Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Homologous genes for mouse 4.5S hybRNA are found in all eukaryotes and their low molecular weight RNA transcripts intermolecularly hybridize with eukaryotic 18S ribosomal RNAs.
Trinh-Rohlik Q, Maxwell ES.
???displayArticle.abstract???
Previous work has reported the isolation and sequencing of a mouse low molecular weight RNA species designated 4.5S hybridizing RNA or hybRNA because of its ability to intermolecularly hybridize with mouse mRNA and 18S rRNA sequences. Using synthetic DNA oligonucleotide probes we have examined the conservation of this gene sequence and its expression as a lmwRNA transcript across evolution. Southern blot analysis has shown that homologous genes of single or low copy number are found in all eukaryotes examined as well as in E. coli. Northern blot analysis has demonstrated 4.5S hybRNA transcription in all mouse tissues as well as expression in yeast and Xenopus laevis as lmwRNAs of approximately 130 and 100 nucleotides, respectively, as compared with mouse/rat/hamster species of approximately 87 nucleotides. Yeast and X. laevis 4.5S hybRNA homologs, isolated by hybrid-selection, were shown by Northern blot analysis to intermolecularly hybridize with homologous as well as heterologous 18S rRNA sequences. The conservation of 4.5S hybRNA homologous genes and their expression as lmwRNA transcripts with common intermolecular RNA:RNA hybridization capabilities in fungi, amphibians, and mammals argues for a common, conserved and required biological function for this lmwRNA in all eukaryotes and potential utilization of its intermolecular RNA:RNA hybridization capabilities to carry out this function.
???displayArticle.pubmedLink???
3399384 ???displayArticle.pmcLink???PMC336846 ???displayArticle.link???Nucleic Acids Res ???displayArticle.grants???[+]
Azad,
The 3'-terminal primary structure of five eukaryotic 18S rRNAs determined by the direct chemical method of sequencing. The highly conserved sequences include an invariant region complementary to eukaryotic 5S rRNA.
1980, Pubmed
Azad,
The 3'-terminal primary structure of five eukaryotic 18S rRNAs determined by the direct chemical method of sequencing. The highly conserved sequences include an invariant region complementary to eukaryotic 5S rRNA.
1980,
Pubmed Branlant,
Primary and secondary structures of Escherichia coli MRE 600 23S ribosomal RNA. Comparison with models of secondary structure for maize chloroplast 23S rRNA and for large portions of mouse and human 16S mitochondrial rRNAs.
1981,
Pubmed Bruce,
Reactions at the termini of tRNA with T4 RNA ligase.
1978,
Pubmed DUELL,
ISOLATION AND PROPERTIES OF INTACT MITOCHONDRIA FROM SPHEROPLASTS OF YEAST.
1964,
Pubmed Guerrier-Takada,
The RNA moiety of ribonuclease P is the catalytic subunit of the enzyme.
1983,
Pubmed Huysmans,
Compilation of small ribosomal subunit RNA sequences.
1986,
Pubmed Krämer,
The 5' terminus of the RNA moiety of U1 small nuclear ribonucleoprotein particles is required for the splicing of messenger RNA precursors.
1984,
Pubmed Lerner,
Are snRNPs involved in splicing?
1980,
Pubmed Maxwell,
Complementarity of sequences in low molecular weight RNAs to regions of messenger and ribosomal RNAs.
1986,
Pubmed Maxwell,
A low-molecular-weight RNA from mouse ascites cells that hybridizes to both 18S rRNA and mRNA sequences.
1986,
Pubmed McCarthy,
Characterization of translational-control ribonucleic acid isolated from embryonic chick muscle.
1983,
Pubmed Mizuno,
A unique mechanism regulating gene expression: translational inhibition by a complementary RNA transcript (micRNA).
1984,
Pubmed Nazar,
Role of the 5'-terminal sequence in the RNA binding site of yeast 5.8 S rRNA.
1980,
Pubmed Pace,
Structure of the 5.8S RNA component of the 5.8S-28S ribosomal RNA junction complex.
1977,
Pubmed Parker,
Recognition of the TACTAAC box during mRNA splicing in yeast involves base pairing to the U2-like snRNA.
1987,
Pubmed Peters,
Independent binding sites in mouse 5.8S ribosomal ribonucleic acid for 28S ribosomal ribonucleic acid.
1982,
Pubmed Schaufele,
Compensatory mutations suggest that base-pairing with a small nuclear RNA is required to form the 3' end of H3 messenger RNA.
,
Pubmed
,
Xenbase Seed,
Diazotizable arylamine cellulose papers for the coupling and hybridization of nucleic acids.
1982,
Pubmed Tomizawa,
Control of ColE1 plasmid replication: binding of RNA I to RNA II and inhibition of primer formation.
1986,
Pubmed Walker,
Enzymatic and chemical structure mapping of mouse 28S ribosomal ribonucleic acid contacts in 5.8S ribosomal ribonucleic acid.
1982,
Pubmed Walker,
Location of 5.8 S rRNA contact sites in 28 S rRNA and the effect of alpha-sarcin on the association of 5.8 S rRNA with 28 S rRNA.
1983,
Pubmed Wildeman,
Nucleotide sequence of wheat chloroplastid 4.5 S ribonucleic acid. Sequence homologies in 4.5 S RNA species.
1980,
Pubmed Zhuang,
A compensatory base change in U1 snRNA suppresses a 5' splice site mutation.
1986,
Pubmed
