Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
1988 Nov 01;8521:7917-21.
Show Gene links
Show Anatomy links
Expression of a bovine rhodopsin gene in Xenopus oocytes: demonstration of light-dependent ionic currents.
Khorana HG, Knox BE, Nasi E, Swanson R, Thompson DA.
???displayArticle.abstract???
Xenopus oocytes express a gene encoding bovine rhodopsin as well as its SP6 RNA polymerase-derived transcripts and total retinal mRNA. The opsin produced is in unglycosylated (30 kDa) and two glycosylated (35 kDa and 41 kDa) forms. Incubation of the cells expressing the above proteins with 11-cis-retinal generates rhodopsin, which was purified by immunoaffinity chromatography. The purified protein shows the expected UV/visible absorption spectrum and characteristic light-dependent activation of the rod outer segment GTP binding protein. Oocytes expressing rhodopsin exhibit light-dependent ionic currents that are detected by voltage-clamp techniques.
Baehr,
Characterization of bovine rod outer segment G-protein.
1982, Pubmed
Baehr,
Characterization of bovine rod outer segment G-protein.
1982,
Pubmed Barish,
A transient calcium-dependent chloride current in the immature Xenopus oocyte.
1983,
Pubmed
,
Xenbase Bridges,
A method for preparing stable digitonin solutions for visual pigment extraction.
1977,
Pubmed Cerione,
Specificity of the functional interactions of the beta-adrenergic receptor and rhodopsin with guanine nucleotide regulatory proteins reconstituted in phospholipid vesicles.
1985,
Pubmed Chirgwin,
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
1979,
Pubmed Dascal,
The use of Xenopus oocytes for the study of ion channels.
1987,
Pubmed
,
Xenbase Dascal,
Involvement of a GTP-binding protein in mediation of serotonin and acetylcholine responses in Xenopus oocytes injected with rat brain messenger RNA.
1986,
Pubmed
,
Xenbase Dohlman,
A family of receptors coupled to guanine nucleotide regulatory proteins.
1987,
Pubmed Drummond,
The effect of capping and polyadenylation on the stability, movement and translation of synthetic messenger RNAs in Xenopus oocytes.
1985,
Pubmed
,
Xenbase Elbein,
Inhibitors of the biosynthesis and processing of N-linked oligosaccharide chains.
1987,
Pubmed Ferretti,
Total synthesis of a gene for bovine rhodopsin.
1986,
Pubmed Franke,
A single amino acid substitution in rhodopsin (lysine 248----leucine) prevents activation of transducin.
1988,
Pubmed Fuhrmann,
Novel mannosidase inhibitor blocking conversion of high mannose to complex oligosaccharides.
,
Pubmed Gilman,
G proteins: transducers of receptor-generated signals.
1987,
Pubmed Kline,
Fertilization events induced by neurotransmitters after injection of mRNA in Xenopus eggs.
1988,
Pubmed
,
Xenbase Konarska,
Recognition of cap structure in splicing in vitro of mRNA precursors.
1984,
Pubmed Kubo,
Cloning, sequencing and expression of complementary DNA encoding the muscarinic acetylcholine receptor.
,
Pubmed
,
Xenbase Kuo,
Multiple opsin mRNA species in bovine retina.
1986,
Pubmed Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed Lübbert,
cDNA cloning of a serotonin 5-HT1C receptor by electrophysiological assays of mRNA-injected Xenopus oocytes.
1987,
Pubmed
,
Xenbase Masu,
cDNA cloning of bovine substance-K receptor through oocyte expression system.
,
Pubmed
,
Xenbase Melton,
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
1984,
Pubmed Nomura,
Inositol phosphate formation and chloride current responses induced by acetylcholine and serotonin through GTP-binding proteins in Xenopus oocyte after injection of rat brain messenger RNA.
1987,
Pubmed
,
Xenbase Oprian,
Expression of a synthetic bovine rhodopsin gene in monkey kidney cells.
1987,
Pubmed Romero,
N-methyl-1-deoxynojirimycin, a novel inhibitor of glycoprotein processing, and its effect on fowl plague virus maturation.
1983,
Pubmed Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed Sippel,
Purification and characterization of adenosine triphosphate: ribonucleic acid adenyltransferase from Escherichia coli.
1973,
Pubmed Stryer,
Cyclic GMP cascade of vision.
1986,
Pubmed Takahashi,
Rat brain serotonin receptors in Xenopus oocytes are coupled by intracellular calcium to endogenous channels.
1987,
Pubmed
,
Xenbase Tarentino,
Deglycosylation of asparagine-linked glycans by peptide:N-glycosidase F.
1985,
Pubmed Tarentino,
Purification and properties of an endo-beta-N-acetylglucosaminidase from Streptomyces griseus.
1974,
Pubmed Wald,
The molecular basis of visual excitation.
1968,
Pubmed WALD,
Synthesis and bleaching of rhodopsin.
1956,
Pubmed
