Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Structure and transmembrane nature of the acetylcholine receptor in amphibian skeletal muscle as revealed by cross-reacting monoclonal antibodies.
Sargent PB, Hedges BE, Tsavaler L, Clemmons L, Tzartos S, Lindstrom JM.
???displayArticle.abstract???
A collection of 126 monoclonal antibodies (mAbs) made against acetylcholine receptors (AChRs) from the electric organs of Torpedo californica or Electrophorus electricus was tested for cross-reactivity with AChRs in cryostat sections of skeletal muscle from Rana pipiens and Xenopus laevis by indirect immunofluorescence. 49 mAbs (39%) cross-reacted with AChRs from Rana, and 25 mAbs (20%) cross-reacted with AChRs from Xenopus. mAbs specific for each of the four subunits of electric organ AChR (alpha, beta, gamma, delta) cross-reacted with AChRs from each amphibian species. mAbs cross-reacting with Xenopus AChRs were, with one exception, a subset of the mAbs cross-reacting with Rana AChRs. The major difference detected between the two species was in binding by mAbs specific for the main immunogenic region (MIR) of the alpha-subunit. Whereas 22 of 33 anti-MIR mAbs tested cross-reacted with Rana AChRs, only one of these mAbs cross-reacted with Xenopus AChRs. Some (32) of the cross-reacting mAbs were tested for binding to AChRs in intact muscle. 21 of these mAbs bound to AChRs only when membranes were made permeable with saponin. Electron microscopy using immunoperoxidase or colloidal gold techniques revealed that these mAbs recognize cytoplasmic determinants and that mAbs that do not require saponin in order to bind AChRs in intact muscle recognize extracellular determinants. These results suggest that AChRs in skeletal muscle of Rana and Xenopus are composed of subunits corresponding to the alpha-, beta-, gamma-, and delta-subunits of AChRs from fish electric organs. The subunit specificity of mAbs whose binding was examined by electron microscopy suggests that parts of each subunit (alpha, beta, gamma, delta) are exposed on the cytoplasmic surface and that, as in AChRs from fish electric organs and mammalian muscle, the MIR on alpha-subunits of Rana AChRs is exposed on the extracellular surface.
Anderson,
In vitro synthesis, glycosylation, and membrane insertion of the four subunits of Torpedo acetylcholine receptor.
1981, Pubmed
Anderson,
In vitro synthesis, glycosylation, and membrane insertion of the four subunits of Torpedo acetylcholine receptor.
1981,
Pubmed Anderson,
Fluorescent staining of acetylcholine receptors in vertebrate skeletal muscle.
1974,
Pubmed Anderson,
Transmembrane orientation of an early biosynthetic form of acetylcholine receptor delta subunit determined by proteolytic dissection in conjunction with monoclonal antibodies.
1983,
Pubmed Anderson,
Signal recognition protein is required for the integration of acetylcholine receptor delta subunit, a transmembrane glycoprotein, into the endoplasmic reticulum membrane.
1982,
Pubmed Burden,
Acetylcholine receptors at neuromuscular synapses: phylogenetic differences detected by snake alpha-neurotoxins.
1975,
Pubmed Claudio,
Nucleotide and deduced amino acid sequences of Torpedo californica acetylcholine receptor gamma subunit.
1983,
Pubmed Conti-Tronconi,
Subunit structure of the acetylcholine receptor from Electrophorus electricus.
1982,
Pubmed Conti-Tronconi,
Monoclonal antibodies as probes of acetylcholine receptor structure. 2. Binding to native receptor.
1981,
Pubmed Conti-Tronconi,
Mammalian muscle acetylcholine receptor: a supramolecular structure formed by four related proteins.
1982,
Pubmed Devillers-Thiery,
Complete mRNA coding sequence of the acetylcholine binding alpha-subunit of Torpedo marmorata acetylcholine receptor: a model for the transmembrane organization of the polypeptide chain.
1983,
Pubmed Einarson,
Subunit composition of bovine muscle acetylcholine receptor.
1982,
Pubmed Froehner,
Identification of exposed and buried determinants of the membrane-bound acetylcholine receptor from Torpedo californica.
1981,
Pubmed Froehner,
Monoclonal antibodies to cytoplasmic domains of the acetylcholine receptor.
1983,
Pubmed Gullick,
Structural similarities between acetylcholine receptors from fish electric organs and mammalian muscle.
1982,
Pubmed Gullick,
Monoclonal antibodies as probes of acetylcholine receptor structure. 1. Peptide mapping.
1981,
Pubmed Gullick,
Mapping the binding of monoclonal antibodies to the acetylcholine receptor from Torpedo californica.
1983,
Pubmed Hartzell,
Post-synaptic potentiation: interaction between quanta of acetylcholine at the skeletal neuromuscular synapse.
1975,
Pubmed Hsu,
Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures.
1981,
Pubmed Ishikawa,
Fluorescent staining of neuromuscular junctions by using the antibody against acetylcholine receptors of Narke japonica, and double staining with the antibody and erabutoxin b.
1981,
Pubmed Jones,
Absence of [125I] alpha-bungarotoxin binding to motor nerve terminals of frog, lizard and mouse muscle.
1983,
Pubmed KARNOVSKY,
THE LOCALIZATION OF CHOLINESTERASE ACTIVITY IN RAT CARDIAC MUSCLE BY ELECTRON MICROSCOPY.
1964,
Pubmed Kuffler,
The distribution of acetylcholine sensitivity at the post-synaptic membrane of vertebrate skeletal twitch muscles: iontophoretic mapping in the micron range.
1975,
Pubmed Lentz,
Synaptic vesicle recycling at the neuromuscular junction in the presence of a presynaptic membrane marker.
1982,
Pubmed Lindstrom,
Purification of acetylcholine receptors from the muscle of Electrophorus electricus.
1983,
Pubmed Lindstrom,
Biochemical properties of acteylcholine receptor subunits from Torpedo californica.
1979,
Pubmed Lindstrom,
Immunochemical similarities between subunits of acetylcholine receptors from Torpedo, Electrophorus, and mammalian muscle.
1979,
Pubmed Lindstrom,
Immunization of rats with polypeptide chains from torpedo acetylcholine receptor causes an autoimmune response to receptors in rat muscle.
1978,
Pubmed Lindstrom,
Acetylcholine receptors from Torpedo and Electrophorus have similar subunit structures.
1980,
Pubmed Matthews-Bellinger,
Distribution of acetylcholine receptors at frog neuromuscular junctions with a discussion of some physiological implications.
1978,
Pubmed Mellinger,
Electric organ development in Torpedo marmorata, Chondrichthyes.
1978,
Pubmed Noda,
Primary structures of beta- and delta-subunit precursors of Torpedo californica acetylcholine receptor deduced from cDNA sequences.
1983,
Pubmed Noda,
Structural homology of Torpedo californica acetylcholine receptor subunits.
1983,
Pubmed Noda,
Primary structure of alpha-subunit precursor of Torpedo californica acetylcholine receptor deduced from cDNA sequence.
1982,
Pubmed Peper,
The acetylcholine receptor at the neuromuscular junction.
1982,
Pubmed Raftery,
Acetylcholine receptor: complex of homologous subunits.
1980,
Pubmed Reynolds,
Molecular weight in detergent solution of acetylcholine receptor from Torpedo californica.
1978,
Pubmed Ross,
Structural studies of a membrane-bound acetylcholine receptor from Torpedo californica.
1977,
Pubmed St John,
Nicotinic postsynaptic membranes from Torpedo: sidedness, permeability to macromolecules, and topography of major polypeptides.
1982,
Pubmed Strader,
Topographic studies of Torpedo acetylcholine receptor subunits as a transmembrane complex.
1980,
Pubmed Swanson,
Immunohistochemical localization of monoclonal antibodies to the nicotinic acetylcholine receptor in chick midbrain.
1983,
Pubmed Tzartos,
Monoclonal antibodies used to probe acetylcholine receptor structure: localization of the main immunogenic region and detection of similarities between subunits.
1980,
Pubmed Tzartos,
Mapping of surface structures of electrophorus acetylcholine receptor using monoclonal antibodies.
1981,
Pubmed Tzartos,
Specificities of antibodies to acetylcholine receptors in sera from myasthenia gravis patients measured by monoclonal antibodies.
1982,
Pubmed Vandlen,
Studies of the composition of purified Torpedo californica acetylcholine receptor and of its subunits.
1979,
Pubmed
