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The possibility of using DNA-injected Xenopus laevis oocytes and eggs for studying the control of transcription in eukaryotes has been investigated. When purified DNA of simian virus 40 (SV40) is injected into Xenopus laevis oocytes, tritiated RNA precursors are incorporated into DNase-I-resistant, RNase-A- and alkali-sensitive material that hybridizes specifically to SV40 DNA. This viral transcription continues for at least 5 days and occurs only when the injected DNA is directed to the nucleus of the oocyte. The quantity of SV40-specific RNA produced is roughly proportional to the amount of DNA injected; above 1 ng per oocyte, most of the nonribosomal RNA made in successfully injected oocytes is virus-specific. Transcription also occurs, although at a lower efficiency, after injection of the DNA into unfertilized eggs. The DNAs of adenovirus 5, cloned Drosophila melanogaster histone genes, and even bacteriophage phiX174 replicative form, bacteriophage phi80plac, and the ColE1 plasmid are also transcribed after injection into oocytes or eggs.
Chambon,
Eukaryotic nuclear RNA polymerases.
1975,
Pubmed
,
Xenbase Clewell,
Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.
1972,
Pubmed Colman,
Transcription of DNAs of known sequence after injection into eggs and oocytes of Xenopus laevis.
1975,
Pubmed
,
Xenbase Colman,
Synthesis of RNA in oocytes of Xenopus laevis during culture in vitro.
1974,
Pubmed
,
Xenbase Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase Gurdon,
Injected nuclei in frog oocytes: fate, enlargement, and chromatin dispersal.
1976,
Pubmed
,
Xenbase Gurdon,
Injected nuclei in frog oocytes:RNA synthesis and protein exchange.
1976,
Pubmed
,
Xenbase Gurdon,
Use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells.
1971,
Pubmed
,
Xenbase Gurdon,
The replication of purified DNA introduced into living egg cytoplasm.
1969,
Pubmed Gurdon,
Injected nuclei in frog oocytes provide a living cell system for the study of transcriptional control.
1976,
Pubmed
,
Xenbase Jackson,
Inhibition by -amanitin of simian virus 40-specific ribonucleic acid synthesis in nuclei of infected monkey cells.
1972,
Pubmed Katz,
Stimulation by cyclic adenosine monophosphate of plasmid deoxyribonucleic acid replication and catabolite repression of the plasmid deoxyribonucleic acid-protein relaxation complex.
1973,
Pubmed Laskey,
Induction of polyoma DNA synthesis by injection into frog-egg cytoplasm.
1973,
Pubmed
,
Xenbase Lobban,
Enzymatic end-to end joining of DNA molecules.
1973,
Pubmed Mertz,
Isolation and characterization of individual clones of simian virus 40 mutants containing deletions duplications and insertions in their DNA.
1975,
Pubmed Mertz,
Defective simian virus 40 genomes: isolation and growth of individual clones.
1974,
Pubmed Sedat,
Purification of Escherichia coli pulse-labeled RNA by benzoylated DEAE-cellulose chromatography.
1969,
Pubmed Udvardy,
Transcription of specific genes in isolated nuclei from HeLa cells in vitro.
1976,
Pubmed Yamamoto,
Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification.
1970,
Pubmed
