Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
1986 Dec 20;513:3547-52. doi: 10.1002/j.1460-2075.1986.tb04681.x.
Show Gene links
Show Anatomy links
Molecular characterization of a karyophilic, histone-binding protein: cDNA cloning, amino acid sequence and expression of nuclear protein N1/N2 of Xenopus laevis.
Kleinschmidt JA, Dingwall C, Maier G, Franke WW.
???displayArticle.abstract???
In the amphibian oocyte, most of the non-chromatin-bound histones are not free but form complexes with specific karyophilic proteins, the most prominent being nucleoplasmin and 'protein N1/N2'. Using antibodies against polypeptide N1 and N2 (Mr approximately 105,000 and approximately 110,000) we have isolated, from a Xenopus laevis ovary lambda gt11 expression library, several full length cDNA clones encoding one of the two closely related polypeptides N1 and N2 (these could not be distinguished by hybridization techniques). The amino acid sequence deduced from one of these clones (N1/N2, lambda 106.2) defines a polypeptide of mol. wt 64,774. The remarkably high difference between the value of Mr approximately 110,000 estimated from SDS-PAGE mobility and the true mol. wt has been found for (i) the cell protein, (ii) the polypeptide synthesized in vitro by transcription and translation and (iii) the fusion protein with beta-galactosidase expressed in Escherichia coli, indicating that the protein runs anomalously on SDS-PAGE. The amino and carboxy termini of the purified protein N1/N2 have been confirmed by direct amino acid sequencing of CNBr fragments. The amino acid sequence displays two glutamic acid-rich domains, which are probably involved in the interaction with the histones, and a putative nuclear targeting signal with high homology to that of the SV40 large T-antigen which is located near the carboxy terminus.(ABSTRACT TRUNCATED AT 250 WORDS)
Ballantine,
Changes in protein synthesis during the development of Xenopus laevis.
1979, Pubmed,
Xenbase
Ballantine,
Changes in protein synthesis during the development of Xenopus laevis.
1979,
Pubmed
,
Xenbase Bonne-Andrea,
Rat liver HMG1: a physiological nucleosome assembly factor.
1984,
Pubmed Bonner,
Protein migration into nuclei. II. Frog oocyte nuclei accumulate a class of microinjected oocyte nuclear proteins and exclude a class of microinjected oocyte cytoplasmic proteins.
1975,
Pubmed
,
Xenbase Colberg-Poley,
Structural analysis of murine genes containing homoeo box sequences and their expression in embryonal carcinoma cells.
,
Pubmed Dabauvalle,
Karyophilic proteins: polypeptides synthesized in vitro accumulate in the nucleus on microinjection into the cytoplasm of amphibian oocytes.
1982,
Pubmed
,
Xenbase De Robertis,
Intracellular migration of nuclear proteins in Xenopus oocytes.
1978,
Pubmed
,
Xenbase Dingwall,
A polypeptide domain that specifies migration of nucleoplasmin into the nucleus.
1982,
Pubmed
,
Xenbase Dreyer,
Two-dimensional gel analysis of the fate of oocyte nuclear proteins in the development of Xenopus laevis.
1983,
Pubmed
,
Xenbase Earnshaw,
Assembly of nucleosomes: the reaction involving X. laevis nucleoplasmin.
1980,
Pubmed
,
Xenbase Feldherr,
Evidence for mediated protein uptake by amphibian oocyte nuclei.
1983,
Pubmed Franz,
Cloning of cDNA and amino acid sequence of a cytokeratin expressed in oocytes of Xenopus laevis.
1986,
Pubmed
,
Xenbase Geisler,
Neurofilament architecture combines structural principles of intermediate filaments with carboxy-terminal extensions increasing in size between triplet proteins.
1983,
Pubmed Goldfarb,
Synthetic peptides as nuclear localization signals.
,
Pubmed
,
Xenbase Gubler,
A simple and very efficient method for generating cDNA libraries.
1983,
Pubmed Hall,
Targeting of E. coli beta-galactosidase to the nucleus in yeast.
1984,
Pubmed Henikoff,
Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing.
1984,
Pubmed Jorcano,
Cell type-specific expression of bovine keratin genes as demonstrated by the use of complementary DNA clones.
1984,
Pubmed Kalderon,
A short amino acid sequence able to specify nuclear location.
1984,
Pubmed Kaufmann,
SDS-PAGE strongly overestimates the molecular masses of the neurofilament proteins.
1984,
Pubmed Kleinschmidt,
Co-existence of two different types of soluble histone complexes in nuclei of Xenopus laevis oocytes.
1985,
Pubmed
,
Xenbase Kleinschmidt,
Soluble acidic complexes containing histones H3 and H4 in nuclei of Xenopus laevis oocytes.
1982,
Pubmed
,
Xenbase Kozak,
Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes.
1986,
Pubmed Krohne,
Immunological identification of the karyophilic, histone-binding proteins N1 and N2 in somatic cells and oocytes of diverse amphibia.
1985,
Pubmed
,
Xenbase Krohne,
A major soluble acidic protein located in nuclei of diverse vertebrate species.
1980,
Pubmed
,
Xenbase Krohne,
Immunological identification and localization of the predominant nuclear protein of the amphibian oocyte nucleus.
1980,
Pubmed
,
Xenbase Lanford,
Induction of nuclear transport with a synthetic peptide homologous to the SV40 T antigen transport signal.
1986,
Pubmed Laskey,
Nucleosomes are assembled by an acidic protein which binds histones and transfers them to DNA.
1978,
Pubmed
,
Xenbase Lewis,
Genetics, evolution, and expression of the 68,000-mol-wt neurofilament protein: isolation of a cloned cDNA probe.
1985,
Pubmed Magin,
Translational products of mRNAs coding for non-epidermal cytokeratins.
1983,
Pubmed
,
Xenbase McMaster,
Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.
1977,
Pubmed Mills,
An acidic protein which assembles nucleosomes in vitro is the most abundant protein in Xenopus oocyte nuclei.
1980,
Pubmed
,
Xenbase O'Farrell,
High resolution two-dimensional electrophoresis of proteins.
1975,
Pubmed Paine,
Diffusive and nondiffusive proteins in vivo.
1984,
Pubmed
,
Xenbase Ponstingl,
Amino acid sequence of alpha- and beta-tubulins from pig brain: heterogeneity and regional similarity to muscle proteins.
1982,
Pubmed Ricciardi,
Purification and mapping of specific mRNAs by hybridization-selection and cell-free translation.
1979,
Pubmed Richardson,
Nuclear location signals in polyoma virus large-T.
1986,
Pubmed Romano,
Cytokeratin expression in simple epithelia. I. Identification of mRNA coding for human cytokeratin no. 18 by a cDNA clone.
1986,
Pubmed Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed Stein,
Acidic polypeptides can assemble both histones and chromatin in vitro at physiological ionic strength.
1979,
Pubmed Thomas,
An octamer of histones in chromatin and free in solution.
1975,
Pubmed Vieira,
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
1982,
Pubmed Wedlich,
Occurrence of a species-specific nuclear antigen in the germ line of Xenopus and its expression from paternal genes in hybrid frogs.
1985,
Pubmed
,
Xenbase Wilkinson,
The expression of multiple forms of troponin T in chicken-fast-skeletal muscle may result from differential splicing of a single gene.
1984,
Pubmed Young,
Yeast RNA polymerase II genes: isolation with antibody probes.
1983,
Pubmed
