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XB-ART-28066
EMBO J 1987 Jul 01;67:2003-10. doi: 10.1002/j.1460-2075.1987.tb02464.x.
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Periodic DNA synthesis in cell-free extracts of Xenopus eggs.

Hutchison CJ, Cox R, Drepaul RS, Gomperts M, Ford CC.


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Cell-free extracts prepared from unfertilized eggs of Xenopus laevis support DNA synthesis on sperm pronuclei. Continuous labelling studies using [3H]dCTP and pulse labelling studies using [32P]dCTP demonstrate that synthesis occurs in short bursts of 40 min, which are punctuated by periods of 20-40 min during which no synthesis occurs. Density substitution experiments using bromodeoxyuridine demonstrate that this synthesis involves the initiation of replication and reveals that re-initiation events can occur following multiple bursts of replication. The periodic properties of these extracts are sensitive to protein synthesis inhibitors.

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Species referenced: Xenopus laevis

References [+] :
Blow, Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs. 1986, Pubmed, Xenbase