Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Chromosoma
1975 Jan 01;504:349-69. doi: 10.1007/bf00327074.
Show Gene links
Show Anatomy links
In situ hybridization of ribosomal DNA labelled with 125iodine to metaphase and lampbrush chromosomes from newts.
Hennen S, Mizuno S, Macgregor HC.
???displayArticle.abstract???
Methods are described for in situ hybridization of ribosomal DNA from Xenopus laevis, labelled in vitro with 125iodine, to mitotic and lampbrush chromosomes from Triturus cristatus carnifex. The hybridization reaction was carried out in a mixture containing 50% formamide, 4 X SSC, 0.1 M KI, at 37 degrees C, or in 2 X SSC, 0.1 M KI at 65 degrees C. Autoradiographs of mitotic metaphases from 2 males showed labelling over the middle of the short arm of one chromosome IX in each metaphase. In some cases, a region near the end of a longer chromosome was also labelled. In a lampbrush preparations, labelling was confined to a region identified as about 53 units, near the middle of the short arm of both halves of bivalent IX. The usefulness of the technique and the significance of the labelling of only 1 of the 2 chromosomes IX in mitotic preparations are discussed.
Ando,
A nuclease specific for heat-denatured DNA in isolated from a product of Aspergillus oryzae.
1966, Pubmed
Ando,
A nuclease specific for heat-denatured DNA in isolated from a product of Aspergillus oryzae.
1966,
Pubmed Barsacchi,
Chromosomal localization of repetitive DNA in the newt, Triturus.
1972,
Pubmed Brown,
Specific gene amplification in oocytes. Oocyte nuclei contain extrachromosomal replicas of the genes for ribosomal RNA.
1968,
Pubmed Brown,
Gene linkage by RNA-DNA hybridization. I. Unique DNA sequences homologous to 4 s RNA, 5 s RNA and ribosomal RNA.
1968,
Pubmed
,
Xenbase Callan,
Chromosomes and nucleoli of the axolotl, Ambystoma mexicanum.
1966,
Pubmed Clarkson,
Clustering of transfer RNA genes of Xenopus laevis.
1973,
Pubmed
,
Xenbase Commerford,
Iodination of nucleic acids in vitro.
1971,
Pubmed CONGER,
A quick-freeze method for making smear slides permanent.
1953,
Pubmed Denhardt,
A membrane-filter technique for the detection of complementary DNA.
1966,
Pubmed Eckhardt,
Satellite DNA associated with heterochromatin in Rhynchosciara.
1971,
Pubmed Evans,
Localization of amplified ribosomal DNA in the oocyte of Xenopus laevis.
1968,
Pubmed
,
Xenbase Gall,
Formation and detection of RNA-DNA hybrid molecules in cytological preparations.
1969,
Pubmed
,
Xenbase Gall,
Reptitive DNA sequences in drosophila.
1971,
Pubmed Gall,
Differential synthesis of the genes for ribosomal RNA during amphibian oögenesis.
1968,
Pubmed Grigliatti,
The localization of transfer RNA5Lys genes in Drosophila melanogaster.
1974,
Pubmed Hennig,
Repeated sequences in the DNA of Drosophila and their localization in giant chromosomes.
1970,
Pubmed Jones,
Chromosomal and nuclear location of mouse satellite DNA in individual cells.
1970,
Pubmed Jones,
Localisation of reiterated nucleotide sequences in Drosophila and mouse by in situ hybridisation of complementary RNA.
1970,
Pubmed Kezer,
The nucleolar organizer of Plethodon cinereus cinereus (Green). II. The lampbrush nucleolar organizer.
1973,
Pubmed Lacroix,
[Demonstration on lampbrush chromosomes of Pleurodeles poireti Gervais, urodele amphibia, of a structure linked with sex, identifying the sexual bivalent and labelling the chromosome W].
1970,
Pubmed Macgregor,
The nucleolus and its genes in amphibian oogenesis.
1972,
Pubmed
,
Xenbase Macgregor,
Observations on centromeric heterochromatin and satellite DNA in salamanders of the genus Plethodon.
1973,
Pubmed Macgregor,
Amplification, polytenisation, and nucleolus organisers.
1973,
Pubmed
,
Xenbase Macgregor,
The chromosomal localization of a heavy satellite DNA in the testis of Plethodon c. cinereus.
1971,
Pubmed Macgregor,
The nucleolar organizer of Plethodon cinereus cinereus (Green). I. Location of the nucleolar organizer by in situ nucleic acid hybridization.
1973,
Pubmed
,
Xenbase Mancino,
Chromosomal heteromorphism and female heterogamety in the marbled newt Triturus marmoratus (Latreille, 1800).
1971,
Pubmed Miller,
Mutations affecting the size of the nucleolus in Xenopus laevis.
1970,
Pubmed
,
Xenbase Mizuno,
Chromosomes, DNA sequences, and evolution in salamanders of the genus Plethodon.
1974,
Pubmed Pardue,
Chromosomal localization of mouse satellite DNA.
1970,
Pubmed Pardue,
Location of the genes for 5S ribosomal RNA in Xenopus laevis.
1973,
Pubmed
,
Xenbase Pilone,
Chromosome location of the genes for 28S, 18S and 5S ribosomal RNA in Triturus marmoratus (Amphibia Urodela).
1974,
Pubmed
,
Xenbase Prensky,
The use of iodinated RNA for gene localization.
1973,
Pubmed RITOSSA,
LOCALIZATION OF DNA COMPLEMENTARY TO RIBOSOMAL RNA IN THE NUCLEOLUS ORGANIZER REGION OF DROSOPHILA MELANOGASTER.
1965,
Pubmed Sinclair,
Variation in rDNA redundancy level and nucleolar organizer length in normal and variant lines of the Mexican axolotl.
1974,
Pubmed
,
Xenbase Wallace,
Ribosomal cistrons and the nucleolar organizer.
1966,
Pubmed Wen,
Multiple gene sites for 5S and 18 plus 28S RNA on chromosomes of Glyptotendipes barbipes (Staeger).
1974,
Pubmed Wimber,
Localization of 5S RNA genes on Drosophila chromosomes by RNA-DNA hybridization.
1970,
Pubmed
