Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
The general transcription factor TATA-binding protein (TBP) is a key initiation factor involved in transcription by all three eukaryotic RNA polymerases. In addition, the related metazoan-specific TBP-like factor (TLF/TRF2) is essential for transcription of a distinct subset of genes. Here we characterize the vertebrate-specific TBP-like factorTBP2, using in vitro assays, in vivo antisense knockdown, and mRNA rescue experiments, as well as chromatin immunoprecipitation. We show that TBP2 is recruited to promoters in Xenopus oocytes in the absence of detectable TBP recruitment. Furthermore, TBP2 is essential for gastrulation and for the transcription of a subset of genes during Xenopus embryogenesis. In embryos, TBP2 protein is much less abundant than TBP, and moderate overexpression of TBP2 partially rescues an antisense knockdown of TBP levels and restores transcription of many TBP-dependent genes. TBP2 may be a TBP replacement factor in oocytes, whereas in embryos both TBP and TBP2 are required even though they exhibit partial redundancy and gene selectivity.
Bártfai,
TBP2, a vertebrate-specific member of the TBP family, is required in embryonic development of zebrafish.
2004, Pubmed
Bártfai,
TBP2, a vertebrate-specific member of the TBP family, is required in embryonic development of zebrafish.
2004,
Pubmed Brand,
UV-damaged DNA-binding protein in the TFTC complex links DNA damage recognition to nucleosome acetylation.
2001,
Pubmed Dagle,
Oligonucleotide-based strategies to reduce gene expression.
2001,
Pubmed
,
Xenbase Dagle,
Targeted elimination of zygotic messages in Xenopus laevis embryos by modified oligonucleotides possessing terminal cationic linkages.
2000,
Pubmed
,
Xenbase Dantonel,
TBP-like factor is required for embryonic RNA polymerase II transcription in C. elegans.
2000,
Pubmed Dantonel,
The TBP-like factor: an alternative transcription factor in metazoa?
1999,
Pubmed Davidson,
The genetics of TBP and TBP-related factors.
2003,
Pubmed Hansen,
Transcription properties of a cell type-specific TATA-binding protein, TRF.
1997,
Pubmed Hernandez,
TBP, a universal eukaryotic transcription factor?
1993,
Pubmed Hobbs,
Removing the vertebrate-specific TBP N terminus disrupts placental beta2m-dependent interactions with the maternal immune system.
2002,
Pubmed Hochheimer,
Diversified transcription initiation complexes expand promoter selectivity and tissue-specific gene expression.
2003,
Pubmed Holmes,
Promoter-selective properties of the TBP-related factor TRF1.
2000,
Pubmed Kaltenbach,
The TBP-like factor CeTLF is required to activate RNA polymerase II transcription during C. elegans embryogenesis.
2000,
Pubmed Kass,
DNA methylation directs a time-dependent repression of transcription initiation.
1997,
Pubmed
,
Xenbase Li,
Involvement of histone methylation and phosphorylation in regulation of transcription by thyroid hormone receptor.
2002,
Pubmed
,
Xenbase Ma,
Isolation of a cDNA encoding the largest subunit of TFIIA reveals functions important for activated transcription.
1993,
Pubmed Maldonado,
Factors involved in specific transcription by mammalian RNA polymerase II: role of transcription factors IIA, IID, and IIB during formation of a transcription-competent complex.
1990,
Pubmed Martianov,
Late arrest of spermiogenesis and germ cell apoptosis in mice lacking the TBP-like TLF/TRF2 gene.
2001,
Pubmed Martianov,
RNA polymerase II transcription in murine cells lacking the TATA binding protein.
2002,
Pubmed Mitsiou,
TAC, a TBP-sans-TAFs complex containing the unprocessed TFIIAalphabeta precursor and the TFIIAgamma subunit.
2000,
Pubmed Mittal,
Role for the amino-terminal region of human TBP in U6 snRNA transcription.
1997,
Pubmed Moore,
A human TATA binding protein-related protein with altered DNA binding specificity inhibits transcription from multiple promoters and activators.
1999,
Pubmed Müller,
TBP is not universally required for zygotic RNA polymerase II transcription in zebrafish.
2001,
Pubmed Paulson,
IFN-Stimulated transcription through a TBP-free acetyltransferase complex escapes viral shutoff.
2002,
Pubmed Peng,
Xenopus laevis: Practical uses in cell and molecular biology. Solutions and protocols.
1991,
Pubmed
,
Xenbase Persengiev,
TRF3, a TATA-box-binding protein-related factor, is vertebrate-specific and widely expressed.
2003,
Pubmed Rabenstein,
TATA box-binding protein (TBP)-related factor 2 (TRF2), a third member of the TBP family.
1999,
Pubmed Rietveld,
In vivo repression of an erythroid-specific gene by distinct corepressor complexes.
2002,
Pubmed Roeder,
The role of general initiation factors in transcription by RNA polymerase II.
1996,
Pubmed Ruppert,
Monoclonal antibodies directed against the amino-terminal domain of human TBP cross-react with TBP from other species.
1996,
Pubmed Schmidt,
Fundamental cellular processes do not require vertebrate-specific sequences within the TATA-binding protein.
2003,
Pubmed Takada,
A TRF1:BRF complex directs Drosophila RNA polymerase III transcription.
2000,
Pubmed Teichmann,
Human TATA-binding protein-related factor-2 (hTRF2) stably associates with hTFIIA in HeLa cells.
1999,
Pubmed Veenstra,
Translation of maternal TATA-binding protein mRNA potentiates basal but not activated transcription in Xenopus embryos at the midblastula transition.
1999,
Pubmed
,
Xenbase Veenstra,
Distinct roles for TBP and TBP-like factor in early embryonic gene transcription in Xenopus.
2000,
Pubmed
,
Xenbase Veenstra,
Gene-selective developmental roles of general transcription factors.
2001,
Pubmed
,
Xenbase Wieczorek,
Function of TAF(II)-containing complex without TBP in transcription by RNA polymerase II.
1998,
Pubmed Zhang,
Spermiogenesis deficiency in mice lacking the Trf2 gene.
2001,
Pubmed
,
Xenbase Zhao,
A regulated two-step mechanism of TBP binding to DNA: a solvent-exposed surface of TBP inhibits TATA box recognition.
2002,
Pubmed Zorn,
The KH domain protein encoded by quaking functions as a dimer and is essential for notochord development in Xenopus embryos.
1997,
Pubmed
,
Xenbase
