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The developmental expression of the gene for TFIIIA in Xenopus laevis.
Taylor W, Jackson IJ, Siegel N, Kumar A, Brown DD.
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The sequence of the trans-acting positive transcription factor TFIIIA has been deduced by sequencing cDNA and genomic DNA clones. Using DNA from a homozygous diploid animal we show that there is one gene for TFIIIA per haploid genome of X. laevis. Protein sequencing of proteolytic fragments of TFIIIA orients the protein in its interaction with the internal control region (ICR) of the 5S RNA gene. The protein lies along the DNA with its carboxyl terminus at the 5' end of the ICR and its amino terminus at the 3' end. The developmental pattern of TFIIIA and its mRNA during oogenesis and embryogenesis are consistent with the idea that the abundance of TFIIIA plays an essential role in the developmental change in 5S RNA gene expression. The change to almost exclusive somatic 5S RNA gene expression by gastrulation occurs using either the TFIIIA that was synthesized by oocyte and/or TFIIIA synthesized from maternal mRNA.
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3755818 ???displayArticle.pmcLink???PMC311630 ???displayArticle.link???Nucleic Acids Res ???displayArticle.grants???[+]
Aviv,
Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
1972, Pubmed
Aviv,
Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
1972,
Pubmed Bailey,
Methylmercury as a reversible denaturing agent for agarose gel electrophoresis.
1976,
Pubmed Brown,
A positive transcription factor controls the differential expression of two 5S RNA genes.
1985,
Pubmed
,
Xenbase Chirgwin,
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
1979,
Pubmed Engelke,
Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes.
1980,
Pubmed
,
Xenbase Ginsberg,
Xenopus 5S gene transcription factor, TFIIIA: characterization of a cDNA clone and measurement of RNA levels throughout development.
1984,
Pubmed
,
Xenbase Hanahan,
Plasmid screening at high colony density.
1980,
Pubmed Hanahan,
Studies on transformation of Escherichia coli with plasmids.
1983,
Pubmed Hunkapiller,
High-sensitivity sequencing with a gas-phase sequenator.
1983,
Pubmed Hunkapiller,
Isolation of microgram quantities of proteins from polyacrylamide gels for amino acid sequence analysis.
1983,
Pubmed Krotoski,
Developmental mutants isolated from wild-caught Xenopus laevis by gynogenesis and inbreeding.
1985,
Pubmed
,
Xenbase Melton,
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
1984,
Pubmed Miller,
Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes.
1985,
Pubmed
,
Xenbase Pelham,
Related 5S RNA transcription factors in Xenopus oocytes and somatic cells.
1981,
Pubmed
,
Xenbase Pelham,
A specific transcription factor that can bind either the 5S RNA gene or 5S RNA.
1980,
Pubmed
,
Xenbase Picard,
Isolation of a 7S particle from Xenopus laevis oocytes: a 5S RNA-protein complex.
1979,
Pubmed
,
Xenbase Shastry,
Altered levels of a 5 S gene-specific transcription factor (TFIIIA) during oogenesis and embryonic development of Xenopus laevis.
1984,
Pubmed
,
Xenbase Smith,
Domains of the positive transcription factor specific for the Xenopus 5S RNA gene.
1984,
Pubmed
,
Xenbase Thomas,
Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
1980,
Pubmed Wakefield,
Cytoplasmic regulation of 5S RNA genes in nuclear-transplant embryos.
1983,
Pubmed
,
Xenbase Wickens,
Synthesis of double-stranded DNA complementary to lysozyme, ovomucoid, and ovalbumin mRNAs. Optimization for full length second strand synthesis by Escherichia coli DNA polymerase I.
1978,
Pubmed Wormington,
Onset of 5 S RNA gene regulation during Xenopus embryogenesis.
1983,
Pubmed
,
Xenbase
