotic locus Antennapedia in Drosophila.
1983,
Pubmed Gorman,
Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
1982,
Pubmed Grunstein,
Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
1975,
Pubmed Kadonaga,
Isolation of cDNA encoding transcription factor Sp1 and functional analysis of the DNA binding domain.
1987,
Pubmed Karns,
Cloning of complementary DNA for GAP-43, a neuronal growth-related protein.
1987,
Pubmed Lau,
Expression of a set of growth-related immediate early genes in BALB/c 3T3 cells: coordinate regulation with c-fos or c-myc.
1987,
Pubmed Leonard,
A nerve growth factor-regulated messenger RNA encodes a new intermediate filament protein.
1988,
Pubmed Masiakowski,
Nerve growth factor induces the genes for two proteins related to a family of calcium-binding proteins in PC12 cells.
1988,
Pubmed Milbrandt,
Nerve growth factor induces a gene homologous to the glucocorticoid receptor gene.
1988,
Pubmed Milbrandt,
A nerve growth factor-induced gene encodes a possible transcriptional regulatory factor.
1987,
Pubmed
,
Xenbase Milbrandt,
Organization of a Chinese hamster ovary dihydrofolate reductase gene identified by phenotypic rescue.
1983,
Pubmed Milbrandt,
Nerve growth factor rapidly induces c-fos mRNA in PC12 rat pheochromocytoma cells.
1986,
Pubmed Miller,
Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes.
1985,
Pubmed
,
Xenbase Miwa,
CArG boxes in the human cardiac alpha-actin gene are core binding sites for positive trans-acting regulatory factors.
1987,
Pubmed Montminy,
Binding of a nuclear protein to the cyclic-AMP response element of the somatostatin gene.
,
Pubmed Page,
The sex-determining region of the human Y chromosome encodes a finger protein.
1987,
Pubmed Potter,
Enhancer-dependent expression of human kappa immunoglobulin genes introduced into mouse pre-B lymphocytes by electroporation.
1984,
Pubmed Rauscher,
Fos-associated protein p39 is the product of the jun proto-oncogene.
1988,
Pubmed Reed,
Rapid transfer of DNA from agarose gels to nylon membranes.
1985,
Pubmed Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed Sukhatme,
A zinc finger-encoding gene coregulated with c-fos during growth and differentiation, and after cellular depolarization.
1988,
Pubmed Treisman,
Identification of a protein-binding site that mediates transcriptional response of the c-fos gene to serum factors.
1986,
Pubmed Tso,
Structure of the gene for Xenopus transcription factor TFIIIA.
1986,
Pubmed
,
Xenbase Yankner,
The biology and mechanism of action of nerve growth factor.
1982,
Pubmed
