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???displayArticle.abstract??? TPX2 has multiple functions during mitosis, including microtubule nucleation around the chromosomes and the targeting of Xklp2 and Aurora A to the spindle. We have performed a detailed domain functional analysis of TPX2 and found that a large N-terminal domain containing the Aurora A binding peptide interacts directly with and nucleates microtubules in pure tubulin solutions. However, it cannot substitute the endogenous TPX2 to support microtubule nucleation in response to Ran guanosine triphosphate (GTP) and spindle assembly in egg extracts. By contrast, a large C-terminal domain of TPX2 that does not bind directly to pure microtubules and does not bind Aurora A kinase rescues microtubule nucleation in response to RanGTP and spindle assembly in TPX2-depleted extract. These and previous results suggest that under physiological conditions, TPX2 is essential for microtubule nucleation around chromatin and functions in a network of other molecules, some of which also are regulated by RanGTP.
Bayliss,
Structural basis of Aurora-A activation by TPX2 at the mitotic spindle.
2003, Pubmed,
Xenbase
Bayliss,
Structural basis of Aurora-A activation by TPX2 at the mitotic spindle.
2003,
Pubmed
,
Xenbase Bornens,
Studying the composition and function of centrosomes in vertebrates.
1999,
Pubmed Dasso,
The Ran GTPase: theme and variations.
2002,
Pubmed Dasso,
Running on Ran: nuclear transport and the mitotic spindle.
2001,
Pubmed Desai,
The use of Xenopus egg extracts to study mitotic spindle assembly and function in vitro.
1999,
Pubmed
,
Xenbase Elowitz,
Photoactivation turns green fluorescent protein red.
1997,
Pubmed Eyers,
A novel mechanism for activation of the protein kinase Aurora A.
2003,
Pubmed
,
Xenbase Garrett,
hTPX2 is required for normal spindle morphology and centrosome integrity during vertebrate cell division.
2002,
Pubmed
,
Xenbase Giet,
The Xenopus laevis aurora-related protein kinase pEg2 associates with and phosphorylates the kinesin-related protein XlEg5.
1999,
Pubmed
,
Xenbase Giet,
Drosophila Aurora A kinase is required to localize D-TACC to centrosomes and to regulate astral microtubules.
2002,
Pubmed
,
Xenbase Glover,
Mutations in aurora prevent centrosome separation leading to the formation of monopolar spindles.
1995,
Pubmed Görlich,
Isolation of a protein that is essential for the first step of nuclear protein import.
1994,
Pubmed
,
Xenbase Gruss,
Chromosome-induced microtubule assembly mediated by TPX2 is required for spindle formation in HeLa cells.
2002,
Pubmed
,
Xenbase Gruss,
Ran induces spindle assembly by reversing the inhibitory effect of importin alpha on TPX2 activity.
2001,
Pubmed
,
Xenbase Heald,
Spindles get the ran around.
2000,
Pubmed Heald,
Self-organization of microtubules into bipolar spindles around artificial chromosomes in Xenopus egg extracts.
1996,
Pubmed
,
Xenbase Hyman,
Preparation of modified tubulins.
1991,
Pubmed Kahana,
Cell cycle. Some importin news about spindle assembly.
2001,
Pubmed
,
Xenbase Karsenti,
The mitotic spindle: a self-made machine.
2001,
Pubmed
,
Xenbase Kufer,
Human TPX2 is required for targeting Aurora-A kinase to the spindle.
2002,
Pubmed
,
Xenbase Roghi,
The Xenopus protein kinase pEg2 associates with the centrosome in a cell cycle-dependent manner, binds to the spindle microtubules and is involved in bipolar mitotic spindle assembly.
1998,
Pubmed
,
Xenbase Sawin,
Photoactivation of green fluorescent protein.
1997,
Pubmed Sawin,
Mitotic spindle assembly by two different pathways in vitro.
1991,
Pubmed
,
Xenbase Schatz,
Importin alpha-regulated nucleation of microtubules by TPX2.
2003,
Pubmed
,
Xenbase Trieselmann,
Ran modulates spindle assembly by regulating a subset of TPX2 and Kid activities including Aurora A activation.
2003,
Pubmed
,
Xenbase Tsai,
A Ran signalling pathway mediated by the mitotic kinase Aurora A in spindle assembly.
2003,
Pubmed
,
Xenbase Weis,
Characterization of the nuclear protein import mechanism using Ran mutants with altered nucleotide binding specificities.
1996,
Pubmed Wilde,
Ran stimulates spindle assembly by altering microtubule dynamics and the balance of motor activities.
2001,
Pubmed
,
Xenbase Wittmann,
Recombinant p50/dynamitin as a tool to examine the role of dynactin in intracellular processes.
1999,
Pubmed
,
Xenbase Wittmann,
TPX2, A novel xenopus MAP involved in spindle pole organization.
2000,
Pubmed
,
Xenbase Wittmann,
Localization of the kinesin-like protein Xklp2 to spindle poles requires a leucine zipper, a microtubule-associated protein, and dynein.
1998,
Pubmed
,
Xenbase
