Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
By immunocytochemistry, quantitative immunoblotting, and two-dimensional gel electrophoresis, we have analyzed the distribution of nuclear lamin proteins during chicken embryonic development. Whereas no qualitative differences in the patterns of expression of lamins A, B1, and B2 were observed during gametogenesis in either the female or the male germ line, profound changes in the composition of the nuclear lamina occurred during the development of somatic tissues. Most unexpectedly, early chicken embryos were found to contain little if any lamin A, although they contained substantial amounts of lamins B1 and B2. During embryonic development, lamin A became increasingly prominent, whereas the amounts of lamin B1 decreased in many tissues. Interestingly, the extent and the developmental timing of these changes displayed pronounced tissue-specific variations. Lamin B2 was expressed in fairly constant amounts in all cell types investigated (except for pachytene-stage germ cells). These results have implications for the purported functional specializations of individual lamin proteins. In addition, they suggest that alterations in the composition of the nuclear lamina may be important for the establishment of cell- or tissue-specific differences in nuclear architecture.
Aebi,
The nuclear lamina is a meshwork of intermediate-type filaments.
, Pubmed,
Xenbase
Aebi,
The nuclear lamina is a meshwork of intermediate-type filaments.
,
Pubmed
,
Xenbase Benavente,
Change of karyoskeleton during spermatogenesis of Xenopus: expression of lamin LIV, a nuclear lamina protein specific for the male germ line.
1985,
Pubmed
,
Xenbase Benavente,
Involvement of nuclear lamins in postmitotic reorganization of chromatin as demonstrated by microinjection of lamin antibodies.
1986,
Pubmed Benavente,
Cell type-specific expression of nuclear lamina proteins during development of Xenopus laevis.
1985,
Pubmed
,
Xenbase Blobel,
Gene gating: a hypothesis.
1985,
Pubmed Blobel,
Nuclei from rat liver: isolation method that combines purity with high yield.
1966,
Pubmed Burke,
A cell free system to study reassembly of the nuclear envelope at the end of mitosis.
1986,
Pubmed Dwyer,
A modified procedure for the isolation of a pore complex-lamina fraction from rat liver nuclei.
1976,
Pubmed Fisher,
cDNA sequencing of nuclear lamins A and C reveals primary and secondary structural homology to intermediate filament proteins.
1986,
Pubmed Gerace,
Immunocytochemical localization of the major polypeptides of the nuclear pore complex-lamina fraction. Interphase and mitotic distribution.
1978,
Pubmed Gerace,
The nuclear envelope lamina is reversibly depolymerized during mitosis.
1980,
Pubmed Gerace,
Organization and modulation of nuclear lamina structure.
1984,
Pubmed Gerace,
Nuclear lamina and the structural organization of the nuclear envelope.
1982,
Pubmed Giger,
Induction of delta-aminolevulinate synthase and cytochrome P-450 hemoproteins in hepatocyte culture. Effect of glucose and hormones.
1981,
Pubmed Ingram,
Embryonic red blood cell formation.
1972,
Pubmed Krohne,
The nuclear lamins. A multigene family of proteins in evolution and differentiation.
1986,
Pubmed
,
Xenbase Krohne,
A monoclonal antibody against nuclear lamina proteins reveals cell type-specificity in Xenopus laevis.
1984,
Pubmed
,
Xenbase Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed Lebkowski,
Non-histone proteins and long-range organization of HeLa interphase DNA.
1982,
Pubmed Lehner,
The nuclear lamin protein family in higher vertebrates. Identification of quantitatively minor lamin proteins by monoclonal antibodies.
1986,
Pubmed Lehner,
Biogenesis of the nuclear lamina: in vivo synthesis and processing of nuclear protein precursors.
1986,
Pubmed Lehner,
Nuclear substructure antigens. Monoclonal antibodies against components of nuclear matrix preparations.
1986,
Pubmed Maul,
Identification and redistribution of lamins during nuclear differentiation in mouse spermatogenesis.
1986,
Pubmed McKeon,
Homologies in both primary and secondary structure between nuclear envelope and intermediate filament proteins.
,
Pubmed Miake-Lye,
Induction of early mitotic events in a cell-free system.
1985,
Pubmed
,
Xenbase Nigg,
Cyclic-AMP-dependent protein kinase type II is associated with the Golgi complex and with centrosomes.
1985,
Pubmed O'Farrell,
High resolution two-dimensional electrophoresis of basic as well as acidic proteins.
1977,
Pubmed Ottaviano,
Phosphorylation of the nuclear lamins during interphase and mitosis.
1985,
Pubmed Schatten,
Nuclear lamins and peripheral nuclear antigens during fertilization and embryogenesis in mice and sea urchins.
1985,
Pubmed Smith,
Identification, developmental regulation, and response to heat shock of two antigenically related forms of a major nuclear envelope protein in Drosophila embryos: application of an improved method for affinity purification of antibodies using polypeptides immobilized on nitrocellulose blots.
1984,
Pubmed Sommer,
Monoclonal antibodies (O1 to O4) to oligodendrocyte cell surfaces: an immunocytological study in the central nervous system.
1981,
Pubmed Stick,
The disappearance of the nuclear lamina during spermatogenesis: an electron microscopic and immunofluorescence study.
1982,
Pubmed Stick,
Disappearance and reformation of the nuclear lamina structure during specific stages of meiosis in oocytes.
1983,
Pubmed Stick,
Changes in the nuclear lamina composition during early development of Xenopus laevis.
1985,
Pubmed
,
Xenbase Towbin,
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
1979,
Pubmed
