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Purified DNA, from Xenopus erythrocytes, containing genes for 5S ribosomal RNA (58 DNA) is transcribed faithfully when it is injected into the nucleus of a Xenopus oocyte. Full length 5S RNA of the correct sequence is transcribed from the injected DNA, and the newly synthesized transcripts hybridize predominantly with the coding strand of 5S DNA. The majority of transcripts hybridize with the gene region as opposed to the spacer region of the coding strand. Accurate transcription starts within the first 3 hr and continues for up to 4 days after injection of 5S DNA. Radioactive 5S RNA is detected in as short a labeling time as 1 hr.
Bonner,
A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
1974, Pubmed
Bonner,
A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
1974,
Pubmed Brown,
The structure and evolution of ribosomal and 5S DNAs in Xenopus laevis and Xenopus mulleri.
1974,
Pubmed
,
Xenbase Brown,
The nucleotide sequence adjoining the 3' end of the genes coding for oocyte-type 5 S ribosomal RNA in Xenopus.
1976,
Pubmed
,
Xenbase Brown,
5 S DNAs of Xenopus laevis and Xenopus mulleri: evolution of a gene family.
1973,
Pubmed
,
Xenbase Brown,
Purification and some characteristics of 5S DNA from Xenopus laevis.
1971,
Pubmed
,
Xenbase Brown,
Gene linkage by RNA-DNA hybridization. I. Unique DNA sequences homologous to 4 s RNA, 5 s RNA and ribosomal RNA.
1968,
Pubmed
,
Xenbase Brownlee,
Sequence studies of the 5 S DNA of Xenopus laevis.
1974,
Pubmed
,
Xenbase Colman,
Synthesis of RNA in oocytes of Xenopus laevis during culture in vitro.
1974,
Pubmed
,
Xenbase Commerford,
Iodination of nucleic acids in vitro.
1971,
Pubmed Denis,
[Biochemical research on oogenesis. V. Comparison between somatic 5 S RNA and oocyte 5 S RNA in Xenopus laevis].
1972,
Pubmed
,
Xenbase Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase Ford,
Sequences of 5S ribosomal RNA from Xenopus mulleri and the evolution of 5S gene-coding sequences.
1976,
Pubmed
,
Xenbase Gurdon,
Injected nuclei in frog oocytes: fate, enlargement, and chromatin dispersal.
1976,
Pubmed
,
Xenbase Honjo,
Preferential transcription of Xenopus laevis ribosomal RNA in interspecies hybrids between Xenopus laevis and Xenopus mulleri.
1973,
Pubmed
,
Xenbase Mertz,
Purified DNAs are transcribed after microinjection into Xenopus oocytes.
1977,
Pubmed
,
Xenbase Parker,
Selective and accurate transcription of the Xenopus laevis 5S RNA genes in isolated chromatin by purified RNA polymerase III.
1977,
Pubmed
,
Xenbase Peacock,
Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels.
1968,
Pubmed Sharp,
Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.
1973,
Pubmed Wegnez,
Sequence heterogeneity of 5 S RNA in Xenopus laevis.
1972,
Pubmed
,
Xenbase
