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Phosphorylation of ribosomal protein S6 on serine after microinjection of the Abelson murine leukemia virus tyrosine-specific protein kinase into Xenopus oocytes.
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Phosphorylation of ribosomal protein S6 in NIH 3T3 fibroblasts is dependent on the presence of serum, but after transformation of these cells by Abelson murine leukemia virus (Ab-MuLV), S6 remained highly phosphorylated on serine residues either in the absence or the presence of serum. To investigate whether S6 phosphorylation in this system was a consequence of the action of the Ab-MuLV tyrosine-specific protein kinase, purified Ab-MuLV kinase made in Escherichia coli was microinjected into Xenopus oocytes and was observed to cause a 7- to 15-fold increase in the phosphorylation of S6 on serine residues. Two-dimensional phosphopeptide maps of S6 phosphorylated in Ab-MuLV-transformed NIH cells in the absence of serum were identical to those of S6 isolated from normal cells grown in the presence of serum. In addition, S6 from oocytes injected with Ab-MuLV kinase yielded an S6 phosphopeptide map indistinguishable from that of serum-stimulated NIH 3T3 cells, whereas S6 from control oocytes lacked several phosphopeptides. Ab-MuLV kinase did not phosphorylate S6 directly in vitro, and microinjection of a mutant Ab-MuLV protein lacking kinase activity had no effect. These results indicate that the Ab-MuLV kinase interacts with a cellular pathway to enhance S6 phosphorylation by directly or indirectly activating an S6 protein kinase and/or inactivating an S6 protein phosphatase.
Ballinger,
Fertilization of sea urchin eggs is accompanied by 40 S ribosomal subunit phosphorylation.
1981, Pubmed
Ballinger,
Fertilization of sea urchin eggs is accompanied by 40 S ribosomal subunit phosphorylation.
1981,
Pubmed Blenis,
Phorbol ester, serum, and rous sarcoma virus transforming gene product induce similar phosphorylations of ribosomal protein S6.
1984,
Pubmed Burkhard,
Changes in ribosome function by cAMP-dependent and cAMP-independent phosphorylation of ribosomal protein S6.
1983,
Pubmed Cobb,
Description of a protein kinase derived from insulin-treated 3T3-L1 cells that catalyzes the phosphorylation of ribosomal protein S6 and casein.
1983,
Pubmed Collett,
Avian sarcoma virus-transforming protein, pp60src shows protein kinase activity specific for tyrosine.
1980,
Pubmed Cooper,
Identification and characterization of cellular targets for tyrosine protein kinases.
1983,
Pubmed Decker,
Phosphorylation of ribosomal protein S6 in avian sarcoma virus-transformed chicken embryo fibroblasts.
1981,
Pubmed Donahue,
Phosphorylation of ribosomal protein S6 at multiple sites by a cyclic AMP-independent protein kinase from lymphoid cells.
1984,
Pubmed Duncan,
Preferential utilization of phosphorylated 40-S ribosomal subunits during initiation complex formation.
1982,
Pubmed Ek,
Stimulation of tyrosine-specific phosphorylation by platelet-derived growth factor.
1982,
Pubmed Feldman,
Avian sarcoma virus UR2 encodes a transforming protein which is associated with a unique protein kinase activity.
1982,
Pubmed Feldman,
Characterization of protein kinase activity associated with the transforming gene product of Fujinami sarcoma virus.
1980,
Pubmed Frackelton,
Characterization and use of monoclonal antibodies for isolation of phosphotyrosyl proteins from retrovirus-transformed cells and growth factor-stimulated cells.
1983,
Pubmed Glover,
Heat shock induces rapid dephosphorylation of a ribosomal protein in Drosophila.
1982,
Pubmed Gressner,
The phosphorylation of liver ribosomal proteins in vivo. Evidence that only a single small subunit protein (S6) is phosphorylated.
1974,
Pubmed Haselbacher,
Insulin-like growth factor: insulin or serum increase phosphorylation of ribosomal protein S6 during transition of stationary chick embryo fibroblasts into early G1 phase of the cell cycle.
1979,
Pubmed Hofmann,
Concentrations of cyclic AMP-dependent protein kinase subunits in various tissues.
1977,
Pubmed Hunter,
Transforming gene product of Rous sarcoma virus phosphorylates tyrosine.
1980,
Pubmed Jacobs,
Somatomedin-C stimulates the phosphorylation of the beta-subunit of its own receptor.
1983,
Pubmed Kasuga,
Insulin stimulates tyrosine phosphorylation of the insulin receptor in a cell-free system.
1982,
Pubmed Kasuga,
Insulin stimulation of phosphorylation of the beta subunit of the insulin receptor. Formation of both phosphoserine and phosphotyrosine.
1982,
Pubmed Kawai,
Characterization of Y73, an avian sarcoma virus: a unique transforming gene and its product, a phosphopolyprotein with protein kinase activity.
1980,
Pubmed Le Peuch,
Purified rat brain calcium- and phospholipid-dependent protein kinase phosphorylates ribosomal protein S6.
1983,
Pubmed Maller,
Use of microinjection techniques to study protein kinases and protein phosphorylation in amphibian oocytes.
1983,
Pubmed
,
Xenbase Martin-Pérez,
EGF, PGF2 alpha and insulin induce the phosphorylation of identical S6 peptides in swiss mouse 3T3 cells: effect of cAMP on early sites of phosphorylation.
1984,
Pubmed Naharro,
Analysis of the primary translational product and integrated DNA of a new feline sarcoma virus, GR-FeSV.
1983,
Pubmed Neil,
Tyrosine-specific protein kinase activity associated with p105 of avian sarcoma virus PRCII.
1981,
Pubmed Nielsen,
Increased phosphorylation of ribosomal protein S6 during meiotic maturation of Xenopus oocytes.
1982,
Pubmed
,
Xenbase Nishimura,
Platelet-derived growth factor stimulates the phosphorylation of ribosomal protein S6.
1983,
Pubmed Nishimura,
Stimulation of protein phosphorylation in Swiss mouse 3T3 cells by human platelet derived growth factor.
1981,
Pubmed Nishizuka,
The role of protein kinase C in cell surface signal transduction and tumour promotion.
,
Pubmed Novak-Hofer,
An activated S6 kinase in extracts from serum- and epidermal growth factor-stimulated Swiss 3T3 cells.
1984,
Pubmed Parker,
Glycogen synthase from rabbit skeletal muscle; effect of insulin on the state of phosphorylation of the seven phosphoserine residues in vivo.
1983,
Pubmed Perisic,
Protease-activated kinase II as the potential mediator of insulin-stimulated phosphorylation of ribosomal protein S6.
1983,
Pubmed Prywes,
Sequences of the A-MuLV protein needed for fibroblast and lymphoid cell transformation.
1983,
Pubmed Queen,
A vector that uses phage signals for efficient synthesis of proteins in Escherichia coli.
1983,
Pubmed Rubin,
Stimulation of tyrosine-specific phosphorylation in vitro by insulin-like growth factor I.
,
Pubmed Scott,
Translational control in lysates of Drosophila melanogaster cells.
1981,
Pubmed Spivack,
Microinjection of pp60v-src into Xenopus oocytes increases phosphorylation of ribosomal protein S6 and accelerates the rate of progesterone-induced meiotic maturation.
1984,
Pubmed
,
Xenbase Stith,
The effect of insulin on intracellular ph and ribosomal protein S6 phosphorylation in oocytes of Xenopus laevis.
1984,
Pubmed
,
Xenbase Swergold,
Hormonal regulation of the phosphorylation of ATP citrate lyase in 3T3-L1 adipocytes. Effects of insulin and isoproterenol.
1982,
Pubmed Terao,
Proteins of small subunits of rat liver ribosomes that interact with poly(U). II. Cross-links between poly(U) and ribosomal proteins in 40 S subunits induced by UV irradiation.
1979,
Pubmed Thomas,
The effect of serum, EGF, PGF2 alpha and insulin on S6 phosphorylation and the initiation of protein and DNA synthesis.
1982,
Pubmed Trevillyan,
Tumor-promoting phorbol esters stimulate the phosphorylation of ribosomal protein S6 in quiescent Reuber H35 hepatoma cells.
1984,
Pubmed Ushiro,
Identification of phosphotyrosine as a product of epidermal growth factor-activated protein kinase in A-431 cell membranes.
1980,
Pubmed Wang,
Expression of an Abelson murine leukemia virus-encoded protein in Escherichia coli causes extensive phosphorylation of tyrosine residues.
1982,
Pubmed Wettenhall,
Differential phosphorylation of ribosomal protein S6 in isolated rat hepatocytes after incubation with insulin and glucagon.
1982,
Pubmed Witte,
Abelson murine leukaemia virus protein is phosphorylated in vitro to form phosphotyrosine.
1980,
Pubmed
