Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Translation and stability of human globin mRNA in Xenopus oocytes.
Maniatis GM, Ramirez F, Cann A, Marks PA, Bank A.
???displayArticle.abstract???
Human globin messenger RNA (mRNA) prepared from erythroid cells of patients with sickle cell anemia has been translated in Xenopus laevis oocytes. Addition of hemin to the injected mRNA causes total globin synthesis to increase and the ratio of alpha- to betas-globin synthesis (alpha/betas ratio) to approach unity. To determine the effect of the length of the poly-(A) segment on human globin mRNA stability, 10 S globin mRNA was fractionated into poly-(A)-poor fractions by oligo (dT)-cellulose column chromatography. When oocytes are injected with each of these fractions, translation of the poly-(A)-rich globin mRNA is sustained for a longer period than that of the poly-(A)-poor mRNA. Regardless of the mRNA fraction injected, the alpha/betas ratio of the synthesized globin decreases as the injected oocytes are incubated for longer periods. The results indicate that in frog oocytes poly-(A)-rich mRNA has greater translational stability than poly-(A)-poor mRNA, AND beta-mRNA has greater stability than alpha-mRNA with comparable poly-(A) content.
Adamson,
Involvement of hemin, a stimulatory fraction from ribosomes and a protein synthesis inhibitor in the regulation of hemoglobin synthesis.
1972, Pubmed
Adamson,
Involvement of hemin, a stimulatory fraction from ribosomes and a protein synthesis inhibitor in the regulation of hemoglobin synthesis.
1972,
Pubmed Allende,
The degradation of ribonucleic acids injected into Xenopus laevis oocytes.
1974,
Pubmed
,
Xenbase Bank,
Regulation of globin synthesis in the thalassemias.
1974,
Pubmed Beuzard,
The effects of hemin and double-stranded RNA on alpha and beta globin synthesis in reticulocyte and Krebs II ascites cell-free systems and the relationship of these effects to an initiation factor preparation.
1974,
Pubmed Beuzard,
Effect of hemin on the synthesis of hemoglobin and other proteins in mammalian cells.
1973,
Pubmed Cann,
Polyadenylate sequences and biologic activity of human globin messenger ribonucleic acid.
1974,
Pubmed Chan,
Translation of ovalbumin mRNA in Xenopus laevis oocytes. Characterization of the system and effects of estrogen on injected mRNA populations.
1976,
Pubmed
,
Xenbase Clegg,
Abnormal human haemoglobins. Separation and characterization of the alpha and beta chains by chromatography, and the determination of two new variants, hb Chesapeak and hb J (Bangkok).
1966,
Pubmed FANELLI,
Studies on the structure of hemoglobin. I. Physicochemical properties of human globin.
1958,
Pubmed Giglioni,
Translational control of globin synthesis by haemin in Xenopus oocytes.
1973,
Pubmed
,
Xenbase Gorski,
Size heterogeneity of polyadenylate sequences in mouse globin messenger RNA.
1974,
Pubmed Grayzel,
The stimulation of globin synthesis by heme.
1966,
Pubmed Gurdon,
Message stability in injected frog oocytes: long life of mammalian alpha and beta globin messages.
1973,
Pubmed
,
Xenbase Gurdon,
Use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells.
1971,
Pubmed
,
Xenbase Hieter,
Stabilization of an RNA molecule by 3'-terminal poly (A)-induced inhibition of RNase activity.
1976,
Pubmed Housman,
Quantitative deficiency of chain-specific globin messenger ribonucleic acids in the thalassemia syndromes.
1973,
Pubmed Huez,
Role of the polyadenylate segment in the translation of globin messenger RNA in Xenopus oocytes.
1974,
Pubmed
,
Xenbase Lane,
Rabbit haemoglobin synthesis in frog cells: the translation of reticulocyte 9 s RNA in frog oocytes.
1971,
Pubmed Lane,
Control of translation of globin mRNA in embryonic cells.
1974,
Pubmed Lodish,
Alpha and beta globin messenger ribonucleic acid. Different amounts and rates of initiation of translation.
1971,
Pubmed Mathews,
Specificity of the control of protein synthesis by haemin.
1973,
Pubmed Maxwell,
Hemin control of globin synthesis: an assay for the inhibitor formed in the absence of hemin and some characteristics of its formation.
1971,
Pubmed Metafora,
Increased efficiency of exogenous messenger RNA translation in a Krebs ascites cell lysate.
1972,
Pubmed Nienhuis,
Isolation and translation of hemoglobin messenger RNA from thalassemia, sickle cell anemia, and normal human reticulocytes.
1971,
Pubmed Nudel,
Globin mRNA species containing poly(A) segments of different lengths. Their functional stability in Xenopus oocytes.
1976,
Pubmed
,
Xenbase Sheiness,
Polyadenylic acid segment in mRNA becomes shorter with age.
1973,
Pubmed Sheiness,
Possible relationship of poly(A) shortening to mRNA turnover.
1975,
Pubmed Sippel,
Translational properties of rabbit globin mRNA after specific removal of poly(A) with ribonuclease H.
1974,
Pubmed Soreq,
In vitro translation of polyadenylic acid-free rabbit globin messenger RNA.
1974,
Pubmed Stevens,
Specific IgG mRNA molecules from myeloma cells in heterogeneous nuclear and cytoplasmic RNA containing poly-A.
1972,
Pubmed
,
Xenbase Williamson,
Translation of mouse globin messenger ribonucleic acid from which the poly(adenylic acid) sequence has been removed.
1974,
Pubmed Williamson,
Globin messenger sequences in high molecular weight RNA from embryonic mouse liver.
1973,
Pubmed
,
Xenbase Yip,
Translation of messenger ribonucleic acid from isolated pancreatic islets and human insulinomas.
1975,
Pubmed
,
Xenbase Zucker,
The synthesis of globin dimers by a reticulocyte cell-free system.
1967,
Pubmed
