Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
1982 Jan 01;18:953-8. doi: 10.1002/j.1460-2075.1982.tb01277.x.
Show Gene links
Show Anatomy links
Cloning and structure of the human immune interferon-gamma chromosomal gene.
Taya Y, Devos R, Tavernier J, Cheroutre H, Engler G, Fiers W.
???displayArticle.abstract???
Two clones containing the human immune interferon-gamma (IFN-gamma) chromosomal gene were isolated from a human DNA library present in lambda Charon4A phage. DNA from these clones specified biologically active interferon upon injection into the nuclei of Xenopus laevis oocytes. Analysis of the clones revealed that they were derived from the same chromosomal segment. Restriction fragments that hybridized with 32P-labeled cDNA probes were subcloned into plasmids and the complete sequence of the IFN-gamma gene was determined. Unlike IFNs-alpha and -beta, IFN-gamma does contain introns. Their presence was also revealed by electron microscopy. It is intriguing that the smallest of the three introns is located just in the middle of the Glu-Glu sequence which is conserved among all three forms of interferon at approximately the same position. The promoter region was found to contain a prototype TATA box, many palindromic structures and several repeating sequences and two symmetrical structures. Particularly interesting was the existence of two sequences homologous to those present in the chicken albumin and the human IFN-beta gene promoter region. A sequence GTGTTG common to several other genes was found in the region approximately 10 nucleotides downstream from the polyadenylation site.
Benoist,
The ovalbumin gene-sequence of putative control regions.
1980, Pubmed
Benoist,
The ovalbumin gene-sequence of putative control regions.
1980,
Pubmed Benton,
Screening lambdagt recombinant clones by hybridization to single plaques in situ.
1977,
Pubmed Breathnach,
Ovalbumin gene: evidence for a leader sequence in mRNA and DNA sequences at the exon-intron boundaries.
1978,
Pubmed Breathnach,
Organization and expression of eucaryotic split genes coding for proteins.
1981,
Pubmed Degrave,
Nucleotide sequence of the chromosomal gene for human fibroblast (beta 1) interferon and of the flanking regions.
1981,
Pubmed Devos,
Molecular cloning of human immune interferon cDNA and its expression in eukaryotic cells.
1982,
Pubmed
,
Xenbase de Wet,
Restriction maps for twenty-one Charon vector phages.
1980,
Pubmed Epstein,
Interferon-gamma: success, structure and speculation.
1982,
Pubmed Fiers,
Complete nucleotide sequence of SV40 DNA.
1978,
Pubmed Gray,
Expression of human immune interferon cDNA in E. coli and monkey cells.
1982,
Pubmed Houghton,
The absence of introns within a human fibroblast interferon gene.
1981,
Pubmed Lawn,
DNA sequence of a major human leukocyte interferon gene.
1981,
Pubmed Lawn,
Human fibroblast interferon gene lacks introns.
1981,
Pubmed Lawn,
The isolation and characterization of linked delta- and beta-globin genes from a cloned library of human DNA.
1978,
Pubmed Lerner,
Are snRNPs involved in splicing?
1980,
Pubmed Maniatis,
The isolation of structural genes from libraries of eucaryotic DNA.
1978,
Pubmed Maxam,
Sequencing end-labeled DNA with base-specific chemical cleavages.
1980,
Pubmed Mount,
Sequence of U1 RNA from Drosophila melanogaster: implications for U1 secondary structure and possible involvement in splicing.
1981,
Pubmed Nagata,
The structure of one of the eight or more distinct chromosomal genes for human interferon-alpha.
1980,
Pubmed Remaut,
Plasmid vectors for high-efficiency expression controlled by the PL promoter of coliphage lambda.
1981,
Pubmed Smith,
A simple method for DNA restriction site mapping.
1976,
Pubmed Soberon,
Construction and characterization of new cloning vehicles. IV. Deletion derivatives of pBR322 and pBR325.
1980,
Pubmed Southern,
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
1975,
Pubmed Tavernier,
Evidence for a unique human fibroblast interferon (IFN-beta 1) chromosomal gene, devoid of intervening sequences.
1981,
Pubmed van Ooyen,
Comparison of total sequence of a cloned rabbit beta-globin gene and its flanking regions with a homologous mouse sequence.
1979,
Pubmed
