Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
The effect of capping and polyadenylation on the stability, movement and translation of synthetic messenger RNAs in Xenopus oocytes.
Drummond DR, Armstrong J, Colman A.
???displayArticle.abstract???
Synthetic RNAs coding for chicken lysozyme, calf preprochymosin and Xenopus globin were transcribed in vitro using Sp6 RNA polymerase. The effects of capping and adding a poly(A) tail on the stability, movement and translation of these RNAs in Xenopus oocytes was examined. Capping and polyadenylation increased stability of the transcripts, with at least 40% remaining intact 48 h after injection into oocytes. Capped poly(A)- transcripts moved more rapidly in oocytes than either capped poly(A)+ transcripts or naturally occurring mRNAs. The translational efficiency of most of the synthetic RNAs in oocytes increased with both capping and polyadenylation. The exception was one Xenopus globin transcript which had an unusual 3' end of 20As and 30Cs, where further polyadenylation decreased translational efficiency. Polyadenylation was essential for detectable expression of the synthetic RNAs in cultured cells, but decreased translation of the synthetic RNAs in vitro.
Allende,
The degradation of ribonucleic acids injected into Xenopus laevis oocytes.
1974, Pubmed,
Xenbase
Allende,
The degradation of ribonucleic acids injected into Xenopus laevis oocytes.
1974,
Pubmed
,
Xenbase Ash,
Antibody-induced linkages of plasma membrane proteins to intracellular actomyosin-containing filaments in cultured fibroblasts.
1977,
Pubmed Banerjee,
5'-terminal cap structure in eucaryotic messenger ribonucleic acids.
1980,
Pubmed Berridge,
Translation of Xenopus liver messenger RNA in Xenopus oocytes: vitellogenin synthesis and conversion to yolk platelet proteins.
1976,
Pubmed
,
Xenbase Bonner,
A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
1974,
Pubmed Both,
Ribosome binding to reovirus mRNA in protein synthesis requires 5' terminal 7-methylguanosine.
1975,
Pubmed Capco,
Transient localizations of messenger RNA in Xenopus laevis oocytes.
1982,
Pubmed
,
Xenbase Cashdollar,
Molecular cloning of the complete genome of reovirus serotype 3.
1984,
Pubmed Cashdollar,
Cloning the double-stranded RNA genes of reovirus: sequence of the cloned S2 gene.
1982,
Pubmed Church,
Genomic sequencing.
1984,
Pubmed Cox,
Detection of mrnas in sea urchin embryos by in situ hybridization using asymmetric RNA probes.
1984,
Pubmed Davanloo,
Cloning and expression of the gene for bacteriophage T7 RNA polymerase.
1984,
Pubmed Deshpande,
Translation and stability of rat liver messenger RNA for alpha 2 mu-globulin in Xenopus oocyte. The role of terminal poly(A).
1979,
Pubmed
,
Xenbase Doel,
The translational capacity of deadenylated ovalbumin messenger RNA.
1976,
Pubmed Drummond,
Stability and movement of mRNAs and their encoded proteins in Xenopus oocytes.
1985,
Pubmed
,
Xenbase Furuichi,
5'-Terminal structure and mRNA stability.
1977,
Pubmed
,
Xenbase Graessmann,
Microinjection of early SV40 DNA fragments and T antigen.
1980,
Pubmed Green,
Human beta-globin pre-mRNA synthesized in vitro is accurately spliced in Xenopus oocyte nuclei.
1983,
Pubmed
,
Xenbase Gurdon,
Gene transfer in amphibian eggs and oocytes.
1981,
Pubmed Harris,
Molecular cloning and nucleotide sequence of cDNA coding for calf preprochymosin.
1982,
Pubmed Huez,
Functional stabilisation of HeLa cell histone messenger RNAs injected into Xenopus oocytes by 3'-OH polyadenylation.
1978,
Pubmed
,
Xenbase Huez,
Translational stability of native and deadenylylated rabbit globin mRNA injected into HeLa cells.
1981,
Pubmed
,
Xenbase Huez,
Role of the polyadenylate segment in the translation of globin messenger RNA in Xenopus oocytes.
1974,
Pubmed
,
Xenbase Huez,
Degradation of deadenylated rabbit alpha-globin mRNA in Xenopus oocytes is associated with its translation.
1977,
Pubmed
,
Xenbase Jacobson,
Possible involvement of poly(A) in protein synthesis.
1983,
Pubmed Jung,
Exons encode functional and structural units of chicken lysozyme.
1980,
Pubmed Konarska,
Recognition of cap structure in splicing in vitro of mRNA precursors.
1984,
Pubmed Krainer,
Normal and mutant human beta-globin pre-mRNAs are faithfully and efficiently spliced in vitro.
1984,
Pubmed Krieg,
Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs.
1984,
Pubmed
,
Xenbase Krieg,
Formation of the 3' end of histone mRNA by post-transcriptional processing.
,
Pubmed
,
Xenbase Krieg,
Efficient expression of cloned complementary DNAs for secretory proteins after injection into Xenopus oocytes.
1984,
Pubmed
,
Xenbase Land,
5'-Terminal sequences of eucaryotic mRNA can be cloned with high efficiency.
1981,
Pubmed Littauer,
The regulatory function of poly(A) and adjacent 3' sequences in translated RNA.
1982,
Pubmed Lockard,
Requirement for 7-methylguanosine in translation of globin mRNA in vivo.
1978,
Pubmed
,
Xenbase Marbaix,
Absence of polyadenylate segment in globin messenger RNA accelerates its degradation in Xenopus oocytes.
1975,
Pubmed
,
Xenbase Maxwell,
Removal of RNase activity from DNase by affinity chromatography on agarose coupled aminophenylphosphoryl-uridine-2' (3')-phosphate.
1977,
Pubmed McCrae,
Stability of non-polyadenylated viral mRNAs injected into frog oocytes.
1981,
Pubmed
,
Xenbase Mead,
Single stranded DNA SP6 promoter plasmids for engineering mutant RNAs and proteins: synthesis of a 'stretched' preproparathyroid hormone.
1985,
Pubmed Melton,
Injected anti-sense RNAs specifically block messenger RNA translation in vivo.
1985,
Pubmed
,
Xenbase Melton,
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
1984,
Pubmed Mishina,
Location of functional regions of acetylcholine receptor alpha-subunit by site-directed mutagenesis.
,
Pubmed
,
Xenbase Nakazato,
Localization of the polyadenylate sequences in messenger ribonucleic acid and in the heterogeneous nuclear ribonucleic acid of HeLa cells.
1973,
Pubmed NULL,
Biomembranes. Part J: Membrane biogenesis: assembly and targeting (general methods, eukaryotes).
1983,
Pubmed Palatnik,
Translational control during early Dictyostelium development: possible involvement of poly(A) sequences.
1984,
Pubmed Pelletier,
Insertion mutagenesis to increase secondary structure within the 5' noncoding region of a eukaryotic mRNA reduces translational efficiency.
1985,
Pubmed Richardson,
Nucleotide sequence of reovirus genome segment S3, encoding non-structural protein sigma NS.
1983,
Pubmed Richter,
Differential capacity for translation and lack of competition between mRNAs that segregate to free and membrane-bound polysomes.
1981,
Pubmed
,
Xenbase Rosenberg,
Production of phenocopies by Krüppel antisense RNA injection into Drosophila embryos.
,
Pubmed Rosenthal,
Sequence-specific adenylations and deadenylations accompany changes in the translation of maternal messenger RNA after fertilization of Spisula oocytes.
1983,
Pubmed Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed Sehgal,
Does 3'-terminal poly(A) stabilize human fibroblast interferon mRNA in oocytes of Xenopus laevis?
1978,
Pubmed
,
Xenbase Shatkin,
mRNA cap binding proteins: essential factors for initiating translation.
1985,
Pubmed Sippel,
Purification and characterization of adenosine triphosphate: ribonucleic acid adenyltransferase from Escherichia coli.
1973,
Pubmed Soreq,
Translational activity and functional stability of human fibroblast beta 1 and beta 2 interferon mRNAs lacking 3'-terminal RNA sequences.
1981,
Pubmed
,
Xenbase Valle,
Post-translational fate of variant MOPC 315 lambda chains in Xenopus oocytes and mouse myeloma cells.
1983,
Pubmed
,
Xenbase Wood,
Expression of active human factor VIII from recombinant DNA clones.
,
Pubmed Zinn,
Identification of two distinct regulatory regions adjacent to the human beta-interferon gene.
1983,
Pubmed
