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The direction of growth of differentiating neurones and myoblasts from frog embryos in an applied electric field.
Hinkle L, McCaig CD, Robinson KR.
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1. Disaggregated single neurones and myoblasts obtained from the neural tube and somites of Xenopus laevis embryos (stages 17-21) were cultured in the presence of steady small electric fields. 2. Neurites grew preferentially towards the negative pole, or cathode, in field strengths of 7-190 mV/mm. Many turned through considerable angles to do so. This effect disappeared below a threshold level of about 7 mV/mm. 3. Greater numbers of neurones sprouted neurites in cultures exposed to an electric field compared to control cultures. The difference could be as much as tenfold. The threshold level of this phenomenon was about 6-8 mV/mm. Other cell types such as pigment cells and fibroblasts were also stimulated to differentiate in culture by an electric field, although to a lesser extent than neurones. 4. Applied electric fields had no effect on the location of the origin of neurites on the cell body. 5. Spherical myoblasts cultured in applied electric fields (36-170 mV/mm) elongated with a bipolar axis of growth which was perpendicular to the electric field. The response was graded and disappeared at field strengths below 36 mV/mm. 6. It is suggested that in vivo, the direction of neural outgrowth from the neural tube and the strict spatial organization of somites might be under the control, in part, of endogenous electric fields. Possible sources of these are discussed.
Alvarado,
Sodium and chloride transport in tadpoles of the bullfrog Rana catesbeiana.
1970, Pubmed
Alvarado,
Sodium and chloride transport in tadpoles of the bullfrog Rana catesbeiana.
1970,
Pubmed Baker,
Cytoplasmic filaments and morphogenetic movement in the amphibian neural tube.
1967,
Pubmed Blackshaw,
Alterations in resting membrane properties during neural plate stages of development of the nervous system.
1976,
Pubmed Blackshaw,
Low resistance junctions between mesoderm cells during development of trunk muscles.
1976,
Pubmed
,
Xenbase Bray,
Model for membrane movements in the neural growth cone.
1973,
Pubmed Bray,
Surface movements during the growth of single explanted neurons.
1970,
Pubmed Bunge,
Fine structure of nerve fibers and growth cones of isolated sympathetic neurons in culture.
1973,
Pubmed Ebendal,
The relative roles of contact inhibition and contact guidance in orientation of axons extending on aligned collagen fibrils in vitro.
1976,
Pubmed Gundersen,
Neuronal chemotaxis: chick dorsal-root axons turn toward high concentrations of nerve growth factor.
1979,
Pubmed Hamilton,
The formation of somites in Xenopus.
1969,
Pubmed
,
Xenbase Jaffe,
An ultrasensitive vibrating probe for measuring steady extracellular currents.
1974,
Pubmed Jaffe,
Neurites grow faster towards the cathode than the anode in a steady field.
1979,
Pubmed Jaffe,
Electrical controls of development.
1977,
Pubmed Ludueña,
Cell locomotion, nerve elongation, and microfilaments.
1973,
Pubmed Messenger,
The function of the sodium pump during differentiation of amphibian embryonic neurones.
1979,
Pubmed
,
Xenbase Morrill,
The electropotential difference between the blastocoel and the external medium in the amphibian embryo: its similarity to the adult frog trans-skin potential.
1966,
Pubmed Poo,
Electrophoresis of concanavalin A receptors along embryonic muscle cell membrane.
1977,
Pubmed
,
Xenbase Poo,
Lateral electrophoresis and diffusion of Concanavalin A receptors in the membrane of embryonic muscle cell.
1978,
Pubmed
,
Xenbase Slack,
Intracellular and intercellular potentials in the early amphibian embryo.
1973,
Pubmed
,
Xenbase TAYLOR,
TRANSEPIDERMAL POTENTIAL DIFFERENCE: DEVELOPMENT IN ANURAN LARVAE.
1965,
Pubmed Yamada,
Ultrastructure and function of growth cones and axons of cultured nerve cells.
1971,
Pubmed
