Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
A Xenopus cDNA clone highly homologous to the proto-oncogene c-myc has been isolated and used to derive a homologous probe to study myc expression during embryonic development. Myc RNA is identified as a member of the class of maternal mRNAs expressed before fertilisation. It is highly accumulated from early oogenesis and an unfertilised egg contains 8 pg, about 10(5)-fold the myc content of proliferative somatic cells. After fertilisation a post-transcriptional regulation of the gene is induced and the accumulated myc RNA is degraded (t1/2 = 4 h 20 min) to reach a level at gastrula of 10 transcripts per cell; a value maintained during subsequent embryonic development. The Xenopus myc protein has also been identified by both myc-specific antibodies and hybrid selection experiments. Translation in vitro of Xenopus myc RNA shows that it encodes a 62-kd protein which is also recognised by myc antibodies in oocyte extracts. This protein is accumulated in late oogenesis. The results indicate an unusual uncoupling of myc expression and cell proliferation linked to a stabilisation of the RNA product.
Auffray,
Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA.
1980, Pubmed
Auffray,
Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA.
1980,
Pubmed Bernard,
Sequence of the murine and human cellular myc oncogenes and two modes of myc transcription resulting from chromosome translocation in B lymphoid tumours.
1983,
Pubmed Bisbee,
Albumin phylogeny for clawed frogs (Xenopus).
1977,
Pubmed
,
Xenbase Blanchard,
c-myc gene is transcribed at high rate in G0-arrested fibroblasts and is post-transcriptionally regulated in response to growth factors.
,
Pubmed BRACHET,
Effects of actinomycin D on morphogenesis.
1963,
Pubmed Brown,
Synthesis and accumulation of low molecular weight RNA during embryogenesis of Xenopus laevis.
1966,
Pubmed
,
Xenbase BROWN,
RNA SYNTHESIS DURING THE DEVELOPMENT OF XENOPUS LAEVIS, THE SOUTH AFRICAN CLAWED TOAD.
1964,
Pubmed
,
Xenbase Cabada,
Differential accumulation of two size classes of poly(A) associated with messenger RNA during oogenesis in Xenopus laevis.
1977,
Pubmed
,
Xenbase Dalla-Favera,
Cloning and characterization of different human sequences related to the onc gene (v-myc) of avian myelocytomatosis virus (MC29).
1982,
Pubmed Dani,
Extreme instability of myc mRNA in normal and transformed human cells.
1984,
Pubmed Dawid,
Deoxyribonucleic acid in amphibian eggs.
1965,
Pubmed Dolecki,
Poly(A)+ RNA metabolism during oogenesis in Xenopus laevis.
1979,
Pubmed
,
Xenbase Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase Evan,
Studies on the interaction of the human c-myc protein with cell nuclei: p62c-myc as a member of a discrete subset of nuclear proteins.
1985,
Pubmed Ford,
Non-coordinated accumulation and synthesis of 5S ribonucleic acid by ovaries of Xenopus laevis.
1971,
Pubmed
,
Xenbase Golden,
Accumulation of individual pA+ RNAs during oogenesis of Xenopus laevis.
1980,
Pubmed
,
Xenbase Greenberg,
Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogene.
,
Pubmed Gurdon,
Transcription of muscle-specific actin genes in early Xenopus development: nuclear transplantation and cell dissociation.
1984,
Pubmed
,
Xenbase Gurdon,
The use of Xenopus oocytes for the expression of cloned genes.
1983,
Pubmed
,
Xenbase Heldin,
Growth factors: mechanism of action and relation to oncogenes.
1984,
Pubmed Henikoff,
Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing.
1984,
Pubmed Kelly,
Cell-specific regulation of the c-myc gene by lymphocyte mitogens and platelet-derived growth factor.
1983,
Pubmed Kingston,
Transcription control by oncogenes.
1985,
Pubmed Kohl,
Human N-myc is closely related in organization and nucleotide sequence to c-myc.
,
Pubmed Méchali,
Lack of specific sequence requirement for DNA replication in Xenopus eggs compared with high sequence specificity in yeast.
1984,
Pubmed
,
Xenbase Messing,
New M13 vectors for cloning.
1983,
Pubmed Newport,
A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage.
1982,
Pubmed
,
Xenbase Perlman,
Analysis of Xenopus laevis ovary and somatic cell polyadenylated RNA by molecular hybridization.
1978,
Pubmed
,
Xenbase Persson,
Antibodies to human c-myc oncogene product: evidence of an evolutionarily conserved protein induced during cell proliferation.
1984,
Pubmed Pfeifer-Ohlsson,
Spatial and temporal pattern of cellular myc oncogene expression in developing human placenta: implications for embryonic cell proliferation.
1984,
Pubmed Rebagliati,
Identification and cloning of localized maternal RNAs from Xenopus eggs.
1985,
Pubmed
,
Xenbase Reed,
Rapid transfer of DNA from agarose gels to nylon membranes.
1985,
Pubmed Ricciardi,
Purification and mapping of specific mRNAs by hybridization-selection and cell-free translation.
1979,
Pubmed Robert-Lezenes,
Expression of c-ras and c-myc oncogenes in murine erythroleukemias induced by Friend viruses.
1984,
Pubmed Rosbash,
Polyadenylic acid-containing RNA in Xenopus laevis oocytes.
1974,
Pubmed
,
Xenbase Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed van Dongen,
Quantitation of the accumulation of histone messenger RNA during oogenesis in Xenopus laevis.
1981,
Pubmed
,
Xenbase Woodland,
Utilization of stored mRNA in Xenopus embryos and its replacement by newly synthesized transcripts: histone H1 synthesis using interspecies hybrids.
1979,
Pubmed
,
Xenbase
