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Simple, efficient in vitro synthesis of capped RNA useful for direct expression of cloned eukaryotic genes.
Contreras R, Cheroutre H, Degrave W, Fiers W.
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A simple and efficient method for direct in vitro synthesis of capped transcripts of cloned eukaryotic genes is described. As an example capped transcripts were made from a plasmid containing the human fibroblast interferon gene cloned under the control of a prokaryotic promoter. These transcripts were translated in vivo in Xenopus laevis oocytes and in vitro in reticulocyte and in wheat germ cell-free protein synthesizing systems.
Contreras,
Nucleotide sequence of the restriction fragment Hind F-Eco RI2 of SV40 DNA.
1977, Pubmed
Contreras,
Nucleotide sequence of the restriction fragment Hind F-Eco RI2 of SV40 DNA.
1977,
Pubmed Contreras,
Proceedings: Controlled transcription of the synthetic gene for tyrosine transfer RNA.
1976,
Pubmed Contreras,
A simple apparatus for injection of nanoliter quantities into Xenopus laevis oocytes.
1981,
Pubmed
,
Xenbase Derynck,
Expression of human fibroblast interferon gene in Escherichia coli.
1980,
Pubmed Derynck,
Isolation and structure of a human fibroblast interferon gene.
1980,
Pubmed Downey,
Studies on the kinetics of ribonucleic acid chain initiation and elongation.
1970,
Pubmed Downey,
Determination of nucleotide sequences at promoter regions by the use of dinucleotides.
1971,
Pubmed Fiers,
Complete nucleotide sequence of SV40 DNA.
1978,
Pubmed Gheysen,
Systematic alteration of the nucleotide sequence preceding the translation initiation codon and the effects on bacterial expression of the cloned SV40 small-t antigen gene.
1982,
Pubmed Kleppe,
Studies on polynucleotides. CXIX. Transcription of short double-stranded deoxyribonucleic acids of defined nucleotide sequences.
1972,
Pubmed Kozak,
How do eucaryotic ribosomes select initiation regions in messenger RNA?
1978,
Pubmed Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed Paterson,
Efficient translation of prokaryotic mRNAs in a eukaryotic cell-free system requires addition of a cap structure.
1979,
Pubmed Remaut,
Plasmid vectors for high-efficiency expression controlled by the PL promoter of coliphage lambda.
1981,
Pubmed Roberts,
Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ.
1973,
Pubmed Shine,
The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.
1974,
Pubmed Smith,
Analysis of RNA initiated in isolated mouse myeloma nuclei using purine nucleoside 5'[gamma-S]triphosphates as affinity probes.
1978,
Pubmed Terao,
Studies on polynucleotides. CXX. On the transcription of a synthetic 29-unit long deoxyribopolynucleotide.
1972,
Pubmed Volckaert,
Analysis of 32P-labeled bacteriophage MS2 RNA by a mini-fingerprinting procedure.
1976,
Pubmed Wei,
Methylation of newly synthesized viral messenger RNA by an enzyme in vaccinia virus.
1974,
Pubmed Wimmer,
Sequence studies of satellite tobacco necrosis virus RNA. Isolation and characterization of a 5'-terminal trinucleotide.
1968,
Pubmed
