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To study the transcriptional regulation of genes during early Xenopus development, we have isolated a gene that is first transcribed at the mid-blastula transition. Transcription of this gene, called GS17, stops at mid-gastrula and the mRNA is rapidly degraded. Consequently, transcripts of GS17 are only present for a brief period, primarily during gastrulation. When the GS17 gene is injected into fertilized eggs, transcription from the injected DNA mimics the expression pattern of the endogenous gene, i.e., both the switch-on and switch-off of transcription are correctly regulated. The injected DNA is not significantly amplified and remains extrachromosomal. The correct expression of genes injected into Xenopus eggs will make it possible to investigate maternal factors involved in activating the embryonic genome.
Bendig,
Persistence and expression of histone genes injected into Xenopus eggs in early development.
1981, Pubmed,
Xenbase
Bendig,
Persistence and expression of histone genes injected into Xenopus eggs in early development.
1981,
Pubmed
,
Xenbase Bendig,
Replication and expression of Xenopus laevis globin genes injected into fertilized Xenopus eggs.
1983,
Pubmed
,
Xenbase Bendig,
Differential expression of the Xenopus laevis tadpole and adult beta-globin genes when injected into fertilized Xenopus laevis eggs.
1984,
Pubmed
,
Xenbase Benton,
Screening lambdagt recombinant clones by hybridization to single plaques in situ.
1977,
Pubmed BROWN,
RNA SYNTHESIS DURING THE DEVELOPMENT OF XENOPUS LAEVIS, THE SOUTH AFRICAN CLAWED TOAD.
1964,
Pubmed
,
Xenbase Busby,
Spacer sequences regulate transcription of ribosomal gene plasmids injected into Xenopus embryos.
1983,
Pubmed
,
Xenbase Chase,
Biogenesis of mitochondria during Xenopus laevis development.
1972,
Pubmed
,
Xenbase Colot,
Behavior of individual maternal pA+ RNAs during embryogenesis of Xenopus laevis.
1982,
Pubmed
,
Xenbase Etkin,
Replication, integration and expression of exogenous DNA injected into fertilized eggs of Xenopus laevis.
1984,
Pubmed
,
Xenbase Etkin,
Transformed Xenopus embryos as a transient expression system to analyze gene expression at the midblastula transition.
1985,
Pubmed
,
Xenbase Frischauf,
Lambda replacement vectors carrying polylinker sequences.
1983,
Pubmed Goldberg,
Correct developmental expression of a cloned alcohol dehydrogenase gene transduced into the Drosophila germ line.
1983,
Pubmed Gurdon,
Injected nuclei in frog oocytes: fate, enlargement, and chromatin dispersal.
1976,
Pubmed
,
Xenbase Gurdon,
The translation of mammalian globin mRNA injected into fertilized eggs of Xenopus laevis I. Message stability in development.
1974,
Pubmed
,
Xenbase Harland,
Regulated replication of DNA microinjected into eggs of Xenopus laevis.
1980,
Pubmed
,
Xenbase Krumlauf,
Developmental regulation of alpha-fetoprotein genes in transgenic mice.
1985,
Pubmed McGinnis,
A conserved DNA sequence in homoeotic genes of the Drosophila Antennapedia and bithorax complexes.
,
Pubmed Melton,
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
1984,
Pubmed Messing,
A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
1982,
Pubmed Mohun,
Cell type-specific activation of actin genes in the early amphibian embryo.
,
Pubmed
,
Xenbase Newport,
A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage.
1982,
Pubmed
,
Xenbase Newport,
A major developmental transition in early Xenopus embryos: II. Control of the onset of transcription.
1982,
Pubmed
,
Xenbase Palmiter,
Transgenic mice.
1985,
Pubmed Patient,
Internal organization of the major adult alpha- and beta-globin genes of X. laevis.
1980,
Pubmed
,
Xenbase Rastl,
Expression of the mitochondrial genome in Xenopus laevis: a map of transcripts.
1979,
Pubmed
,
Xenbase Rebagliati,
Identification and cloning of localized maternal RNAs from Xenopus eggs.
1985,
Pubmed
,
Xenbase Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed Sargent,
Differential gene expression in the gastrula of Xenopus laevis.
1983,
Pubmed
,
Xenbase Schwarzbauer,
Three different fibronectin mRNAs arise by alternative splicing within the coding region.
1983,
Pubmed Spradling,
The effect of chromosomal position on the expression of the Drosophila xanthine dehydrogenase gene.
1983,
Pubmed Wickens,
Post-transcriptional processing of simian virus 40 late transcripts in injected frog oocytes.
1983,
Pubmed
,
Xenbase Young,
Synthesis of mitochondrial RNA in disaggregated embryos of Xenopus laevis.
1973,
Pubmed
,
Xenbase Zinn,
Identification of two distinct regulatory regions adjacent to the human beta-interferon gene.
1983,
Pubmed
