Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Neurotensin and acetylcholine evoke common responses in frog oocytes injected with rat brain messenger ribonucleic acid.
Hirono C, Ito I, Sugiyama H.
???displayArticle.abstract???
1. The intracellular reaction mechanism underlying electrophysiological responses evoked by neurotensin (NT) was studied using Xenopus laevis oocytes injected with poly (A)+ messenger ribonucleic acid (mRNA) isolated from rat brains. 2. A few days after the injection of mRNA, oocytes were found to acquire sensitivity to NT and substance P. 3. Under voltage-clamp conditions (-60 mV), application of NT to mRNA-injected oocytes produced transient and oscillatory inward currents which began after a delay of several tens of seconds. These inward currents were accompanied by an increase in membrane conductance. 4. NT receptors on mRNA-injected oocytes showed essentially the same pharmacological properties as those of native NT receptors. 5. The NT response showed desensitization and was not readily recovered even after extensive washing of cells for more than 30 min. 6. NT response was suppressed when the muscarinic acetylcholine (ACh) response of the same cell, which was also induced by the same mRNA, was desensitized by a large dose of ACh. 7. NT response and ACh response showed many similarities: they were both inhibited by pertussis toxin and intracellular ethyleneglycol-bis-(beta-aminoethylether) N, N'-tetraacetic acid (EGTA), mimicked by intracellularly injected inositol 1, 4, 5-trisphosphate (InsP3), and suppressed when cell response to InsP3 was desensitized by a large dose of InsP3. Reversal-potential analyses indicated that both responses were mediated by an increase in membrane permeability to Cl-. 8. It is concluded that NT responses and muscarinic ACh responses of Xenopus oocytes induced by rat brain mRNA may most likely share a common reaction mechanism. The reaction sequence includes the activation of receptors, activation of inhibitory guanine nucleotide-binding regulatory protein, production of InsP3, intracellular Ca2+ mobilization, and increased membrane permeability to Cl-.
Amar,
Regulation of cyclic GMP levels by neurotensin in neuroblastoma clone N1E115.
1985, Pubmed
Amar,
Regulation of cyclic GMP levels by neurotensin in neuroblastoma clone N1E115.
1985,
Pubmed Araki,
Isolation and structure of a new active peptide xenopsin on rat stomach strip and some biogenic amines in the skin of Xenopus laevis.
1975,
Pubmed
,
Xenbase Baldino,
Structure-activity studies with carboxy- and amino-terminal fragments of neurotensin on hypothalamic neurons in vitro.
1985,
Pubmed Checler,
[TRP11]-neurotensin and xenopsin discriminate between rat and guinea-pig neurotensin receptors.
1982,
Pubmed
,
Xenbase Dascal,
Role of calcium mobilization in mediation of acetylcholine-evoked chloride currents in Xenopus laevis oocytes.
1985,
Pubmed
,
Xenbase Dascal,
Xenopus oocyte resting potential, muscarinic responses and the role of calcium and guanosine 3',5'-cyclic monophosphate.
1984,
Pubmed
,
Xenbase Gorden,
Epidermal growth factor: morphological demonstration of binding, internalization, and lysosomal association in human fibroblasts.
1978,
Pubmed Gundersen,
Messenger RNA from human brain induces drug- and voltage-operated channels in Xenopus oocytes.
,
Pubmed
,
Xenbase Haga,
Functional reconstitution of purified muscarinic receptors and inhibitory guanine nucleotide regulatory protein.
,
Pubmed Hirono,
Characterization of mRNA responsible for induction of functional sodium channels in Xenopus oocytes.
1985,
Pubmed
,
Xenbase Houamed,
Expression of functional GABA, glycine and glutamate receptors in Xenopus oocytes injected with rat brain mRNA.
,
Pubmed
,
Xenbase Kusano,
Cholinergic and catecholaminergic receptors in the Xenopus oocyte membrane.
1982,
Pubmed
,
Xenbase Oron,
Inositol 1,4,5-trisphosphate mimics muscarinic response in Xenopus oocytes.
,
Pubmed
,
Xenbase Parker,
Intracellular Ca2+-dependent and Ca2+-independent responses of rat brain serotonin receptors transplanted to Xenopus oocytes.
1985,
Pubmed
,
Xenbase Pastan,
Receptor-mediated endocytosis of hormones in cultured cells.
1981,
Pubmed Quirion,
Selective blockade of neurotensin-induced coronary vessel constriction in perfused rat hearts by a neurotensin analogue.
1980,
Pubmed Rioux,
Pharmacological characterization of neurotensin receptors in the rat isolated portal vein using analogues and fragments of neurotensin.
1980,
Pubmed Sibley,
Molecular mechanisms of receptor desensitization using the beta-adrenergic receptor-coupled adenylate cyclase system as a model.
,
Pubmed Sternweis,
Isolation of two proteins with high affinity for guanine nucleotides from membranes of bovine brain.
1984,
Pubmed Sugiyama,
Induction of muscarinic cholinergic responsiveness in Xenopus oocytes by mRNA isolated from rat brain.
1985,
Pubmed
,
Xenbase Sumikawa,
Partial purification and functional expression of brain mRNAs coding for neurotransmitter receptors and voltage-operated channels.
1984,
Pubmed
,
Xenbase Sumikawa,
Active multi-subunit ACh receptor assembled by translation of heterologous mRNA in Xenopus oocytes.
1981,
Pubmed
,
Xenbase
