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Xenopus laevis U1 snRNA genes are found in several different genomic arrangements. The major family of genes is organised in tandem repeats of 1.8 kb. The minor U1-family is much less abundant and is present on 1.2-kb HinfI restriction fragments. In addition there are genomic arrangements present in one or very few copies, which could represent the ends of repeating units. There is no evidence for the presence of U1 pseudogenes in Xenopus. A cluster of U1 snRNA genes consisting of a member of the minor class of U1 snRNA genes and two of the 'rarely represented' genes was cloned. All three genes were expressed upon microinjection into frog oocytes. A fragment containing 149 bp of 5' flanking sequence, the RNA coding sequence, and 27 bp of 3' flanking sequence was shown to be accurately transcribed into U1 snRNA. These oocyte transcripts are assembled into specific U1 snRNPs. Sequence comparison of the regions flanking Xenopus U1 and U2 snRNA genes showed the presence of two blocks of homology, which are also conserved in many other U snRNA genes. One of these blocks is found at position -60 to -50 before the coding sequence, and we discuss its possible role in the correct initiation of transcription. The other is 3' to the coding sequence and may be involved in the accurate production of mature 3' ends in the RNA.
Benton,
Screening lambdagt recombinant clones by hybridization to single plaques in situ.
1977, Pubmed
Benton,
Screening lambdagt recombinant clones by hybridization to single plaques in situ.
1977,
Pubmed Bernstein,
Pseudogenes for human small nuclear RNA U3 appear to arise by integration of self-primed reverse transcripts of the RNA into new chromosomal sites.
1983,
Pubmed Branlant,
Nucleotide sequences of nuclear U1A RNAs from chicken, rat and man.
1980,
Pubmed Busch,
SnRNAs, SnRNPs, and RNA processing.
1982,
Pubmed Card,
Sea urchin small nuclear RNA genes are organized in distinct tandemly repeating units.
1982,
Pubmed Corden,
Promoter sequences of eukaryotic protein-coding genes.
1980,
Pubmed Cortese,
Transcription of tRNA genes in vivo: single-stranded compared to double-stranded templates.
1980,
Pubmed
,
Xenbase De Robertis,
Nucleocytoplasmic segregation of proteins and RNAs.
1983,
Pubmed De Robertis,
Intracellular transport of microinjected 5S and small nuclear RNAs.
1982,
Pubmed
,
Xenbase Forbes,
Small nuclear RNA transcription and ribonucleoprotein assembly in early Xenopus development.
1983,
Pubmed
,
Xenbase Galli,
Biochemical complementation with RNA in the Xenopus oocyte: a small RNA is required for the generation of 3' histone mRNA termini.
1983,
Pubmed
,
Xenbase Garber,
Genomic and cDNA clones of the homeotic locus Antennapedia in Drosophila.
1983,
Pubmed Humphries,
Recombinant plasmids containing Xenopus laevis globin structural genes derived from complementary DNA.
1978,
Pubmed
,
Xenbase Lerner,
Are snRNPs involved in splicing?
1980,
Pubmed Lerner,
Monoclonal antibodies to nucleic acid-containing cellular constituents: probes for molecular biology and autoimmune disease.
1981,
Pubmed
,
Xenbase Lerner,
Snurps and scyrps.
1981,
Pubmed Lund,
True genes for human U1 small nuclear RNA. Copy number, polymorphism, and methylation.
1984,
Pubmed Lund,
U1 small nuclear RNA genes are located on human chromosome 1 and are expressed in mouse-human hybrid cells.
1983,
Pubmed Manser,
Human U1 loci: genes for human U1 RNA have dramatically similar genomic environments.
1982,
Pubmed Marzluff,
Isolation and characterization of two linked mouse U1b small nuclear RNA genes.
1983,
Pubmed Mattaj,
Xenopus laevis U2 snRNA genes: tandemly repeated transcription units sharing 5' and 3' flanking homology with other RNA polymerase II transcribed genes.
1983,
Pubmed
,
Xenbase Matter,
Molecular characterization of ribonucleoprotein antigens bound by antinuclear antibodies. A diagnostic evaluation.
1982,
Pubmed Maxam,
Sequencing end-labeled DNA with base-specific chemical cleavages.
1980,
Pubmed Messing,
A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
1982,
Pubmed Monstein,
A candidate gene for human U1 RNA.
1982,
Pubmed Mount,
Sequence of U1 RNA from Drosophila melanogaster: implications for U1 secondary structure and possible involvement in splicing.
1981,
Pubmed Murphy,
Transcription of a gene for human U1 small nuclear RNA.
1982,
Pubmed
,
Xenbase Newport,
A major developmental transition in early Xenopus embryos: II. Control of the onset of transcription.
1982,
Pubmed
,
Xenbase Nishikura,
Genetic analysis of the processing of a spliced tRNA.
1982,
Pubmed
,
Xenbase Ohshima,
Novel models for RNA splicing that involve a small nuclear RNA.
1981,
Pubmed Padgett,
Splicing of messenger RNA precursors is inhibited by antisera to small nuclear ribonucleoprotein.
1983,
Pubmed Rogers,
A mechanism for RNA splicing.
1980,
Pubmed Roop,
Structure and expression of a chicken gene coding for U1 RNA.
1981,
Pubmed Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed Southern,
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
1975,
Pubmed Tani,
Molecular cloning and characterization of a gene for rat U2 small nuclear RNA.
1983,
Pubmed Wahli,
Isolation of two closely related vitellogenin genes, including their flanking regions, from a Xenopus laevis gene library.
1980,
Pubmed
,
Xenbase Watanabe-Nagasu,
Structural analysis of gene loci for rat U1 small nuclear RNA.
1983,
Pubmed Wise,
Dictyostelium small nuclear RNA D2 is homologous to rat nucleolar RNA U3 and is encoded by a dispersed multigene family.
1980,
Pubmed Yang,
A small nuclear ribonucleoprotein is required for splicing of adenoviral early RNA sequences.
1981,
Pubmed Zeller,
Nucleocytoplasmic distribution of snRNPs and stockpiled snRNA-binding proteins during oogenesis and early development in Xenopus laevis.
1983,
Pubmed
,
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