Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
The Xenopus laevis mitochondrial RNA (mtRNA) polymerase was purified to near homogeneity with an overall yield approaching 50%. The major polypeptides in the final fraction were a doublet of proteins of approximately 140 kilodaltons that copurified with the mtRNA polymerase activity. It appeared likely that the smaller polypeptide is a breakdown product of the larger one. The highly purified polymerase was active in nonspecific transcription but required a dissociable factor for specific transcription of X. laevis mtDNA. The factor could be resolved from mtRNA polymerase by hydrophobic chromatography and had a sedimentation coefficient of 3.0 S. The transcription factor eluted from both the hydrophobic column and a Mono Q anion-exchange column as a single symmetrical peak. The mtRNA polymerase and this factor together are necessary and sufficient for active transcription from four promoters located in a noncoding region of the mtDNA genome between the gene for tRNA(Phe) and the displacement loop.
Anderson,
Sequence and organization of the human mitochondrial genome.
1981, Pubmed
Anderson,
Sequence and organization of the human mitochondrial genome.
1981,
Pubmed Biswas,
Characterization of a yeast mitochondrial promoter by deletion mutagenesis.
1985,
Pubmed Bogenhagen,
Identification of a promoter for transcription of the heavy strand of human mtDNA: in vitro transcription and deletion mutagenesis.
1984,
Pubmed Bogenhagen,
Template sequences required for transcription of Xenopus laevis mitochondrial DNA from two bidirectional promoters.
1988,
Pubmed
,
Xenbase Bogenhagen,
Accurate in vitro transcription of Xenopus laevis mitochondrial DNA from two bidirectional promoters.
1986,
Pubmed
,
Xenbase Cairns,
Mapping of the displacement loop within the nucleotide sequence of Xenopus laevis mitochondrial DNA.
1986,
Pubmed
,
Xenbase Chang,
Precise assignment of the heavy-strand promoter of mouse mitochondrial DNA: cognate start sites are not required for transcriptional initiation.
1986,
Pubmed Chang,
Precise identification of individual promoters for transcription of each strand of human mitochondrial DNA.
1984,
Pubmed Chang,
Precise assignment of the light-strand promoter of mouse mitochondrial DNA: a functional promoter consists of multiple upstream domains.
1986,
Pubmed Chomyn,
Six unidentified reading frames of human mitochondrial DNA encode components of the respiratory-chain NADH dehydrogenase.
,
Pubmed Fisher,
A transcription factor required for promoter recognition by human mitochondrial RNA polymerase. Accurate initiation at the heavy- and light-strand promoters dissected and reconstituted in vitro.
1985,
Pubmed Fisher,
Promoter selection in human mitochondria involves binding of a transcription factor to orientation-independent upstream regulatory elements.
1987,
Pubmed Kelly,
Yeast mitochondrial RNA polymerase. Purification and properties of the catalytic subunit.
1986,
Pubmed Kelly,
Isolation of the nuclear gene encoding a subunit of the yeast mitochondrial RNA polymerase.
1986,
Pubmed Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed Levens,
Novel method for identifying sequence-specific DNA-binding proteins.
1985,
Pubmed Levens,
Purification of mitochondrial RNA polymerase from Saccharomyces cerevisiae.
1981,
Pubmed Masters,
Yeast mitochondrial RNA polymerase is homologous to those encoded by bacteriophages T3 and T7.
1987,
Pubmed Reid,
Partial purification of mitochondrial RNA polymerase from rat liver.
1971,
Pubmed Schinkel,
Characterization of the promoter of the large ribosomal RNA gene in yeast mitochondria and separation of mitochondrial RNA polymerase into two different functional components.
1986,
Pubmed Shaltiel,
Hydrophobic chromatography.
1974,
Pubmed Shuey,
Characterization of an RNA polymerase activity from HeLa cell mitochondria, which initiates transcription at the heavy strand rRNA promoter and the light strand promoter in human mitochondrial DNA.
1985,
Pubmed Walberg,
In vitro transcription of human mitochondrial DNA. Identification of specific light strand transcripts from the displacement loop region.
1983,
Pubmed Winkley,
A multicomponent mitochondrial RNA polymerase from Saccharomyces cerevisiae.
1985,
Pubmed Wray,
Silver staining of proteins in polyacrylamide gels.
1981,
Pubmed Wu,
Purification and properties of mitochondrial deoxyribonucleic acid dependent ribonucleic acid polymerase from ovaries of Xenopus laevis.
1972,
Pubmed
,
Xenbase
