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The human estrogen receptor can regulate exogenous but not endogenous vitellogenin gene promoters in a Xenopus cell line.
Seiler-Tuyns A, Mérillat AM, Haefliger DN, Wahli W.
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Transfection of a human estrogen receptor cDNA expression vector (HEO) into cultured Xenopus kidney cells confers estrogen responsiveness to the recipient cells as demonstrated by the hormone dependent expression of co-transfected Xenopus vitellogenin-CAT chimeric genes. The estrogen stimulation of these vit-CAT genes is dependent upon the presence of the vitellogenin estrogen responsive element (ERE) in their 5' flanking region. Thus, functional human estrogen receptor (hER) can be synthesized in heterologous lower vertebrate cells and can act as a trans-acting regulatory factor that is necessary, together with estradiol, for the induction of the vit-CAT constructs in these cells. In addition, vitellogenin minigenes co-transfected with the HEO expression vector also respond to hormonal stimulation. Their induction is not higher than that of the vit-CAT chimeric genes. It suggests that in the Xenopus kidney cell line B 3.2, the structural parts of the vitellogenin minigenes do not play a role in the induction process. Furthermore, no stabilizing effect of estrogen on vitellogenin mRNA is observed in these cells. In contrast to the transfected genes, the endogenous chromosomal vitellogenin genes remain silent, demonstrating that in spite of the presence of the hER and the hormone, the conditions necessary for their activation are not fulfilled.
Andres,
Persistence, methylation and expression of vitellogenin gene derivatives after injection into fertilized eggs of Xenopus laevis.
1984, Pubmed,
Xenbase
Andres,
Persistence, methylation and expression of vitellogenin gene derivatives after injection into fertilized eggs of Xenopus laevis.
1984,
Pubmed
,
Xenbase Baker,
Kinetics of estrogen induction of Xenopus laevis vitellogenin messenger RNA as measured by hybridization to complementary DNA.
1977,
Pubmed
,
Xenbase Berthois,
Phenol red in tissue culture media is a weak estrogen: implications concerning the study of estrogen-responsive cells in culture.
1986,
Pubmed Breathnach,
Plasmids for the cloning and expression of full-length double-stranded cDNAs under control of the SV40 early or late gene promoter.
1983,
Pubmed Brock,
Estrogen stabilizes vitellogenin mRNA against cytoplasmic degradation.
1983,
Pubmed
,
Xenbase Buetti,
Distinct sequence elements involved in the glucocorticoid regulation of the mouse mammary tumor virus promoter identified by linker scanning mutagenesis.
1986,
Pubmed Danesch,
Glucocorticoid induction of the rat tryptophan oxygenase gene is mediated by two widely separated glucocorticoid-responsive elements.
1987,
Pubmed Druege,
Introduction of estrogen-responsiveness into mammalian cell lines.
1986,
Pubmed
,
Xenbase Germond,
Vitellogenin B2 gene in Xenopus laevis: isolation, in vitro transcription and relation to other vitellogenin genes.
1983,
Pubmed
,
Xenbase Gorman,
Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
1982,
Pubmed Green,
Human oestrogen receptor cDNA: sequence, expression and homology to v-erb-A.
,
Pubmed Green,
Oestradiol induction of a glucocorticoid-responsive gene by a chimaeric receptor.
,
Pubmed
,
Xenbase Green,
A superfamily of potentially oncogenic hormone receptors.
,
Pubmed Klein-Hitpass,
A 13 bp palindrome is a functional estrogen responsive element and interacts specifically with estrogen receptor.
1988,
Pubmed
,
Xenbase Knowland,
Injection of partially purified estrogen receptor protein from Xenopus liver nuclei into oocytes activates the silent vitellogenin locus.
1984,
Pubmed
,
Xenbase Knowler,
The mechanism of action of oestrogens.
1985,
Pubmed Koike,
Molecular cloning and characterization of rat estrogen receptor cDNA.
1987,
Pubmed Krust,
The chicken oestrogen receptor sequence: homology with v-erbA and the human oestrogen and glucocorticoid receptors.
1986,
Pubmed Kumar,
Functional domains of the human estrogen receptor.
1987,
Pubmed Lopata,
High level transient expression of a chloramphenicol acetyl transferase gene by DEAE-dextran mediated DNA transfection coupled with a dimethyl sulfoxide or glycerol shock treatment.
1984,
Pubmed Martinez,
The estrogen-responsive element as an inducible enhancer: DNA sequence requirements and conversion to a glucocorticoid-responsive element.
1987,
Pubmed
,
Xenbase Miksicek,
Glucocorticoid responsiveness of the transcriptional enhancer of Moloney murine sarcoma virus.
1986,
Pubmed Parker,
Regulation of simian virus 40 transcription: sensitive analysis of the RNA species present early in infections by virus or viral DNA.
1979,
Pubmed Prud'homme,
Cloning of a gene expressed in human breast cancer and regulated by estrogen in MCF-7 cells.
1985,
Pubmed Ryffel,
Quantitation of vitellogenin messenger RNA in the liver of male Xenopus toads during primary and secondary stimulation by estrogen.
1977,
Pubmed
,
Xenbase Scheidereit,
Molecular model of the interaction between the glucocorticoid receptor and the regulatory elements of inducible genes.
1986,
Pubmed Seiler-Tuyns,
Cell cycle regulation of a mouse histone H4 gene requires the H4 promoter.
1987,
Pubmed Seiler-Tuyns,
Identification of estrogen-responsive DNA sequences by transient expression experiments in a human breast cancer cell line.
1986,
Pubmed
,
Xenbase ten Heggeler-Bordier,
Electron microscopic visualization of protein-DNA interactions at the estrogen responsive element and in the first intron of the Xenopus laevis vitellogenin gene.
1987,
Pubmed
,
Xenbase Theulaz,
Expression of human estrogen receptor mutants in Xenopus oocytes: correlation between transcriptional activity and ability to form protein-DNA complexes.
1988,
Pubmed
,
Xenbase Wahli,
Xenopus vitellogenin genes.
1985,
Pubmed
,
Xenbase Walker,
Sequence homologies in the region preceding the transcription initiation site of the liver estrogen-responsive vitellogenin and apo-VLDLII genes.
1984,
Pubmed
,
Xenbase Walter,
Cloning of the human estrogen receptor cDNA.
1985,
Pubmed Weiler,
The Xenopus laevis estrogen receptor: sequence homology with human and avian receptors and identification of multiple estrogen receptor messenger ribonucleic acids.
1987,
Pubmed
,
Xenbase Westley,
An estrogen receptor from Xenopus laevis liver possibly connected with vitellogenin synthesis.
1978,
Pubmed
,
Xenbase Wiskocil,
Coordinate regulation of two estrogen-dependent genes in avian liver.
1980,
Pubmed
