Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
The decapeptide caerulein represents one of the main constituents of the skin secretion of Xenopus laevis. Total mRNA was isolated from skin, transcribed into cDNA and inserted via GC-tailing into the plasmid pUC8. Among the transformants, 300 clones were selected at random and screened with a cDNA primed with the synthetic deoxynucleotide d(AGTCCATCCA), which is complementary to the mRNA region coding for the fragment Trp-Met-Asp-Phe of cerulein. Of nine strongly hybridizing clones, three were sequenced and these were found to contain inserts with very similar nucleotide sequences. The cloned cDNAs code for parts of two different caerulein precursors. These contain one or two copies of caerulein and five additional amino acids located between pairs of arginine residues. The extra glycine at the carboxy terminus is considered to serve as the signal for amidation, while the tetrapeptide Phe-Ala-Asp-Gly, linked to the amino end of caerulein in these precursors, must be cleaved by an unusual processing mechanism.
Ackermann,
Demonstration of G . U wobble base pairs by Raman and IR spectroscopy.
1979, Pubmed
Ackermann,
Demonstration of G . U wobble base pairs by Raman and IR spectroscopy.
1979,
Pubmed Amara,
Characterization of rat calcitonin mRNA.
1980,
Pubmed Anastasi,
Presence of caerulein in extracts of the skin of Leptodactylus pentadactylus labyrinthicus and of Xenopus laevis.
1970,
Pubmed
,
Xenbase Anastasi,
Isolation and amino acid sequence of caerulein, the active decapeptide of the skin of hyla caerulea.
1968,
Pubmed Aviv,
Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
1972,
Pubmed Birnboim,
A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
1979,
Pubmed Bradbury,
Mechanism of C-terminal amide formation by pituitary enzymes.
1982,
Pubmed Comb,
Primary structure of the human Met- and Leu-enkephalin precursor and its mRNA.
1982,
Pubmed Erspamer,
Biogenic amines and active polypeptides of the amphibian skin.
1971,
Pubmed Fritz,
High-pressure liquid chromatography in polynucleotide synthesis.
1978,
Pubmed Glisin,
Ribonucleic acid isolated by cesium chloride centrifugation.
1974,
Pubmed Grunstein,
Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
1975,
Pubmed Jay,
DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping.
1974,
Pubmed Kakidani,
Cloning and sequence analysis of cDNA for porcine beta-neo-endorphin/dynorphin precursor.
1982,
Pubmed Kreil,
Stepwise cleavage of the pro part of promelittin by dipeptidylpeptidase IV. Evidence for a new type of precursor--product conversion.
1980,
Pubmed Kurjan,
Structure of a yeast pheromone gene (MF alpha): a putative alpha-factor precursor contains four tandem copies of mature alpha-factor.
1982,
Pubmed Land,
Nucleotide sequence of cloned cDNA encoding bovine arginine vasopressin-neurophysin II precursor.
1982,
Pubmed Larsson,
Evidence for a common evolutionary origin of gastrin and cholecystokinin.
1977,
Pubmed Lehrach,
Construction and characterization of pro alpha 1 collagen complementary deoxyribonucleic acid clones.
1979,
Pubmed Mandel,
Calcium-dependent bacteriophage DNA infection.
1970,
Pubmed Maxam,
Sequencing end-labeled DNA with base-specific chemical cleavages.
1980,
Pubmed McDonell,
Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.
1977,
Pubmed Nakanishi,
Nucleotide sequence of cloned cDNA for bovine corticotropin-beta-lipotropin precursor.
1979,
Pubmed Narang,
Improved phosphotriester method for the synthesis of gene fragments.
1979,
Pubmed Narang,
Chemical synthesis of deoxyoligonucleotides by the modified triester method.
1980,
Pubmed Noda,
Cloning and sequence analysis of cDNA for bovine adrenal preproenkephalin.
1982,
Pubmed Noyes,
Detection and partial sequence analysis of gastrin mRNA by using an oligodeoxynucleotide probe.
1979,
Pubmed Proudfoot,
3' non-coding region sequences in eukaryotic messenger RNA.
1976,
Pubmed Rehfeld,
Neural regulation of pancreatic hormone secretion by the C-terminal tetrapeptide of CCK.
1980,
Pubmed Ricciardi,
Purification and mapping of specific mRNAs by hybridization-selection and cell-free translation.
1979,
Pubmed Rowekamp,
Isolation of developmentally regulated genes from Dictyostelium.
1980,
Pubmed Steiner,
Proteolytic processing in the biosynthesis of insulin and other proteins.
1974,
Pubmed Suchanek,
Translation of melittin messenger RNA in vitro yields a product terminating with glutaminylglycine rather than with glutaminamide.
1977,
Pubmed Yoo,
Molecular cloning and nucleotide sequence of full-length of cDNA coding for porcine gastrin.
1982,
Pubmed Young,
The role of the Golgi complex in sulfate metabolism.
1973,
Pubmed
