Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Early cross-striation formation in twitching Xenopus myocytes in culture.
Kidokoro Y, Saito M.
???displayArticle.abstract???
Spontaneous release of neurotransmitter has been demonstrated in various types of synapses. Its physiological significance, however, is still unknown. In nerve-muscle cultures of embryonic Xenopus laevis, we observed that acetylcholine, which is released spontaneously at the synaptic terminal, caused frequent twitches of muscle cells. These muscle cells developed cross-striations earlier than neighboring non-twitching cells. This effect of innervation was unaffected by tetrodotoxin but was blocked by alpha-bungarotoxin. Repeated iontophoretic application of acetylcholine or KCl to muscle cells caused twitches and also accelerated the formation of cross-striations. Thus twitching apparently promotes lateral alignment of myofibrils. It is also known that myosin synthesis is higher in twitching muscle cells. Therefore, successfully innervated twitching muscle cells may have an advantage for faster differentiation over neighboring non-twitching muscle cells. We suggest that spontaneously released transmitter may serve as a mediator for trophic interaction at forming synapses.
Anderson,
Effects of innervation on the distribution of acetylcholine receptors on cultured muscle cells.
1977, Pubmed,
Xenbase
Anderson,
Effects of innervation on the distribution of acetylcholine receptors on cultured muscle cells.
1977,
Pubmed
,
Xenbase Armstrong,
Muscle activity and the loss of electrical coupling between striated muscle cells in Xenopus embryos.
1983,
Pubmed
,
Xenbase Bandman,
Increased K+ inhibits spontaneous contractions reduces myosin accumulation in cultured chick myotubes.
1982,
Pubmed Bevan,
The distribution of alpha-bungarotoxin binding sites of mammalian skeletal muscle developing in vivo.
1977,
Pubmed Blackshaw,
Low resistance junctions between mesoderm cells during development of trunk muscles.
1976,
Pubmed
,
Xenbase Burden,
Acetylcholine receptors at the neuromuscular junction: developmental change in receptor turnover.
1977,
Pubmed Chow,
Developmental changes in the distribution of acetylcholine receptors in the myotomes of Xenopus laevis.
1983,
Pubmed
,
Xenbase Cohen,
In vivo development of cholinesterase at a neuromuscular junction in the absence of motor activity in Xenopus laevis.
1984,
Pubmed
,
Xenbase Danto,
Immunocytochemical analysis of intermediate filaments in embryonic heart cells with monoclonal antibodies to desmin.
1984,
Pubmed Dennis,
Development of the neuromuscular junction: inductive interactions between cells.
1981,
Pubmed
,
Xenbase DIAMOND,
A study of foetal and new-born rat muscle fibres.
1962,
Pubmed FATT,
Spontaneous subthreshold activity at motor nerve endings.
1952,
Pubmed Haydon,
Serotonin selectively inhibits growth cone motility and synaptogenesis of specific identified neurons.
1984,
Pubmed Hume,
Acetylcholine release from growth cones detected with patches of acetylcholine receptor-rich membranes.
,
Pubmed Kidokoro,
Initial synaptic transmission at the growth cone in Xenopus nerve-muscle cultures.
1982,
Pubmed
,
Xenbase Kidokoro,
Changes in synaptic potential properties during acetylcholine receptor accumulation and neurospecific interactions in Xenopus nerve-muscle cell culture.
1980,
Pubmed
,
Xenbase Kidokoro,
Two types of miniature endplate potentials in Xenopus nerve-muscle cultures.
1984,
Pubmed
,
Xenbase Klein,
Development of heart cells in culture: studies using an affinity purified antibody to a myosin light chain.
1985,
Pubmed Klier,
The ultrastructural differentiation of the clonal myogenic cell line L6 in normal and high K+ medium.
1977,
Pubmed Kullberg,
Development of the myotomal neuromuscular junction in Xenopus laevis: an electrophysiological and fine-structural study.
1977,
Pubmed
,
Xenbase Kuno,
Quantum aspects of central and ganglionic synaptic transmission in vertebrates.
1971,
Pubmed Lazarides,
Intermediate filaments as mechanical integrators of cellular space.
1980,
Pubmed McDermott,
Contraction regulates myosin synthesis and myosin content of cultured heart cells.
1985,
Pubmed Muntz,
Myogenesis in the trunk and leg during development of the tadpole of Xenopus laevis (Daudin 1802).
1975,
Pubmed
,
Xenbase Myers,
Development and axonal outgrowth of identified motoneurons in the zebrafish.
1986,
Pubmed Nakajima,
The development of functional neuromuscular junctions in vitro: an ultrastructural and physiological study.
1980,
Pubmed Peng,
The development of myofibrils in cultured muscle cells: a whole-mount and thin-section electron microscopic study.
1981,
Pubmed
,
Xenbase Tokuyasu,
Immunoelectron microscopic studies of desmin (skeletin) localization and intermediate filament organization in chicken skeletal muscle.
1983,
Pubmed Young,
Spontaneous release of transmitter from growth cones of embryonic neurones.
,
Pubmed
,
Xenbase
