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XB-ART-28000
Dev Biol 1987 Sep 01;3883:263-7.
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Expression of the functional D-glucose transport system in Xenopus oocytes injected with mRNA of rat small intestine.

Aoshima H, Ishii H, Anan M.


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mRNA prepared from rat small intestine was injected into Xenopus oocytes. The injected oocytes showed a clear electrical response to D-glucose (Glu) in the form of membrane depolarization and conductance increase, while none was shown to D-fructose. The membrane electrical response of the injected oocytes evoked by Glu followed the Michaelis-Menten type kinetics and was dependent on the membrane potential of the oocyte. Replacing the Na+ of the bathing buffer with choline+ resulted in no response to Glu. Thus, a Glu transport system coupled to a Na+ gradient was expressed in Xenopus oocytes by injecting mRNA from rat small intestine.

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