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Relationship between interferon production and interferon messenger RNA synthesis in human fibroblasts.
Raj NB, Pitha PM.
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Poly(A) containing mRNA prepared from poly(rI)-poly(rC)-induced human fibroblasts stimulated [14C]leucine incorporation into protein in wheat germ cell-free extracts. For the translation of interferon mRNA into a biologically active product, the presence of spermine was essential. The protein synthesized in vitro fulfilled the criteria for human interferon--namely, its antiviral activity was species specific, and its activity was completely neutralized by antiserum to human fibroblast interferon. The amount of interferon synthesized in human fibroblasts induced by poly(rI)-poly(rC) (normal induction) and poly(rI)-poly(rC) in the presence of cycloheximide (superinduction) was compared to the amount of translatable interferon mRNA both in the wheat germ cell-free system and the Xenopus oöcyte system. Although the production of interferon after the termination of transcription by actinomycin D was markedly increased in superinduced cells, the measurable amount of interferon mRNA as assayed in the oöcyte system was only slightly higher in superinduced cells than in cells induced with poly(rI)-poly(rC) alone. When compared in the wheat germ cell-free system, however, the translational product of mRNA preparation from cells induced with poly(rI)-poly(rC) alone was inactive while that from superinduced cells was active.
Both,
Methylation-dependent translation of viral messenger RNAs in vitro.
1975, Pubmed
Both,
Methylation-dependent translation of viral messenger RNAs in vitro.
1975,
Pubmed Field,
Inducers of interferon and host resistance. II. Multistranded synthetic polynucleotide complexes.
1967,
Pubmed Havell,
Two antigenically distinct species of human interferon.
1975,
Pubmed Levy-Koenig,
Immunology of interferons. I. Immune response to protective and nonprotective interferons.
1970,
Pubmed Palmiter,
Regulation of protein synthesis in chick oviduct. 3. Mechanism of ovalbumin "superinduction" by actinomycin D.
1973,
Pubmed Pestka,
Cell-free synthesis of human interferon.
1975,
Pubmed Reynolds,
Molecular weight study of human fibroblast interferon.
1975,
Pubmed Reynolds,
Interferon activity produced by translation of human interferon messenger RNA in cell-free ribosomal systems and in Xenopus oöcytes.
1975,
Pubmed
,
Xenbase Reynolds,
The induction of interferon and its messenger RNA in human fibroblasts.
1974,
Pubmed Roberts,
Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ.
1973,
Pubmed Sehgal,
An evaluation of messenger RNA competition in the shutoff of human interferon production.
1976,
Pubmed Sehgal,
Regulation of human interferon production. I. Superinduction by 5, 6-dichloro-1-beta-D-ribofuranosylbenzimidazole.
1976,
Pubmed Sehgal,
On the mechanism of enhancement of human interferon production by actinomycin D and cycloheximide.
1976,
Pubmed Steinberg,
"Superinduction" of tyrosine aminotransferase by actinomycin D: a reevaluation.
1975,
Pubmed Tan,
Regulation of cellular interferon production: enhancement by antimetabolites.
1970,
Pubmed Tan,
The somatic cell genetics of human interferon: assignment of human interferon loci to chromosomes 2 and 5.
1974,
Pubmed Thang,
Biosynthesis of mouse interferon by translation of its messenger RNA in a cell-free system.
1975,
Pubmed Tomkins,
Further evidence for posttranscriptional control of inducible tyrosine aminotransferase synthesis in cultured hepatoma cells.
1972,
Pubmed Vilcek,
Stabilization of interferon messenger RNA activity by treatment of cells with metabolic inhibitors and lowering of the incubation temperature.
1973,
Pubmed
