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The involvement of Ca ions in the mediation of muscarinic Cl- current responses in Xenopus oocytes was studied using the voltage-clamp technique and direct measurements of 45Ca efflux. The injection of Ca into the oocytes produced a dose-dependent transient inward (depolarizing) current carried by Cl. This current was occasionally followed by a second, long-lasting inward current. The muscarinic response was evoked by the application of acetylcholine (ACh). It consisted of a transient inward current response, and a long-lasting inward current response, both inward currents carried by Cl ions. Both responses were inhibited by intracellular injection of ethyleneglycol-bis-(beta-aminoethylether)N,N'-tetraacetic acid (EGTA), the long-lasting response being inhibited faster than the transient response. The calmodulin inhibitor, trifluoperazine, inhibited both the Cl-current responses to ACh and to Ca injection. ACh (10 microM) evoked a release of 45Ca from pre-loaded oocytes. This effect was inhibited by atropine (1 microM). In the absence of external Ca, the muscarinic transient and long-lasting responses were partially inhibited. The long-lasting response was more sensitive to the external Ca depletion than the transient response. Repetitive applications of ACh in the absence of external Ca resulted in a progressive decrease in the response amplitudes. Under these conditions, a temporary exposure to normal Ca solution ('Ca window') resulted in a partial recovery of the response amplitudes. The muscarinic inward current responses were not inhibited by nifedipine (20 microM). In the presence of a high external concentration of Mn ions ([Mn]o = 18 mM), the transient response was potentiated. Subsequent applications of ACh in high [Mn]o resulted in progressively decreasing responses. It is concluded that the muscarinic Cl responses in Xenopus oocytes are mediated by an increase in the intracellular free Ca activity, aiCa. Ca ions involved in the mediation of the muscarinic Cl current responses are released from cellular Ca stores. It is also proposed that the transient and long-lasting responses result from the release of Ca from two different stores.
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