Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
DG81 is a cDNA clone derived from a subtracted library containing those RNA molecules that are present in gastrulae but absent from eggs of the frog Xenopus laevis. DG RNAs (where DG indicates differentially expressed in gastrula) represent the products of new transcription activated in the embryo at the midblastula transition or shortly thereafter. DG81 RNA is first detected in middle to late gastrulae, peaks in abundance in early tadpoles, and declines to background levels by the end of metamorphosis. Sequence analysis of an almost full-length cDNA clone homologous to DG81 allows deduction of a protein sequence that shows extensive homology to known intermediate filament proteins, most notably to epidermal type I cytokeratins. Consequently, the protein encoded by DG81 has been named XK81, for Xenopus keratin 81. In concert with keratins analyzed previously, XK81 has a central coiled-coil alpha-helical domain of 312 amino acids, which accounts for most of the homology to other keratins. This rod-like region is flanked by more divergent domains of 73 amino acids at the NH2 terminus and 44 amino acids at the COOH terminus. XK81 provides an example of a cytokeratin whose expression is limited to pre-adult developmental stages. We suggest that XK81 functions specifically in the differentiation of the tadpoleepidermis.
Aviv,
Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
1972, Pubmed
Aviv,
Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
1972,
Pubmed Bailey,
Methylmercury as a reversible denaturing agent for agarose gel electrophoresis.
1976,
Pubmed Banks-Schlegel,
Keratin alterations during embryonic epidermal differentiation: a presage of adult epidermal maturation.
1982,
Pubmed Boros,
High-copy-number derivatives of the plasmid cloning vector pBR322.
1984,
Pubmed Chirgwin,
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
1979,
Pubmed Church,
Genomic sequencing.
1984,
Pubmed Davidson,
Genetic information in oocyte RNA.
1971,
Pubmed Dworkin,
The accumulation of prominent tadpole mRNAs occurs at the beginning of neurulation in Xenopus laevis embryos.
1984,
Pubmed
,
Xenbase Eichner,
Classification of epidermal keratins according to their immunoreactivity, isoelectric point, and mode of expression.
1984,
Pubmed Franz,
Intermediate-size filaments in a germ cell: Expression of cytokeratins in oocytes and eggs of the frog Xenopus.
1983,
Pubmed
,
Xenbase Geisler,
Neurofilament architecture combines structural principles of intermediate filaments with carboxy-terminal extensions increasing in size between triplet proteins.
1983,
Pubmed Geisler,
The amino acid sequence of chicken muscle desmin provides a common structural model for intermediate filament proteins.
1982,
Pubmed Godsave,
Intermediate filaments in the Xenopus oocyte: the appearance and distribution of cytokeratin-containing filaments.
1984,
Pubmed
,
Xenbase Hanukoglu,
The cDNA sequence of a human epidermal keratin: divergence of sequence but conservation of structure among intermediate filament proteins.
1982,
Pubmed Hoffmann,
Amino acid sequence of the carboxy-terminal part of an acidic type I cytokeratin of molecular weight 51 000 from Xenopus laevis epidermis as predicted from the cDNA sequence.
1984,
Pubmed
,
Xenbase Jorcano,
Cell type-specific expression of bovine keratin genes as demonstrated by the use of complementary DNA clones.
1984,
Pubmed Kim,
Tissue specificity of epithelial keratins: differential expression of mRNAs from two multigene families.
1983,
Pubmed Lazarides,
Intermediate filaments: a chemically heterogeneous, developmentally regulated class of proteins.
1982,
Pubmed Lipman,
Rapid and sensitive protein similarity searches.
1985,
Pubmed Maxam,
Sequencing end-labeled DNA with base-specific chemical cleavages.
1980,
Pubmed Mohun,
Cell type-specific activation of actin genes in the early amphibian embryo.
,
Pubmed
,
Xenbase Moll,
The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells.
1982,
Pubmed Newport,
A major developmental transition in early Xenopus embryos: II. Control of the onset of transcription.
1982,
Pubmed
,
Xenbase Newport,
A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage.
1982,
Pubmed
,
Xenbase Okayama,
A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells.
1983,
Pubmed Perlman,
Analysis of Xenopus laevis ovary and somatic cell polyadenylated RNA by molecular hybridization.
1978,
Pubmed
,
Xenbase Sagata,
A study on the steady-state population of poly(A)+RNA during early development of Xenopus laevis.
1980,
Pubmed
,
Xenbase Sargent,
Differential gene expression in the gastrula of Xenopus laevis.
1983,
Pubmed
,
Xenbase Steinert,
Complete amino acid sequence of a mouse epidermal keratin subunit and implications for the structure of intermediate filaments.
1983,
Pubmed Steinert,
The complete cDNA and deduced amino acid sequence of a type II mouse epidermal keratin of 60,000 Da: analysis of sequence differences between type I and type II keratins.
1984,
Pubmed
