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XB-ART-30069
Cell Differ 1983 Oct 01;132:143-8.
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Stabilization of the maturation promoting factor (MPF) from Xenopus laevis oocytes. Protection against calcium ions.

Hermann J, Bellé R, Tso J, Ozon R.


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Maturation promoting factor (MPF) activity was recovered from progesterone-matured Xenopus oocytes cytosol, fractionated by polyethylene glycol-20,000 or ethanolic precipitation. An improved stabilization of the biological activity was obtained by adding adenosine 5'-O-(3-thiotriphosphate) (ATP-gamma-S) (50 microM) to the preparation buffers. Neither Ca2+ ions nor calmodulin inhibit the partially purified MPF.

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Species referenced: Xenopus laevis
Genes referenced: cdk1