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Membrane lipid heterogeneity associated with acetylcholine receptor particle aggregates in Xenopus embryonic muscle cells.
Bridgman PC, Nakajima Y.
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Filipin, digitonin, and saponin react with membrane cholesterol to produce unique membrane alterations (sterol-specific complexes) that are easily discernible in freeze-fracture replicas. We have treated both noninnervated and innervated Xenopus embryonic muscle cells in culture with these agents. Freeze-fracture of these treated muscle cells showed that most areas of the muscleplasma membrane contain sterol-specific complexes (19- to 40-nm protuberances and dimples with filipin, a scalloped appearance with digitonin, or an irregular, rough appearance with saponin). However, these complexes were virtually absent from membrane areas of junctional and nonjunctional aggregates of acetylcholine receptor particles. This result suggests that the membrane matrix of these aggregates is low in cholesterol and that this membrane lipid heterogeneity may be linked to the mechanisms involved in their formation and stabilization on muscle cells in culture.
Anderson,
Effects of innervation on the distribution of acetylcholine receptors on cultured muscle cells.
1977, Pubmed,
Xenbase
Anderson,
Effects of innervation on the distribution of acetylcholine receptors on cultured muscle cells.
1977,
Pubmed
,
Xenbase Anderson,
Lipids of ocular tissues. IX. The phospholipids of frog photoreceptor membranes.
1974,
Pubmed Anderson,
Nerve-induced and spontaneous redistribution of acetylcholine receptors on cultured muscle cells.
1977,
Pubmed
,
Xenbase Andrews,
Freeze-fracture evidence for the presence of cholesterol in particle-free patches of basal disks and the plasma membrane of retinal rod outer segments of mice and frogs.
1979,
Pubmed Ashworth,
Plasma membranes: phospholipid and sterol content.
1966,
Pubmed Axelrod,
Control of acetylcholine receptor mobility and distribution in cultured muscle membranes. A fluorescence study.
1978,
Pubmed Axelrod,
Lateral motion of fluorescently labeled acetylcholine receptors in membranes of developing muscle fibers.
1976,
Pubmed BANGHAM,
NEGATIVE STAINING OF PHOSPHOLIPIDS AND THEIR STRUCTURAL MODIFICATION BY SURFACE-ACTIVE AGENTS AS OBSERVED IN THE ELECTRON MICROSCOPE.
1964,
Pubmed Bloch,
Dispersal and reformation of acetylcholine receptor clusters of cultured rat myotubes treated with inhibitors of energy metabolism.
1979,
Pubmed Cherry,
Lateral segregation of proteins induced by cholesterol in bacteriorhodopsin-phospholipid vesicles.
1980,
Pubmed Cohen,
Clusters of intramembrane particles associated with binding sites for alpha-bungarotoxin in cultured chick myotubes.
1979,
Pubmed Demel,
The function of sterols in membranes.
1976,
Pubmed Elias,
Freeze-fracture identification of sterol-digitonin complexes in cell and liposome membranes.
1978,
Pubmed Fischbach,
The distribution of acetylcholine sensitivity over uninnervated and innervated muscle fibers grown in cell culture.
1973,
Pubmed Frank,
Early events in neuromuscular junction formation in vitro: induction of acetylcholine receptor clusters in the postsynaptic membrane and morphology of newly formed synapses.
1979,
Pubmed Gabr,
Substance Q: a smooth muscle contracting substance liberated from human plasma lipids by the action of heat.
1967,
Pubmed Hasty,
Freeze-fracture studies of the developing cell surface. II. Particle-free membrane blisters on glutaraldehyde-fixed corneal fibroblasts are artefacts.
1978,
Pubmed Heuser,
Organization of acetylcholine receptors in quick-frozen, deep-etched, and rotary-replicated Torpedo postsynaptic membrane.
1979,
Pubmed Kallai-Sanfacon,
Characterization of the lipid and polypeptide components of a tetrodotoxin binding membrane fraction from Electrophorus electricus.
1980,
Pubmed Kinsky,
Antibiotic interaction with model membranes.
1970,
Pubmed Michaelson,
Purified acetylcholine receptor: its reconstitution to a chemically excitable membrane.
1974,
Pubmed Montesano,
Absence of filipin-sterol complexes from large coated pits on the surface of culture cells.
1979,
Pubmed Norman,
Polyene antibiotic - sterol interaction.
1976,
Pubmed Orci,
Occurrence of low density lipoprotein receptors within large pits on the surface of human fibroblasts as demonstrated by freeze-etching.
1978,
Pubmed Pauli,
Freeze-fracture of monolayer cultures.
1977,
Pubmed Peng,
Development of the postsynaptic membrane in Xenopus neuromuscular cultures observed by freeze-fracture and thin-section electron microscopy.
1980,
Pubmed
,
Xenbase Peng,
Membrane particle aggregates in innervated and noninnervated cultures of Xenopus embryonic muscle cells.
1978,
Pubmed
,
Xenbase Popot,
Interaction of the acetylcholine (nicotinic) receptor protein from Torpedo marmorata electric organ with monolayers of pure lipids.
1978,
Pubmed Röhlich,
Oriented pattern of membrane-associated vesicles in fibroblasts.
1976,
Pubmed SPERRY,
QUANTITATIVE ISOLATION OF STEROLS.
1963,
Pubmed Sytkowski,
Development of acetylcholine receptor clusters on cultured muscle cells.
1973,
Pubmed Verkleij,
Freeze-etch electron microscopy of erythrocytes, Acholeplasma laidlawii cells and liposomal membranes after the action of filipin and amphotericin B.
1973,
Pubmed Warren,
Cholesterol is excluded from the phospholipid annulus surrounding an active calcium transport protein.
1975,
Pubmed Weldon,
Development of synaptic ultrastructure at neuromuscular contacts in an amphibian cell culture system.
1979,
Pubmed
,
Xenbase Williamson,
Ultrastructural localization and distribution of free cholesterol (3-beta-hydroxysterols) in tissues.
1969,
Pubmed Yee,
Clusters of intramembranous particles on cultured myotubes at sites that are highly sensitive to acetylcholine.
1978,
Pubmed
