Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Expression of a X. laevis tRNATyr gene in mammalian cells.
Laski FA, Alzner-DeWeerd B, RajBhandary UL, Sharp PA.
???displayArticle.abstract???
Expression of a X. laevis tRNATyr gene has been studied in mammalian cells. This tRNATyr gene has a 13 base intervening sequence adjacent to its anticodon. A fragment containing the tRNATyr gene was cloned into the late region of SV40. Cells infected with a recombinant virus stock vastly overproduce a tRNATyr that is properly spliced, processed and modified. It was also found that the X. laevis tRNATyr is identical or nearly identical to an endogenous tRNATyr of monkey kidney cells. The possibility of using the X. laevis tRNATyr gene to create an amber suppressor for mammalian cells is discussed.
???displayArticle.pubmedLink???
6290987 ???displayArticle.pmcLink???PMC321116 ???displayArticle.link???Nucleic Acids Res ???displayArticle.grants???[+]
Bolivar,
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
1977, Pubmed
Bolivar,
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
1977,
Pubmed Brambilla,
Unexpected occurrence of an aminoacylated nucleoside in mammalian tRNATyr.
1976,
Pubmed Chu,
SV40 DNA transfection of cells in suspension: analysis of efficiency of transcription and translation of T-antigen.
1981,
Pubmed Clarkson,
Sequence organization of a cloned tDNA met fragment from Xenopus laevis.
1978,
Pubmed
,
Xenbase Cremer,
In vitro suppression of UAG and UGA mutants in the thymidine kinase gene of herpes simplex virus.
1979,
Pubmed Dagert,
Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
1979,
Pubmed Gilmore,
Super-suppressors in Saccharomyces cerevisiae.
1967,
Pubmed Goff,
Construction, propagation and expression of simian virus 40 recombinant genomes containing the Escherichia coli gene for thymidine kinase and a Saccharomyces cerevisae gene for tyrosine transfer RNA.
1979,
Pubmed Graham,
A new technique for the assay of infectivity of human adenovirus 5 DNA.
1973,
Pubmed HAWTHORNE,
Super-suppressors in yeast.
1963,
Pubmed Hirt,
Selective extraction of polyoma DNA from infected mouse cell cultures.
1967,
Pubmed Hutchison,
Mutagenesis at a specific position in a DNA sequence.
1978,
Pubmed Knapp,
Splicing of yeast tRNA precursors: structure of the reaction intermediates.
1979,
Pubmed Kudo,
Site-specific mutagenesis on cloned DNAs: generation of a mutant of Escherichia coli tyrosine suppressor tRNA in which the sequence G-T-T-C corresponding to the universal G-T-pseudouracil-C sequence of tRNAs is changed to G-A-T-C.
1981,
Pubmed Müller,
Nucleotide sequence of genes coding for tRNAPhe and tRNATyr from a repeating unit of X. laevis DNA.
1980,
Pubmed
,
Xenbase Mulligan,
Synthesis of rabbit beta-globin in cultured monkey kidney cells following infection with a SV40 beta-globin recombinant genome.
1979,
Pubmed Philipson,
Translation of MuLV and MSV RNAs in nuclease-treated reticulocyte extracts: enhancement of the gag-pol polypeptide with yeast suppressor tRNA.
1978,
Pubmed Rawlins,
Construction of a specific amber codon in the simian virus 40 T-antigen gene by site-directed mutagenesis.
1980,
Pubmed Razin,
Efficient correction of a mutation by use of chemically synthesized DNA.
1978,
Pubmed Silberklang,
Use of in vitro 32P labeling in the sequence analysis of nonradioactive tRNAs.
1979,
Pubmed Temple,
Construction of a functional human suppressor tRNA gene: an approach to gene therapy for beta-thalassaemia.
1982,
Pubmed Weiner,
An abundant cytoplasmic 7S RNA is complementary to the dominant interspersed middle repetitive DNA sequence family in the human genome.
1980,
Pubmed
