Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
An in vitro RNA polymerase III system from S. cerevisiae: effects of deletions and point mutations upon SUP4 gene transcription.
Koski RA, Allison DS, Worthington M, Hall BD.
???displayArticle.abstract???
A soluble cell-free extract containing RNA polymerase III and factors essential for selective transcription of the yeast SUP4-o tRNATyr gene was prepared from Saccharomyces cerevisiae cells. An intragenic promoter for yeast RNA polymerase III was identified within the yeast tRNATyr coding sequence by testing several sup4 genes with 5'- and 3'-terminal deletions in the homologous transcription system. Thirty-four different sup4 genes with spontaneous mutations were also tested in the in vitro system. Two point mutations drastically reduced transcription initiation and two other mutations caused premature termination. These mutations have nearly identical effects on SUP4 gene transcription by Xenopus RNA polymerase III (1), which demonstrates that the essential features of RNA polymerase III transcription initiation and termination signals have been conserved throughout the course of eukaryotic evolution.
Adman,
Transcription in yeast: separation and properties of multiple FNA polymerases.
1972, Pubmed
Adman,
Transcription in yeast: separation and properties of multiple FNA polymerases.
1972,
Pubmed Bogenhagen,
A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3' border of the region.
1980,
Pubmed
,
Xenbase Bogenhagen,
Nucleotide sequences in Xenopus 5S DNA required for transcription termination.
1981,
Pubmed
,
Xenbase Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed Celis,
Collection of mutant tRNA sequences.
1980,
Pubmed Ciliberto,
Promoter of a eukaryotic tRNAPro gene is composed of three noncontiguous regions.
1982,
Pubmed
,
Xenbase Clarke,
Isolation of a yeast centromere and construction of functional small circular chromosomes.
1980,
Pubmed DeFranco,
Two control regions for eukaryotic tRNA gene transcription.
1980,
Pubmed
,
Xenbase De Robertis,
Transcription and processing of cloned yeast tyrosine tRNA genes microinjected into frog oocytes.
1979,
Pubmed
,
Xenbase Engelke,
Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes.
1980,
Pubmed
,
Xenbase Faye,
Deletion mapping of sequences essential for in vivo transcription of the iso-1-cytochrome c gene.
1981,
Pubmed Fowlkes,
Transcriptional control regions of the adenovirus VAI RNA gene.
1980,
Pubmed Galli,
Two conserved sequence blocks within eukaryotic tRNA genes are major promoter elements.
1981,
Pubmed
,
Xenbase Goodman,
Nucleotide sequence of a mutant eukaryotic gene: the yeast tyrosine-inserting ochre suppressor SUP4-o.
1977,
Pubmed Guilfoyle,
Control region for adenovirus VA RNA transcription.
1981,
Pubmed Hall,
Transcription initiation of eucaryotic transfer RNA genes.
1982,
Pubmed Hawthorne,
Suppressors in yeast.
1974,
Pubmed Hofstetter,
A split promoter for a eucaryotic tRNA gene.
1981,
Pubmed
,
Xenbase Hopper,
tRNA synthesis: identification of in vivo precursor tRNAs from parental and mutant yeast strains.
1981,
Pubmed
,
Xenbase Klekamp,
Specific transcription of homologous class III genes in yeast-soluble cell-free extracts.
1982,
Pubmed Koski,
Mutations of the yeast SUP4 tRNATyr locus: transcription of the mutant genes in vitro.
1980,
Pubmed
,
Xenbase Kressmann,
A tRNA gene of Xenopus laevis contains at least two sites promoting transcription.
1979,
Pubmed
,
Xenbase Kurjan,
Mutations at the yeast SUP4 tRNATyr locus: DNA sequence changes in mutants lacking suppressor activity.
1980,
Pubmed Manley,
DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract.
1980,
Pubmed Price,
The Developmental Genetics of the CALCAROIDES Gene in Barley, II. Peroxidase Activity in Mutant and Normal Plants at Progressive Stages of Development.
1971,
Pubmed Rosa,
Striking similarities are exhibited by two small Epstein-Barr virus-encoded ribonucleic acids and the adenovirus-associated ribonucleic acids VAI and VAII.
1981,
Pubmed Rothstein,
A genetic fine structure analysis of the suppressor 3 locus in Saccharomyces.
1977,
Pubmed Sakonju,
A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5' border of the region.
1980,
Pubmed
,
Xenbase Schultz,
Transcription in yeast: alpha-amanitin sensitivity and other properties which distinguish between RNA polymerases I and III.
1976,
Pubmed Segall,
Multiple factors are required for the accurate transcription of purified genes by RNA polymerase III.
1980,
Pubmed Sharp,
Internal control regions for transcription of eukaryotic tRNA genes.
1981,
Pubmed
,
Xenbase Smith,
Sequence of the gene for iso-1-cytochrome c in Saccharomyces cerevisiae.
1979,
Pubmed Sprague,
5' flanking sequence signals are required for activity of silkworm alanine tRNA genes in homologous in vitro transcription systems.
1980,
Pubmed
,
Xenbase Sprinzl,
Compilation of tRNA sequences.
1980,
Pubmed Struhl,
High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.
1979,
Pubmed Sugden,
Mammalian deoxyribonucleic acid-dependent ribonucleic acid polymerases. I. Purification and properties of an -amanitin-sensitive ribonucleic acid polymerase and stimulatory factors from HeLa and KB cells.
1973,
Pubmed
