Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
Selective transport of proteins is a major mechanism by which biochemical differences are maintained between the cytoplasm and nucleus. To begin to investigate the molecular mechanism of nuclear transport, we used an in vitro transport system composed of a Xenopus egg extract, rat liver nuclei, and a fluorescently labeled nuclear protein, nucleoplasmin. With this system, we screened for inhibitors of transport. We found that the lectin, wheat germ agglutinin (WGA), completely inhibits the nuclear transport of fluorescently labeled nucleoplasmin. No other lectin tested affected nuclear transport. The inhibition by WGA was not seen when N-acetylglucosamine was present and was reversible by subsequent addition of sugar. When rat liver nuclei that had been incubated with ferritin-labeled WGA were examined by electron microscopy, multiple molecules of WGA were found bound to the cytoplasmic face of each nuclear pore. Gel electrophoresis and nitrocellulose transfer identified one major and several minor nuclear protein bands as binding 125I-labeled WGA. The most abundant protein of these, a 63-65-kD glycoprotein, is a candidate for the inhibitory site of action of WGA on nuclear protein transport. WGA is the first identified inhibitor of nuclear protein transport and interacts directly with the nuclear pore.
Aaronson,
On the attachment of the nuclear pore complex.
1974, Pubmed
Aaronson,
On the attachment of the nuclear pore complex.
1974,
Pubmed Agutter,
Importance of mammalian nuclear-envelope nucleoside triphosphatase in nucleo-cytoplasmic transport of ribonucleoproteins.
1979,
Pubmed Baglia,
Nuclear ribonucleoprotein release and nucleoside triphosphatase activity are inhibited by antibodies directed against one nuclear matrix glycoprotein.
1983,
Pubmed Bartles,
125I-wheat germ agglutinin blotting: increased sensitivity with polyvinylpyrrolidone quenching and periodate oxidation/reductive phenylamination.
1984,
Pubmed Berrios,
Characterization of an ATPase/dATPase activity associated with the Drosophila nuclear matrix-pore complex-lamina fraction. Identification of the putative enzyme polypeptide by direct ultraviolet photoaffinity labeling.
1983,
Pubmed Berrios,
A myosin heavy-chain-like polypeptide is associated with the nuclear envelope in higher eukaryotic cells.
1986,
Pubmed Blobel,
Nuclei from rat liver: isolation method that combines purity with high yield.
1966,
Pubmed Bonner,
Protein migration into nuclei. I. Frog oocyte nuclei in vivo accumulate microinjected histones, allow entry to small proteins, and exclude large proteins.
1975,
Pubmed
,
Xenbase Davis,
Identification and characterization of a nuclear pore complex protein.
1986,
Pubmed De Robertis,
Nucleocytoplasmic segregation of proteins and RNAs.
1983,
Pubmed Dingwall,
A polypeptide domain that specifies migration of nucleoplasmin into the nucleus.
1982,
Pubmed
,
Xenbase Dwyer,
A modified procedure for the isolation of a pore complex-lamina fraction from rat liver nuclei.
1976,
Pubmed Einck,
Functional histone antibody fragments traverse the nuclear envelope.
1984,
Pubmed Feldherr,
Mechanism for the selection of nuclear polypeptides in Xenopus oocytes. II. Two-dimensional gel analysis.
1980,
Pubmed
,
Xenbase Feldherr,
Movement of a karyophilic protein through the nuclear pores of oocytes.
1984,
Pubmed
,
Xenbase Feldherr,
The distribution of ConA-binding sites on oocyte nuclear envelopes.
1977,
Pubmed
,
Xenbase Forbes,
Spontaneous formation of nucleus-like structures around bacteriophage DNA microinjected into Xenopus eggs.
1983,
Pubmed
,
Xenbase Franke,
The nuclear envelope and the architecture of the nuclear periphery.
1981,
Pubmed Gerace,
Identification of a major polypeptide of the nuclear pore complex.
1982,
Pubmed Hall,
Targeting of E. coli beta-galactosidase to the nucleus in yeast.
1984,
Pubmed Holt,
The subcellular distribution of terminal N-acetylglucosamine moieties. Localization of a novel protein-saccharide linkage, O-linked GlcNAc.
1986,
Pubmed Hozier,
Binding of lectins to mitotic chromosomes and interphase nuclear substructures.
1980,
Pubmed Jiang,
Chemical factors that influence nucleocytoplasmic transport: a fluorescence photobleaching study.
1986,
Pubmed Kalderon,
A short amino acid sequence able to specify nuclear location.
1984,
Pubmed Kan,
Preferential association of glycoproteins to the euchromatin regions of cross-fractured nuclei is revealed by fracture-label.
1986,
Pubmed Kornfeld,
Comparative aspects of glycoprotein structure.
1976,
Pubmed Krohne,
Immunological identification and localization of the predominant nuclear protein of the amphibian oocyte nucleus.
1980,
Pubmed
,
Xenbase Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed Lohka,
Formation in vitro of sperm pronuclei and mitotic chromosomes induced by amphibian ooplasmic components.
1983,
Pubmed
,
Xenbase Maul,
The nuclear and the cytoplasmic pore complex: structure, dynamics, distribution, and evolution.
1977,
Pubmed McKeon,
Autoimmune response directed against conserved determinants of nuclear envelope proteins in a patient with linear scleroderma.
1983,
Pubmed Mills,
An acidic protein which assembles nucleosomes in vitro is the most abundant protein in Xenopus oocyte nuclei.
1980,
Pubmed
,
Xenbase Newmeyer,
Assembly in vitro of nuclei active in nuclear protein transport: ATP is required for nucleoplasmin accumulation.
1986,
Pubmed
,
Xenbase Newmeyer,
In vitro transport of a fluorescent nuclear protein and exclusion of non-nuclear proteins.
1986,
Pubmed
,
Xenbase Newport,
The role of mitotic factors in regulating the timing of the midblastula transition in Xenopus.
1985,
Pubmed
,
Xenbase Nicolson,
Outer membrane terminal saccharides of bovine liver nuclei and mitochondria.
1972,
Pubmed Paine,
Nuclear envelope permeability.
1975,
Pubmed Schindler,
Lateral diffusion in nuclear membranes.
1985,
Pubmed Seve,
In situ distribution in different cell types of nuclear glycoconjugates detected by two lectins.
1984,
Pubmed Spurr,
A low-viscosity epoxy resin embedding medium for electron microscopy.
1969,
Pubmed Stevens,
RNA transport from nucleus to cytoplasm in Chironomus salivary glands.
1966,
Pubmed Torres,
Topography and polypeptide distribution of terminal N-acetylglucosamine residues on the surfaces of intact lymphocytes. Evidence for O-linked GlcNAc.
1984,
Pubmed Unwin,
A large particle associated with the perimeter of the nuclear pore complex.
1982,
Pubmed
,
Xenbase Virtanen,
Distribution of lectin binding sites on rat liver cell nuclei: comparison of fluorescein- and ferritin-labelling methods.
1978,
Pubmed Virtanen,
Lectin receptor sites on rat liver cell nuclear membranes.
1976,
Pubmed
