Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Coupling of inositol phospholipid hydrolysis to peptide hormone receptors expressed from adrenal and pituitary mRNA in Xenopus laevis oocytes.
McIntosh RP, Catt KJ.
???displayArticle.abstract???
The expression of several neurotransmitter and drug receptors from injected exogenous mRNA in Xenopus laevis oocytes has been demonstrated by electrophysiological measurements of ion channel activation. The expression of specific receptors for peptide hormones in such a translation system would facilitate studies on the structure and regulation of cell-surface receptors as well as their coupling to membrane transduction mechanisms. The expression of receptors for calcium-mobilizing hormones in Xenopus oocytes was sought by analysis of phospholipid turnover in hormone-stimulated oocytes. For this purpose, Xenopus oocytes were injected with mRNA extracted from bovine adrenal and pituitary glands and incubated with myo-[3H]inositol to label plasma-membrane phosphatidylinositol phosphates. The expression of functionally active receptors for angiotensin II (AII) and thyrotropin-releasing hormone (TRH) was demonstrated by the stimulation of [3H]inositol phosphate production by AII and TRH in the mRNA-injected, [3H]inositol-prelabeled oocytes. The ability of AII and TRH to act by way of newly synthesized receptors from mammalian endocrine tissues to stimulate phosphatidylinositol polyphosphate hydrolysis in Xenopus oocytes suggests a generalized and conserved mechanism of receptor coupling to the transduction mechanism responsible for activation of phospholipase C in the plasma membrane.
Balla,
Angiotensin-stimulated production of inositol trisphosphate isomers and rapid metabolism through inositol 4-monophosphate in adrenal glomerulosa cells.
1986, Pubmed
Balla,
Angiotensin-stimulated production of inositol trisphosphate isomers and rapid metabolism through inositol 4-monophosphate in adrenal glomerulosa cells.
1986,
Pubmed Berridge,
Inositol trisphosphate, a novel second messenger in cellular signal transduction.
,
Pubmed Berridge,
Changes in the levels of inositol phosphates after agonist-dependent hydrolysis of membrane phosphoinositides.
1983,
Pubmed Brass,
Regulation of the phosphoinositide hydrolysis pathway in thrombin-stimulated platelets by a pertussis toxin-sensitive guanine nucleotide-binding protein. Evaluation of its contribution to platelet activation and comparisons with the adenylate cyclase inhibitory protein, Gi.
1986,
Pubmed Dixon,
Cloning of the gene and cDNA for mammalian beta-adrenergic receptor and homology with rhodopsin.
,
Pubmed Downes,
Inositol 1,3,4,5-tetrakisphosphate and not phosphatidylinositol 3,4-bisphosphate is the probable precursor of inositol 1,3,4-trisphosphate in agonist-stimulated parotid gland.
1986,
Pubmed Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase Guillemette,
Intracellular receptors for inositol 1,4,5-trisphosphate in angiotensin II target tissues.
1987,
Pubmed Gundersen,
Serotonin receptors induced by exogenous messenger RNA in Xenopus oocytes.
1983,
Pubmed
,
Xenbase Gurdon,
Use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells.
1971,
Pubmed
,
Xenbase Mendelsohn,
Autoradiographic localization of angiotensin II receptors in rat brain.
1984,
Pubmed Miledi,
Recording of single gamma-aminobutyrate- and acetylcholine-activated receptor channels translated by exogenous mRNA in Xenopus oocytes.
1983,
Pubmed
,
Xenbase Mishina,
Expression of functional acetylcholine receptor from cloned cDNAs.
,
Pubmed
,
Xenbase Morgan,
Novel aspects of gonadotropin-releasing hormone action on inositol polyphosphate metabolism in cultured pituitary gonadotrophs.
1987,
Pubmed Nakamura,
Simultaneous inhibitions of inositol phospholipid breakdown, arachidonic acid release, and histamine secretion in mast cells by islet-activating protein, pertussis toxin. A possible involvement of the toxin-specific substrate in the Ca2+-mobilizing receptor-mediated biosignaling system.
1985,
Pubmed NULL,
Proceedings of the symposium: GABA mechanisms and their clinical significance. Cambridge, U.K. 26-28 July, 1984.
1984,
Pubmed Oron,
Inositol 1,4,5-trisphosphate mimics muscarinic response in Xenopus oocytes.
,
Pubmed
,
Xenbase Preiss,
Quantitative measurement of sn-1,2-diacylglycerols present in platelets, hepatocytes, and ras- and sis-transformed normal rat kidney cells.
1986,
Pubmed Smart,
mRNA-directed synthesis and insertion of functional amino acid receptors in Xenopus laevis oocytes.
1987,
Pubmed
,
Xenbase Sumikawa,
Active multi-subunit ACh receptor assembled by translation of heterologous mRNA in Xenopus oocytes.
1981,
Pubmed
,
Xenbase Sumikawa,
Separate fractions of mRNA from Torpedo electric organ induce chloride channels and acetylcholine receptors in Xenopus oocytes.
1984,
Pubmed
,
Xenbase Ullrich,
Human insulin receptor and its relationship to the tyrosine kinase family of oncogenes.
,
Pubmed Ullrich,
Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells.
,
Pubmed Wakelam,
Normal p21N-ras couples bombesin and other growth factor receptors to inositol phosphate production.
,
Pubmed Wallace,
Protein incorporation by isolated amphibian oocytes. 3. Optimum incubation conditions.
1973,
Pubmed
,
Xenbase Worley,
Inositol trisphosphate receptor localization in brain: variable stoichiometry with protein kinase C.
,
Pubmed Zasloff,
tRNA transport from the nucleus in a eukaryotic cell: carrier-mediated translocation process.
1983,
Pubmed
,
Xenbase
