Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Distribution of sequences common to the 25--28S-ribonucleic acid genes of Xenopus laevis and Neurospora crassa.
Cox RA, Thompson RD.
???displayArticle.abstract???
The extent of homology between the nucleotide sequence of L-rRNA (the major RNA component of the larger ribosomal subparticle) of a lower eukaryote (Neurospora crassa) and an amphibian (Xenopus laevis) was investigated by utilizing rDNA (DNA coding for rRNA) of X. laevis cloned in plasmids pMB9 and pML2, and rDNA of N. crassa cloned in bacteriophage lambda. Hybridization studies revealed that sequences common to both N. crassa and X. laevis L-rRNA comprise a total of approx. 1050 /+- 200 nucleotides. The thermal stability of the X. laevis rDNA.N. crassa L-rRNA hybrid was 5 degrees C lower than that of the X. laevis rDNA.X. laevis L-rRNA duplex, indicating the presence of fewer than 10% mismatches in homologous sequences. X. laevis rDNA was analysed by means of restriction endonucleases and hybridization with 125I-labelled N. crassa L-rRNA. Most (at least 95%) of the conserved sequences were present in a 3000-base-pair fragment produced by restriction with endonucleases HindIII and BamHI. This fragment, which includes the 3'-OH terminus of the L-rRNA-coding region, was used as an adaptor in the construction of a bacteriophage-lambda recombinant. One section of the recombinant phage terminating in a HindIII-specific site was obtained from bacteriophage lambda plac5 (after restriction with endonuclease HindIII). A second section terminating in a BamHi-specific site was obtained from bacteriophage lambda 540 (after restriction with endonuclease BamHI). These two parts were joined by means of the X. laevis rDNA fragment. Further analysis of cloned rDNA by means of restriction endonucleases confirmed that conserved sequences were widely distributed throughout the 3000-base-pair fragment produced by HindIII and BamHi endonucleases. A 3400-base-pair fragment of N. crassa rDNA cloned in a bacteriophage lambda [Cox & Peden (1979) Mol. Gen. Genet. 174, 17--24] was restricted with endonucleases. The products were hybridized with 125I-labelled X. laevis L-rRNA. Conserved sequences were shown to be distributed over a range of approx. 1600--2700 base-pairs. Hence, in neither X. laevis nor N. crassa L-rRNA can be conserved sequences from a single block; instead regions of high and low (or no) homology must be intermingled. Both N. crassa rDNA and X. laevis rDNA were found to hybridize with Drosophila melanogaster L-rDNA sequences. Those rDNA fragments with sequences common to X. laevis and N. crassa L-rRNA also hybridized with D. melanogaster L-rRNA probe. Thus the same set of conserved sequences may be present in all three species.
Allet,
Analysis of bacteriophage mu and lambda-mu hybrid DNAs by specific endonucleases.
1975, Pubmed
Allet,
Analysis of bacteriophage mu and lambda-mu hybrid DNAs by specific endonucleases.
1975,
Pubmed Bonner,
Reduction in the rate of DNA reassociation by sequence divergence.
1973,
Pubmed Boseley,
Mapping of the Xenopus laevis 5.8S rDNA by restriction and DNA sequencing.
1978,
Pubmed
,
Xenbase Breitmeyer,
Affinity labeling of specific regions of 23 S RNA by reaction of N-bromoacetyl-phenylalanyl-transfer RNA with Escherichia coli ribosomes.
1976,
Pubmed Clewell,
Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.
1969,
Pubmed Cox,
Structure and function of prokaryotic and eukaryotic ribosomes.
1977,
Pubmed Cox,
Re-activation of the peptidyltransferase centre of rabbit reticulocyte ribosomes after inactivation by exposure to low concentrations of magnesium ion.
1976,
Pubmed Cox,
A novel recombinant of phage lambda and a conserved 3000-base-pair fragment of Xenopus laevis ribosomal deoxyribonucleic acid produced by restriction with endonucleases Hind III/BamI [proceedings].
1978,
Pubmed
,
Xenbase Cox,
A study of the influence of magnesium ions on the conformation of ribosomal ribonucleic acid and on the stability of the larger subribosomal particle of rabbit reticulocytes.
1976,
Pubmed Cox,
A study of the organisation of the ribosomal ribonucleic acid gene cluster of Neurospora crassa by means of restriction endonuclease analysis and cloning in bacteriophage lambda.
1979,
Pubmed
,
Xenbase Cox,
Organization of the ribosomal ribonucleic acid gene cluster of Neurospora crassa by means of restriction endonucleases and cloning in bacteriophage lambda [proceedings].
1978,
Pubmed Cox,
Spectroscopic evidence for the uneven distribution of adenine and uracil residues in ribosomal ribonucleic acid of Drosophila melanogaster and of Plasmodium knowlesi and its possible evolutionary significance.
1976,
Pubmed Dawid,
A reinvestigation of 5' leads to 3' polarity in 40S ribosomal RNA precursor of Xenopus laevis.
1976,
Pubmed
,
Xenbase Denhardt,
A membrane-filter technique for the detection of complementary DNA.
1966,
Pubmed Erdmann,
Collection of published 5S and 5.8S RNA sequences and their precursors.
1979,
Pubmed Free,
Arrangement of the genes coding for ribosomal ribonucleic acids in Neurospora crassa.
1979,
Pubmed Gerbi,
Fine structure of ribosomal RNA. I. Conservation of homologous regions within ribosomal RNA of eukaryotes.
1976,
Pubmed Gillespie,
A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane.
1965,
Pubmed Glover,
A novel arrangement of the 18S and 28S sequences in a repeating unit of Drosophila melanogaster rDNA.
1977,
Pubmed Glover,
Characterization of six cloned DNAs from Drosophila melanogaster, including one that contains the genes for rRNA.
1975,
Pubmed Godwin,
Studies of the RNA and protein moieties of the larger subribosomal particle of rabbit reticulocytes.
1974,
Pubmed
,
Xenbase HORWITZ,
SUBSTRATES FOR CYTOCHEMICAL DEMONSTRATION OF ENZYME ACTIVITY. I. SOME SUBSTITUTED 3-INDOLYL-BETA-D-GLYCOPYRANOSIDES.
1964,
Pubmed Laskey,
Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitized film.
1977,
Pubmed Lava-Sanchez,
Base composition of ribosomal RNA and evolution.
1972,
Pubmed Loening,
Molecular weights of ribosomal RNA in relation to evolution.
1968,
Pubmed Maniatis,
Nucleotide sequence of the rightward operator of phage lambda.
1975,
Pubmed Martin,
Hybrid 80S monomers formed from subunits of ribosomes from protozoa, ungi, plants, and mammals.
1970,
Pubmed Murray,
Lambdoid phages that simplify the recovery of in vitro recombinants.
1977,
Pubmed Murray,
Manipulation of restriction targets in phage lambda to form receptor chromosomes for DNA fragments.
1974,
Pubmed Murray,
Phage lambda receptor chromosomes for DNA fragments made with restriction endonuclease III of Haemophilus influenzae and restriction endonuclease I of Escherichia coli.
1975,
Pubmed Pratt,
Dissociation of ribosomes from oocytes of Xenopus laevis into active subparticles.
1971,
Pubmed
,
Xenbase Sinclair,
Retention of common nucleotide sequences in the ribosomal deoxyribonucleic acid of eukaryotes and some of their physical characteristics.
1971,
Pubmed Southern,
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
1975,
Pubmed Wellauer,
Secondary structure maps of ribosomal RNA and DNA. I. Processing of Xenopus laevis ribosomal RNA and structure of single-stranded ribosomal DNA.
1974,
Pubmed
,
Xenbase
