Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Transcriptionally inactive oocyte-type 5S RNA genes of Xenopus laevis are complexed with TFIIIA in vitro.
Peck LJ, Millstein L, Eversole-Cire P, Gottesfeld JM, Varshavsky A.
???displayArticle.abstract???
An extract from whole oocytes of Xenopus laevis was shown to transcribe somatic-type 5S RNA genes approximately 100-fold more efficiently than oocyte-type 5S RNA genes. This preference was at least 10-fold greater than the preference seen upon microinjection of 5S RNA genes into oocyte nuclei or upon in vitro transcription in an oocyte nuclear extract. The approximately 100-fold transcriptional bias in favor of the somatic-type 5S RNA genes observed in vitro in the whole oocyte extract was similar to the transcriptional bias observed in developing Xenopus embryos. We also showed that in the whole oocyte extract, a promoter-binding protein required for 5S RNA gene transcription, TFIIIA, was bound both to the actively transcribed somatic-type 5S RNA gene and to the largely inactive oocyte-type 5S RNA genes. These findings suggest that the mechanism for the differential expression of 5S RNA genes during Xenopus development does not involve differential binding of TFIIIA to 5S RNA genes.
Bieker,
Physical properties and DNA-binding stoichiometry of a 5 S gene-specific transcription factor.
1984, Pubmed,
Xenbase
Bieker,
Physical properties and DNA-binding stoichiometry of a 5 S gene-specific transcription factor.
1984,
Pubmed
,
Xenbase Birkenmeier,
A nuclear extract of Xenopus laevis oocytes that accurately transcribes 5S RNA genes.
1978,
Pubmed
,
Xenbase Bogenhagen,
Stable transcription complexes of Xenopus 5S RNA genes: a means to maintain the differentiated state.
1982,
Pubmed
,
Xenbase Bogenhagen,
Nucleotide sequences in Xenopus 5S DNA required for transcription termination.
1981,
Pubmed
,
Xenbase Brown,
The role of stable complexes that repress and activate eucaryotic genes.
1984,
Pubmed
,
Xenbase Brown,
How a simple animal gene works.
,
Pubmed
,
Xenbase Brown,
A positive transcription factor controls the differential expression of two 5S RNA genes.
1985,
Pubmed
,
Xenbase Engelke,
Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes.
1980,
Pubmed
,
Xenbase Gargiulo,
Assembly of transcriptionally active chromatin in Xenopus oocytes requires specific DNA binding factors.
1984,
Pubmed
,
Xenbase Gilbert,
Temporal order of replication of Xenopus laevis 5S ribosomal RNA genes in somatic cells.
1986,
Pubmed
,
Xenbase Ginsberg,
Xenopus 5S gene transcription factor, TFIIIA: characterization of a cDNA clone and measurement of RNA levels throughout development.
1984,
Pubmed
,
Xenbase Glikin,
Chromatin assembly in Xenopus oocytes: in vitro studies.
1984,
Pubmed
,
Xenbase Gottesfeld,
Assembly of transcriptionally active 5S RNA gene chromatin in vitro.
1982,
Pubmed
,
Xenbase Guinta,
Differential order of replication of Xenopus laevis 5S RNA genes.
1986,
Pubmed
,
Xenbase Kmiec,
The role of DNA-mediated transfer of TFIIIA in the concerted gyration and differential activation of the Xenopus 5S RNA genes.
1986,
Pubmed
,
Xenbase Korn,
Transcription of Xenopus 5S ribosomal RNA genes.
1982,
Pubmed
,
Xenbase Korn,
The reactivation of developmentally inert 5S genes in somatic nuclei injected into Xenopus oocytes.
1981,
Pubmed
,
Xenbase Lassar,
Transcription of class III genes: formation of preinitiation complexes.
1983,
Pubmed
,
Xenbase Maxam,
Sequencing end-labeled DNA with base-specific chemical cleavages.
1980,
Pubmed Miller,
Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes.
1985,
Pubmed
,
Xenbase Miller,
A transcriptionally active pseudogene in xenopus laevis oocyte 5S DNA.
1981,
Pubmed
,
Xenbase Miller,
The nucleotide sequence of oocyte 5S DNA in Xenopus laevis. II. The GC-rich region.
1978,
Pubmed
,
Xenbase Pelham,
A specific transcription factor that can bind either the 5S RNA gene or 5S RNA.
1980,
Pubmed
,
Xenbase Sakonju,
Contact points between a positive transcription factor and the Xenopus 5S RNA gene.
1982,
Pubmed
,
Xenbase Schlissel,
The transcriptional regulation of Xenopus 5s RNA genes in chromatin: the roles of active stable transcription complexes and histone H1.
1984,
Pubmed
,
Xenbase Segall,
Multiple factors are required for the accurate transcription of purified genes by RNA polymerase III.
1980,
Pubmed Setzer,
Formation and stability of the 5 S RNA transcription complex.
1985,
Pubmed
,
Xenbase Shastry,
Multiple factors involved in the transcription of class III genes in Xenopus laevis.
1982,
Pubmed
,
Xenbase Shastry,
Altered levels of a 5 S gene-specific transcription factor (TFIIIA) during oogenesis and embryonic development of Xenopus laevis.
1984,
Pubmed
,
Xenbase Teo,
The intracellular signal for induction of resistance to alkylating agents in E. coli.
1986,
Pubmed Wakefield,
Cytoplasmic regulation of 5S RNA genes in nuclear-transplant embryos.
1983,
Pubmed
,
Xenbase Wolffe,
A bacteriophage RNA polymerase transcribes through a Xenopus 5S RNA gene transcription complex without disrupting it.
1986,
Pubmed
,
Xenbase Wormington,
Onset of 5 S RNA gene regulation during Xenopus embryogenesis.
1983,
Pubmed
,
Xenbase Wormington,
A quantitative assay for Xenopus 5S RNA gene transcription in vitro.
1981,
Pubmed
,
Xenbase
