Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
A protein kinase specific for ribosomal protein S6 has been purified from eggs of Xenopus laevis. As visualized on a silver-stained polyacrylamide gel, the major protein in the preparation migrated with a Mr of 90,000. Incubation of the enzyme preparation with [gamma-32P]ATP led to phosphorylation of this protein on serine residues. Upon glycerol gradient centrifugation, the S6 kinase activity and the Mr 90,000 protein both sedimented with a Mr of 50,000-55,000. Two-dimensional gel electrophoresis demonstrated that up to 4-5 phosphate groups per S6 molecule could be incorporated with this enzyme in vitro, and two-dimensional peptide mapping demonstrated that the phosphopeptides from S6 labeled in vitro with the enzyme comigrated with those from highly phosphorylated S6 labeled in vivo in response to progesterone treatment. The purified S6 protein kinase did not phosphorylate at a significant rate ribosomal protein S10, histone H1, histone H4, mixed histones, casein, or phosvitin, indicating a high degree of substrate specificity. These results indicate that activation of a single S6 protein kinase may be sufficient to account for increased S6 phosphorylation after a growth stimulus.
Beavo,
Preparation of homogeneous cyclic AMP-dependent protein kinase(s) and its subunits from rabbit skeletal muscle.
1974, Pubmed
Beavo,
Preparation of homogeneous cyclic AMP-dependent protein kinase(s) and its subunits from rabbit skeletal muscle.
1974,
Pubmed Blenis,
Phosphorylation of the ribosomal protein S6 is elevated in cells transformed by a variety of tumor viruses.
1984,
Pubmed Burkhard,
Changes in ribosome function by cAMP-dependent and cAMP-independent phosphorylation of ribosomal protein S6.
1983,
Pubmed Cobb,
Description of a protein kinase derived from insulin-treated 3T3-L1 cells that catalyzes the phosphorylation of ribosomal protein S6 and casein.
1983,
Pubmed Collett,
Structural analysis of the avian sarcoma virus transforming protein: sites of phosphorylation.
1979,
Pubmed Collett,
Avian sarcoma virus-transforming protein, pp60src shows protein kinase activity specific for tyrosine.
1980,
Pubmed Cooper,
Identification and characterization of cellular targets for tyrosine protein kinases.
1983,
Pubmed Cox,
Ribosomes from Xenopus laevis ovaries and the polyuridylic acid-directed biosynthesis of polyphenylalanine.
1970,
Pubmed
,
Xenbase Decker,
Phosphorylation of ribosomal protein S6 in avian sarcoma virus-transformed chicken embryo fibroblasts.
1981,
Pubmed Donahue,
Phosphorylation of ribosomal protein S6 at multiple sites by a cyclic AMP-independent protein kinase from lymphoid cells.
1984,
Pubmed Duncan,
Preferential utilization of phosphorylated 40-S ribosomal subunits during initiation complex formation.
1982,
Pubmed Ek,
Stimulation of tyrosine-specific phosphorylation by platelet-derived growth factor.
1982,
Pubmed Feldman,
Avian sarcoma virus UR2 encodes a transforming protein which is associated with a unique protein kinase activity.
1982,
Pubmed Feldman,
Characterization of protein kinase activity associated with the transforming gene product of Fujinami sarcoma virus.
1980,
Pubmed Flockhart,
Regulatory mechanisms in the control of protein kinases.
1982,
Pubmed Glover,
Heat shock induces rapid dephosphorylation of a ribosomal protein in Drosophila.
1982,
Pubmed Haselbacher,
Insulin-like growth factor: insulin or serum increase phosphorylation of ribosomal protein S6 during transition of stationary chick embryo fibroblasts into early G1 phase of the cell cycle.
1979,
Pubmed Hunter,
Transforming gene product of Rous sarcoma virus phosphorylates tyrosine.
1980,
Pubmed Jacobs,
Somatomedin-C stimulates the phosphorylation of the beta-subunit of its own receptor.
1983,
Pubmed Johnson,
The enzymatic preparation of [alpha-32P]ATP, [alpha-32P]GTP, [32P]cAMP, and [32P]cGMP, and their use in the assay of adenylate and guanylate cyclases and cyclic nucleotide phosphodiesterases.
1979,
Pubmed Kasuga,
Insulin stimulation of phosphorylation of the beta subunit of the insulin receptor. Formation of both phosphoserine and phosphotyrosine.
1982,
Pubmed Kasuga,
Insulin stimulates tyrosine phosphorylation of the insulin receptor in a cell-free system.
1982,
Pubmed Kawai,
Characterization of Y73, an avian sarcoma virus: a unique transforming gene and its product, a phosphopolyprotein with protein kinase activity.
1980,
Pubmed Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed Le Peuch,
Purified rat brain calcium- and phospholipid-dependent protein kinase phosphorylates ribosomal protein S6.
1983,
Pubmed Lubben,
Cyclic nucleotide-independent protein kinases from rabbit reticulocytes. Purification and characterization of protease-activated kinase II.
1983,
Pubmed Maller,
Changes in protein phosphorylation accompanying maturation of Xenopus laevis oocytes.
1977,
Pubmed
,
Xenbase Martin-Pérez,
Ordered phosphorylation of 40S ribosomal protein S6 after serum stimulation of quiescent 3T3 cells.
1983,
Pubmed Naharro,
Analysis of the primary translational product and integrated DNA of a new feline sarcoma virus, GR-FeSV.
1983,
Pubmed Neil,
Tyrosine-specific protein kinase activity associated with p105 of avian sarcoma virus PRCII.
1981,
Pubmed Nielsen,
Increased phosphorylation of ribosomal protein S6 during meiotic maturation of Xenopus oocytes.
1982,
Pubmed
,
Xenbase Nishimura,
Platelet-derived growth factor stimulates the phosphorylation of ribosomal protein S6.
1983,
Pubmed Novak-Hofer,
An activated S6 kinase in extracts from serum- and epidermal growth factor-stimulated Swiss 3T3 cells.
1984,
Pubmed Oakley,
A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels.
1980,
Pubmed Parker,
Glycogen synthase from rabbit skeletal muscle; effect of insulin on the state of phosphorylation of the seven phosphoserine residues in vivo.
1983,
Pubmed Perisic,
Protease-activated kinase II as the potential mediator of insulin-stimulated phosphorylation of ribosomal protein S6.
1983,
Pubmed Richter,
The mechanism for increased protein synthesis during Xenopus oocyte maturation.
1982,
Pubmed
,
Xenbase Rubin,
Stimulation of tyrosine-specific phosphorylation in vitro by insulin-like growth factor I.
,
Pubmed Scott,
Translational control in lysates of Drosophila melanogaster cells.
1981,
Pubmed Spivack,
Microinjection of pp60v-src into Xenopus oocytes increases phosphorylation of ribosomal protein S6 and accelerates the rate of progesterone-induced meiotic maturation.
1984,
Pubmed
,
Xenbase Stith,
The effect of insulin on intracellular ph and ribosomal protein S6 phosphorylation in oocytes of Xenopus laevis.
1984,
Pubmed
,
Xenbase Swergold,
Hormonal regulation of the phosphorylation of ATP citrate lyase in 3T3-L1 adipocytes. Effects of insulin and isoproterenol.
1982,
Pubmed Thomas,
Transcriptional and translational control of cytoplasmic proteins after serum stimulation of quiescent Swiss 3T3 cells.
1981,
Pubmed Thomas,
The effect of serum, EGF, PGF2 alpha and insulin on S6 phosphorylation and the initiation of protein and DNA synthesis.
1982,
Pubmed Ushiro,
Identification of phosphotyrosine as a product of epidermal growth factor-activated protein kinase in A-431 cell membranes.
1980,
Pubmed Walsh,
Krebs EG: Purification and characterization of a protein inhibitor of adenosine 3',5'-monophosphate-dependent protein kinases.
1971,
Pubmed Wang,
Expression of an Abelson murine leukemia virus-encoded protein in Escherichia coli causes extensive phosphorylation of tyrosine residues.
1982,
Pubmed Wettenhall,
Isolation and characterisation of cyclic AMP-dependent phosphorylation sites from rat liver ribosomal protein S6.
1982,
Pubmed Witte,
Abelson murine leukaemia virus protein is phosphorylated in vitro to form phosphotyrosine.
1980,
Pubmed Wu,
Partial purification and characterization of the maturation-promoting factor from eggs of Xenopus laevis.
1980,
Pubmed
,
Xenbase Zasloff,
Purification of eukaryotic initiation factor 1 (EIF1) from Artemia salina embryos.
1974,
Pubmed
