Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Cell Biol
1985 Feb 01;1002:574-87. doi: 10.1083/jcb.100.2.574.
Show Gene links
Show Anatomy links
Membrane morphogenesis in retinal rod outer segments: inhibition by tunicamycin.
Fliesler SJ, Rayborn ME, Hollyfield JG.
???displayArticle.abstract???
Isolated Xenopus laevis retinas were incubated with 3H-labeled mannose or leucine in the presence or absence of tunicamycin (TM), a selective inhibitor of dolichyl phosphate-dependent protein glycosylation. At a TM concentration of 20 micrograms/ml, the incorporation of [3H]mannose and [3H]leucine into retinal macromolecules was inhibited by approximately 66 and 12-16%, respectively, relative to controls. Cellular uptake of the radiolabeled substrates was not inhibited at this TM concentration. Polyacrylamide gel electrophoresis revealed that TM had little effect on the incorporation of [3H]leucine into the proteins of whole retinas and that labeling of proteins (especially opsin) in isolated rod outer segment (ROS) membranes was negligible. The incorporation of [3H]mannose into proteins of whole retinas and ROS membranes was nearly abolished in the presence of TM. Autoradiograms of control retinas incubated with either [3H]mannose or [3H]leucine exhibited a discrete concentration of silver grains over ROS basal disc membranes. In TM-treated retinas, the extracellular space between rod inner and outer segments was dilated and filled with numerous heterogeneously size vesicles, which were labeled with [3H]leucine but not with [3H]mannose. ROS disc membranes per se were not labeled in the TM-treated retinas. Quantitative light microscopic autoradiography of retinas pulse-labeled with [3H]leucine showed no differences in labeling of rod cellular compartments in the presence or absence of TM as a function of increasing chase time. These results demonstrate that TM can block retinal protein glycosylation and normal disc membrane assembly under conditions where synthesis and intracellular transport of rod cell proteins (e.g., opsin) are not inhibited.
Ashwell,
Carbohydrate-specific receptors of the liver.
1982, Pubmed
Ashwell,
Carbohydrate-specific receptors of the liver.
1982,
Pubmed Barondes,
Soluble lectins: a new class of extracellular proteins.
1984,
Pubmed Basinger,
Rhodopsin biosynthesis in vitro.
1973,
Pubmed Bernard,
Carbohydrates selectively protect a specific domain of fibronectin against proteases.
1982,
Pubmed Besharse,
Membrane assembly in retinal photoreceptors I. Freeze-fracture analysis of cytoplasmic vesicles in relationship to disc assembly.
1980,
Pubmed
,
Xenbase Besharse,
Photoreceptor outer segments: accelerated membrane renewal in rods after exposure to light.
1977,
Pubmed
,
Xenbase Bok,
Proceedings: Autoradiographic and radiobiochemical studies on the incorporation of (6-3H)glucosamine into frog rhodopsin.
1974,
Pubmed Duksin,
Relationship of the structure and biological activity of the natural homologues of tunicamycin.
1982,
Pubmed Fliesler,
Glycoprotein synthesis in the human retina: localization of the lipid intermediate pathway.
1984,
Pubmed Fukuda,
Rhodopsin carbohydrate. Structure of small oligosaccharides attached at two sites near the NH2 terminus.
1979,
Pubmed Goldman,
In vitro biosynthesis, core glycosylation, and membrane integration of opsin.
1981,
Pubmed Hall,
Biosynthesis and assembly of the rod outer segment membrane system. Formation and fate of visual pigment in the frog retina.
1969,
Pubmed Heath,
Simple sugars inhibit rod outer segment disc shedding by the frog retina.
1983,
Pubmed Heller,
Structure of the glycopeptide from bovine visual pigment 500.
1970,
Pubmed Heller,
Structure of visual pigments. I. Purification, molecular weight, and composition of bovine visual pigment500.
1968,
Pubmed Hollyfield,
Turnover of rod photoreceptor outer segments. I. Membrane addition and loss in relationship to temperature.
1977,
Pubmed Hollyfield,
The emergence, localization and maturation of neurotransmitter systems during development of the retina in Xenopus laevis. I. Gamma aminobutyric acid.
1979,
Pubmed
,
Xenbase Hollyfield,
Membrane addition to rod photoreceptor outer segments: light stimulates membrane assembly in the absence of increased membrane biosynthesis.
1982,
Pubmed
,
Xenbase Kinney,
The photoreceptors and pigment epithelium of the larval Xenopus retina: morphogenesis and outer segment renewal.
1978,
Pubmed
,
Xenbase Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed Lehle,
The specific site of tunicamycin inhibition in the formation of dolichol-bound N-acetylglucosamine derivatives.
1976,
Pubmed Liang,
Structure of the carbohydrate moieties of bovine rhodopsin.
1979,
Pubmed Marshall,
Some observations on why many proteins are glycosylated.
1979,
Pubmed Matheke,
The effects of monensin on transport of membrane components in the frog retinal photoreceptor. I. Light microscopic autoradiography and biochemical analysis.
1984,
Pubmed Montreuil,
Primary structure of glycoprotein glycans: basis for the molecular biology of glycoproteins.
1980,
Pubmed O'Brien,
Differential effects of puromycin on the incorporation of precursors of rhodopsin in bovine retina.
1977,
Pubmed O'Brien,
Rhodopsin as a glycoprotein: a possible role for the oligosaccharide in phagocytosis.
1976,
Pubmed O'Brien,
Proceedings: The biosynthesis of rhodopsin in vitro.
1974,
Pubmed O'Brien,
Synthesis of rhodopsin and opsin in vitro.
1975,
Pubmed O'Brien,
Incorporation of mannose into rhodopsin in isolated bovine retina.
1977,
Pubmed Olden,
Carbohydrate moieties of glycoproteins. A re-evaluation of their function.
1982,
Pubmed Olden,
Function of the carbohydrate moieties of glycoproteins.
1982,
Pubmed Papermaster,
Rhodopsin content in the outer segment membranes of bovine and frog retinal rods.
1974,
Pubmed Papermaster,
Immunocytochemical localization of opsin in outer segments and Golgi zones of frog photoreceptor cells. An electron microscope analysis of cross-linked albumin-embedded retinas.
1978,
Pubmed Peters,
Fine structure of a periciliary ridge complex of frog retinal rod cells revealed by ultrahigh resolution scanning electron microscopy.
1983,
Pubmed Plantner,
Effect of tunicamycin on the glycosylation of rhodopsin.
1980,
Pubmed Plantner,
Carbohydrate composition of bovine rhodopsin.
1976,
Pubmed Pratt,
Tunicamycin-induced alterations in the synthesis of sulfated proteoglycans and cell surface morphology in the chick embryo fibroblast.
1979,
Pubmed Schwarz,
The lipid pathway of protein glycosylation and its inhibitors: the biological significance of protein-bound carbohydrates.
1982,
Pubmed Steinberg,
Disc morphogenesis in vertebrate photoreceptors.
1980,
Pubmed Struck,
Evidence for the participation of saccharide-lipids in the synthesis of the oligosaccharide chain of ovalbumin.
1977,
Pubmed Tkacz,
Tunicamycin inhibition of polyisoprenyl N-acetylglucosaminyl pyrophosphate formation in calf-liver microsomes.
1975,
Pubmed Udenfriend,
Fluorescamine: a reagent for assay of amino acids, peptides, proteins, and primary amines in the picomole range.
1972,
Pubmed Young,
The renewal of photoreceptor cell outer segments.
1967,
Pubmed Young,
Visual cells and the concept of renewal.
1976,
Pubmed
