Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
We have undertaken an immunological and biochemical analysis of the most abundant soluble protein of previtellogenic Xenopus oocytes, 42S p48. We show that this protein shares immunological cross-reactivity with elongation factor 1 alpha (EF-1 alpha). Direct assays of both 42S fractions and purified 42S p48 show that this cross-reactivity is of functional significance since 42S p48, like EF-1 alpha, can transfer charged amino acids to ribosomes. We further demonstrate that 42S p48 is degraded soon after the onset of vitellogenesis, while the EF-1 alpha concentration remains essentially unchanged during this transition. These properties of 42S p48 are discussed with regard to its role in oogenesis.
Brown,
Specific gene amplification in oocytes. Oocyte nuclei contain extrachromosomal replicas of the genes for ribosomal RNA.
1968, Pubmed
Brown,
Specific gene amplification in oocytes. Oocyte nuclei contain extrachromosomal replicas of the genes for ribosomal RNA.
1968,
Pubmed Cavallius,
Changes in activity and amount of active elongation factor 1 alpha in aging and immortal human fibroblast cultures.
1986,
Pubmed Coppard,
Identification of elongation factor 1 alpha from mouse liver.
1982,
Pubmed Denis,
Thesaurisomes, a novel kind of nucleoprotein particle.
1983,
Pubmed
,
Xenbase Dixon,
Regulation of protein synthesis and accumulation during oogenesis in Xenopus laevis.
1982,
Pubmed
,
Xenbase Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase Engelke,
Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes.
1980,
Pubmed
,
Xenbase Evans,
Localization of amplified ribosomal DNA in the oocyte of Xenopus laevis.
1968,
Pubmed
,
Xenbase Fonzi,
Regulation of protein synthesis factor EF-1 alpha in Mucor racemosus.
1985,
Pubmed Ford,
Non-coordinated accumulation and synthesis of 5S ribonucleic acid by ovaries of Xenopus laevis.
1971,
Pubmed
,
Xenbase Gall,
Differential synthesis of the genes for ribosomal RNA during amphibian oögenesis.
1968,
Pubmed Honda,
Association of a 5S gene transcription factor with 5S RNA and altered levels of the factor during cell differentiation.
1980,
Pubmed
,
Xenbase LOWRY,
Protein measurement with the Folin phenol reagent.
1951,
Pubmed Mairy,
[Biochemical studies on oogenesis. I. RNA synthesis and accumulation during oogenesis of the South African toad Xenopus laevis].
1971,
Pubmed
,
Xenbase Mattaj,
Nuclear exclusion of transcription factor IIIA and the 42s particle transfer RNA-binding protein in Xenopus oocytes: a possible mechanism for gene control?
1983,
Pubmed
,
Xenbase Merrick,
Assays for eukaryotic protein synthesis.
1979,
Pubmed Pelham,
A specific transcription factor that can bind either the 5S RNA gene or 5S RNA.
1980,
Pubmed
,
Xenbase Picard,
Isolation of a 7S particle from Xenopus laevis oocytes: a 5S RNA-protein complex.
1979,
Pubmed
,
Xenbase Picard,
Biochemical Research on oogenesis. Composition of the 42-S storage particles of Xenopus laevix oocytes.
1980,
Pubmed
,
Xenbase Pingoud,
Aminoacyl transfer ribonucleic acid binding site of the bacterial elongation factor Tu.
1980,
Pubmed Sakonju,
The binding of a transcription factor to deletion mutants of a 5S ribosomal RNA gene.
1981,
Pubmed
,
Xenbase Slobin,
Purification of elongation factor 1 from embryos of Artemia salina.
1979,
Pubmed Towbin,
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
1979,
Pubmed van Hemert,
The primary structure of elongation factor EF-1 alpha from the brine shrimp Artemia.
1984,
Pubmed
