Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
1984 Jun 01;36:1301-6. doi: 10.1002/j.1460-2075.1984.tb01966.x.
Show Gene links
Show Anatomy links
Amino acid sequence of the carboxy-terminal part of an acidic type I cytokeratin of molecular weight 51 000 from Xenopus laevis epidermis as predicted from the cDNA sequence.
???displayArticle.abstract???
The DNA sequence of a clone from a cDNA library made from Xenopus laevis skin is described. This sequence represents the 3'-terminal end of an mRNA which codes for an epidermal cytokeratin polypeptide of mol. wt. 51 000 of the acidic (type I) subfamily as identified by hybridization-selection of mRNAs, followed by gel electrophoretic identification of the polypeptides synthesized by translation in vitro. The partial amino acid sequence of the amphibian cytokeratin shows strong similarity to type I cytoskeletal keratins from human (mol. wt. 50 000) and murine (mol. wt. 59 000) epidermis. In the non alpha-helical tail region the human and the non-mammalian (Xenopus) keratins are more similar to each other than to the murine protein, indicating that the former are equivalent cytokeratin polypeptides and belonging to a special subclass of type I keratin polypeptides devoid of glycine-rich regions in the carboxy-terminal portion. The evolutionary conservativity of the genes coding for cytokeratins is discussed.
Anastasi,
Presence of caerulein in extracts of the skin of Leptodactylus pentadactylus labyrinthicus and of Xenopus laevis.
1970, Pubmed,
Xenbase
Anastasi,
Presence of caerulein in extracts of the skin of Leptodactylus pentadactylus labyrinthicus and of Xenopus laevis.
1970,
Pubmed
,
Xenbase Chou,
Prediction of beta-turns.
1979,
Pubmed Chou,
Empirical predictions of protein conformation.
1978,
Pubmed Franke,
Diversity of cytokeratins. Differentiation specific expression of cytokeratin polypeptides in epithelial cells and tissues.
1981,
Pubmed Franke,
Significance of two desmosome plaque-associated polypeptides of molecular weights 75 000 and 83 000.
1983,
Pubmed Franke,
Protein complexes of intermediate-sized filaments: melting of cytokeratin complexes in urea reveals different polypeptide separation characteristics.
1983,
Pubmed Franz,
Intermediate-size filaments in a germ cell: Expression of cytokeratins in oocytes and eggs of the frog Xenopus.
1983,
Pubmed
,
Xenbase Freudenstein,
Reaction of tonofilament-like intermediate-sized filaments with antibodies raised against isolated defined polypeptides of bovine hoof prekeratin.
1978,
Pubmed
,
Xenbase Fuchs,
Multiple keratins of cultured human epidermal cells are translated from different mRNA molecules.
1979,
Pubmed Fuchs,
Two distinct classes of keratin genes and their evolutionary significance.
1981,
Pubmed Fuchs,
Type I and type II keratins have evolved from lower eukaryotes to form the epidermal intermediate filaments in mammalian skin.
1983,
Pubmed Geisler,
The amino acid sequence of chicken muscle desmin provides a common structural model for intermediate filament proteins.
1982,
Pubmed Geisler,
Amino acid sequence data on glial fibrillary acidic protein (GFA); implications for the subdivision of intermediate filaments into epithelial and non-epithelial members.
1983,
Pubmed Gibbs,
Epidermal keratin messenger RNAs: a heterogeneous family.
1982,
Pubmed Gigi,
Detection of a cytokeratin determinant common to diverse epithelial cells by a broadly cross-reacting monoclonal antibody.
1982,
Pubmed Hanukoglu,
The cDNA sequence of a Type II cytoskeletal keratin reveals constant and variable structural domains among keratins.
1983,
Pubmed Hanukoglu,
The cDNA sequence of a human epidermal keratin: divergence of sequence but conservation of structure among intermediate filament proteins.
1982,
Pubmed Hoffmann,
Biosynthesis of caerulein in the skin of Xenopus laevis: partial sequences of precursors as deduced from cDNA clones.
1983,
Pubmed
,
Xenbase Hoffmann,
A novel peptide designated PYLa and its precursor as predicted from cloned mRNA of Xenopus laevis skin.
1983,
Pubmed
,
Xenbase Kim,
Tissue specificity of epithelial keratins: differential expression of mRNAs from two multigene families.
1983,
Pubmed Knöchel,
Molecular cloning and sequencing of mRNAs coding for minor adult globin polypeptides of Xenopus laevis.
1983,
Pubmed
,
Xenbase Kreis,
De novo synthesis and specific assembly of keratin filaments in nonepithelial cells after microinjection of mRNA for epidermal keratin.
1983,
Pubmed Magin,
Translational products of mRNAs coding for non-epidermal cytokeratins.
1983,
Pubmed
,
Xenbase McLachlan,
Coiled coil formation and sequence regularities in the helical regions of alpha-keratin.
1978,
Pubmed McMaster,
Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.
1977,
Pubmed Moll,
The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells.
1982,
Pubmed Osterburg,
Computer programs for the analysis and the management of DNA sequences.
1982,
Pubmed Proudfoot,
3' non-coding region sequences in eukaryotic messenger RNA.
1976,
Pubmed Ricciardi,
Purification and mapping of specific mRNAs by hybridization-selection and cell-free translation.
1979,
Pubmed Roop,
Keratin gene expression in mouse epidermis and cultured epidermal cells.
1983,
Pubmed Schiller,
A subfamily of relatively large and basic cytokeratin polypeptides as defined by peptide mapping is represented by one or several polypeptides in epithelial cells.
1982,
Pubmed Schweizer,
Synthesis in vitro of keratin polypeptides directed by mRNA isolated from newborn and adult mouse epidermis.
1980,
Pubmed Steinert,
Complete amino acid sequence of a mouse epidermal keratin subunit and implications for the structure of intermediate filaments.
1983,
Pubmed Thomas,
Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
1980,
Pubmed Vieira,
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
1982,
Pubmed
