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Molecular cloning of human immune interferon cDNA and its expression in eukaryotic cells.
Devos R, Cheroutre H, Taya Y, Degrave W, Van Heuverswyn H, Fiers W.
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Starting with mRNA derived from Staphylococcal enterotoxin A induced human splenocytes, dsDNA was synthesized and inserted into unique BamHI site of the eukaryotic expression vector pSV529 (1). A recombinant plasmid containing human immune interferon (IFN-gamma) cDNA was identified by hybridization of plasmid inserted DNA bound onto nitrocellulose filters with mRNA derived from SEA-induced splenocytes, translation of the eluted RNA in Xenopus laevis oocytes and assaying for IFN activity. Plasmids containing the entire human IFN-gamma cDNA sequence were identified by colony hybridization and were sequenced. A unique coding region was identified which predicted a protein of 166 amino acids, the 20 N-terminal amino acids of which presumably represent a signal peptide. After transfection of monkey cells with plasmid DNA isolated from one of the recombinant clones (pHIIF-SV-gamma 1), IFN was excreted into the culture medium. This IFN was not distinguishable from human IFN-gamma by serological criteria or by cell target species specificity.
Ankel,
Mouse fibroblast (type I) and immune (type II) interferons: pronounced differences in affinity for gangliosides and in antiviral and antigrowth effects on mouse leukemia L-1210R cells.
1980, Pubmed
Ankel,
Mouse fibroblast (type I) and immune (type II) interferons: pronounced differences in affinity for gangliosides and in antiviral and antigrowth effects on mouse leukemia L-1210R cells.
1980,
Pubmed Blalock,
Purified human immune interferon has more potent anticellular activity than fibroblast or leukocyte interferon.
1980,
Pubmed Branca,
Evidence that types I and II interferons have different receptors.
1981,
Pubmed Catalona,
gamma-Interferon induced by S. aureus protein A augments natural killing and ADCC.
1981,
Pubmed Claeys,
Activation of natural cytotoxicity of human peripheral blood mononuclear cells by interferon: a kinetic study and comparison of different interferon types.
1982,
Pubmed Crane,
Inhibition of murine osteogenic sarcomas by treatment with type I or type II interferon.
1978,
Pubmed Davis,
The mechanism of protein secretion across membranes.
1980,
Pubmed de Ley,
Interferon induced in human leukocytes by mitogens: production, partial purification and characterization.
1980,
Pubmed Derynck,
Isolation and structure of a human fibroblast interferon gene.
1980,
Pubmed Devos,
Construction and characterization of a plasmid containing a nearly full-size DNA copy of bacteriophage MS2 RNA.
1979,
Pubmed Dianzani,
Immune and virus-induced interferons may activate cells by different derepressional mechanisms.
1980,
Pubmed Dianzani,
Enzymatic induction of interferon production by galactose oxidase treatment of human lymphoid cells.
1979,
Pubmed Dianzani,
Human immune interferon: induction in lymphoid cells by a calcium ionophore.
1980,
Pubmed Epstein,
Evaluation of T lymphocyte effector function in immunodeficiency diseases: abnormality in mitogen-stimulated interferon in patients with selective IgA deficiency.
1974,
Pubmed Epstein,
Interferon-gamma: success, structure and speculation.
1982,
Pubmed Farrar,
Regulation of the production of immune interferon and cytotoxic T lymphocytes by interleukin 2.
1981,
Pubmed Fleischmann,
Potentiation of interferon activity by mixed preparations of fibroblast and immune interferon.
1979,
Pubmed Fleischmann,
Potentiation of antitumor effect of virus-induced interferon by mouse immune interferon preparations.
1980,
Pubmed Gray,
Expression of human immune interferon cDNA in E. coli and monkey cells.
1982,
Pubmed Green,
Immune specific induction of interferon production in cultures of human blood lymphocytes.
1969,
Pubmed Gresser,
Interferon and cell division. IX. Interferon-resistant L1210 cells: characteristics and origin.
1974,
Pubmed Grunstein,
Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
1975,
Pubmed Hanahan,
Plasmid screening at high colony density.
1980,
Pubmed Hooks,
Immune interferon in the circulation of patients with autoimmune disease.
1979,
Pubmed Kahn,
Plasmid cloning vehicles derived from plasmids ColE1, F, R6K, and RK2.
1979,
Pubmed Kozak,
How do eucaryotic ribosomes select initiation regions in messenger RNA?
1978,
Pubmed Langford,
Large-scale production and physicochemical characterization of human immune interferon.
1979,
Pubmed Mantei,
The nucleotide sequence of a cloned human leukocyte interferon cDNA.
1980,
Pubmed Maxam,
A new method for sequencing DNA.
1977,
Pubmed McCutchan,
Enchancement of the infectivity of simian virus 40 deoxyribonucleic acid with diethylaminoethyl-dextran.
1968,
Pubmed McReynolds,
Sequence of chicken ovalbumin mRNA.
1978,
Pubmed Mizrahi,
Glycosylation of interferons. Effects of tunicamycin on human immune interferon.
1978,
Pubmed Nathan,
Immune (gamma) interferon produced by a human T-lymphoblast cell line.
1981,
Pubmed Parnes,
Mouse beta 2-microglobulin cDNA clones: a screening procedure for cDNA clones corresponding to rare mRNAs.
1981,
Pubmed Perussia,
Interferon production by human and murine lymphocytes in response to alloantigens.
1980,
Pubmed Proudfoot,
3' non-coding region sequences in eukaryotic messenger RNA.
1976,
Pubmed Sonnenfeld,
Time and dosage dependence of immunoenhancement by murine type II interferon preparations.
1978,
Pubmed Taniguchi,
Partial characterization of gamma (immune) interferon mRNA extracted from human lymphocytes.
1981,
Pubmed
,
Xenbase Volckaert,
Molecular mechanisms of nucleotide-sequence rearrangements in cDNA clones of human fibroblast interferon mRNA.
1981,
Pubmed von Wussow,
Human gamma interferon production by leukocytes induced with monoclonal antibodies recognizing T cells.
1981,
Pubmed Wallace,
Translation of human immune interferon messenger RNA in Xenopus laevis oocytes.
1981,
Pubmed
,
Xenbase Wheelock,
Interferon-Like Virus-Inhibitor Induced in Human Leukocytes by Phytohemagglutinin.
1965,
Pubmed Yip,
Partial purification and characterization of human gamma (immune) interferon.
1981,
Pubmed Yip,
Molecular weight of human gamma interferon is similar to that of other human interferons.
1982,
Pubmed
