Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Visualization of RNA polymerase II ternary transcription complexes formed in vitro on a Xenopus laevis vitellogenin gene.
ten Heggeler B, Wahli W.
???displayArticle.abstract???
Stable ternary transcription complexes assembled in vitro, using a HeLa whole-cell extract, have been isolated and visualized by electron microscopy. The formation of these stable complexes on the DNA fragment used as template, the 5' end region of the Xenopus laevis vitellogenin gene B2, depends on factors present in the whole-cell extract, RNA polymerase II and at least two nucleotides. Interestingly, bending in the DNA fragment was frequently observed at the binding site of RNA polymerase II. Dinucleotides that can prime initiation within a short sequence of approximately 10 contiguous nucleotides centered around the initiation site used in vivo, also favour the formation of stable complexes. In addition, pre-initiation complexes were isolated and it was shown that factors in the extract involved in their formation are more abundant than the RNA polymerase II molecules available for binding. The possible implication of this observation relative to the in vivo situation is discussed.
Ackerman,
RNA polymerase II ternary transcription complexes generated in vitro.
1983, Pubmed
Ackerman,
RNA polymerase II ternary transcription complexes generated in vitro.
1983,
Pubmed Bordier,
Electron microscopic localization of the binding sites of Escherichia coli RNA polymerase in the early promoter region of T7 DNA.
1974,
Pubmed Brack,
Electron microscopic study of the repressor of bacteriophage lambda and its interaction with operator DNA.
1975,
Pubmed Bunick,
Mechanism of RNA polymerase II--specific initiation of transcription in vitro: ATP requirement and uncapped runoff transcripts.
1982,
Pubmed Coppola,
Purification and characterization of ternary complexes containing accurately initiated RNA polymerase II and less than 20 nucleotides of RNA.
1984,
Pubmed Davison,
Formation of stable preinitiation complexes between eukaryotic class B transcription factors and promoter sequences.
1983,
Pubmed de Crombrugghe,
Cyclic AMP receptor protein: role in transcription activation.
1984,
Pubmed Dubochet,
A new preparation method for dark-field electron microscopy of biomacromolecules.
1971,
Pubmed Dynan,
Isolation of transcription factors that discriminate between different promoters recognized by RNA polymerase II.
1983,
Pubmed Fire,
Interactions between RNA polymerase II, factors, and template leading to accurate transcription.
1984,
Pubmed Germond,
Vitellogenin B2 gene in Xenopus laevis: isolation, in vitro transcription and relation to other vitellogenin genes.
1983,
Pubmed
,
Xenbase Gronenborn,
Visualization of cAMP receptor protein-induced DNA kinking by electron microscopy.
1984,
Pubmed Learned,
In vitro transcription of human ribosomal RNA genes by RNA polymerase I.
1982,
Pubmed Manley,
DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract.
1980,
Pubmed Matsui,
Multiple factors required for accurate initiation of transcription by purified RNA polymerase II.
1980,
Pubmed Rosenberg,
Regulatory sequences involved in the promotion and termination of RNA transcription.
1979,
Pubmed Safer,
Isolation of stable preinitiation, initiation, and elongation complexes from RNA polymerase II-directed transcription.
1985,
Pubmed Samuels,
Separation and characterization of factors mediating accurate transcription by RNA polymerase II.
1982,
Pubmed Samuels,
Dinucleotide priming of transcription mediated by RNA polymerase II.
1984,
Pubmed Stüber,
Organization of transcriptional signals in plasmids pBR322 and pACYC184.
1981,
Pubmed Tolunay,
Isolation of an active transcription initiation complex from HeLa cell-free extract.
1984,
Pubmed Vollenweider,
Electron microscopic mapping of RNA polymerase binding to coliphage lambda DNA.
1978,
Pubmed von Hippel,
Protein-nucleic acid interactions in transcription: a molecular analysis.
1984,
Pubmed Wahli,
Vitellogenin genes A1 and B1 are linked in the Xenopus laevis genome.
1982,
Pubmed
,
Xenbase Walker,
Sequence homologies in the region preceding the transcription initiation site of the liver estrogen-responsive vitellogenin and apo-VLDLII genes.
1984,
Pubmed
,
Xenbase Walker,
Sequence homologies within the 5' end region of the estrogen-controlled vitellogenin gene in Xenopus and chicken.
1983,
Pubmed
,
Xenbase Weil,
Faithful transcription of eukaryotic genes by RNA polymerase III in systems reconstituted with purified DNA templates.
1979,
Pubmed
,
Xenbase Wilkinson,
Transcription of Xenopus ribosomal RNA genes by RNA polymerase I in vitro.
1982,
Pubmed
,
Xenbase Wu,
The locus of sequence-directed and protein-induced DNA bending.
,
Pubmed
