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DNA containing the multiple genes for 5S RNA has been isolated from the genome of Xenopus laevis. Whereas 5S RNA is about 57% G + C, the 5S DNA has a base composition of about 33-35% GC and consists of two alternating regions that differ in base composition by at least 20% GC. A denaturation map of 5S DNA analyzed by electron microscopy demonstrates that the repeating pattern is regular and each repeating unit has a mass of about 500,000 daltons. If one gene for 5S RNA (84,000 daltons native) were present in each repeat, it should comprise about 16.8% of 5S DNA. This arrangement is confirmed, since 6.8% of pure 5S DNA (13.6% of its base pairs) hybridized with 5S RNA. The remaining 83% of each repeating unit is considered to be "spacer" DNA. The 5S RNA hybridizes with about 0.05% of the bulk DNA of X. laevis, so that 5S DNA comprises about 0.7% of the total nuclear DNA. This is equivalent to about 24,000 repeating units for each haploid complement of DNA. These repeats are highly clustered; as many as 86 have been visualized along a single DNA molecule.
Aloni,
Studies of fractionated HeLa cell metaphase chromosomes. II. chromosomal distribution of sites for transfer RNA and 5 s RNA.
1971, Pubmed
Aloni,
Studies of fractionated HeLa cell metaphase chromosomes. II. chromosomal distribution of sites for transfer RNA and 5 s RNA.
1971,
Pubmed Birnstiel,
Localization of the ribosomal DNA complements in the nucleolar organizer region of Xenopus laevis.
1966,
Pubmed
,
Xenbase Brown,
Synthesis and accumulation of low molecular weight RNA during embryogenesis of Xenopus laevis.
1966,
Pubmed
,
Xenbase Brown,
Gene linkage by RNA-DNA hybridization. I. Unique DNA sequences homologous to 4 s RNA, 5 s RNA and ribosomal RNA.
1968,
Pubmed
,
Xenbase Corneo,
Different satellite deoxyribonucleic acids of guinea pig and ox.
1970,
Pubmed Corneo,
Isolation and characterization of mouse and guinea pig satellite deoxyribonucleic acids.
1968,
Pubmed Corneo,
Repeated sequences in human DNA.
1970,
Pubmed Dawid,
Composition and structure of chromosomal and amplified ribosomal DNA's of Xenopus laevis.
1970,
Pubmed
,
Xenbase Freifelder,
Molecular weights of coliphages and coliphage DNA. IV. Molecular weights of DNA from bacteriophages T4, T5 and T7 and the general problem of determination of M.
1970,
Pubmed Hatlen,
Oligonucleotide pattern after pancreatic ribonuclease digestion and the 3' and 5' termini of 5S ribonucleic acid from HeLa cells.
1969,
Pubmed Kersten,
Physicochemical properties of complexes between deoxyribonucleic acid and antibiotics which affect ribonucleic acid synthesis (actinomycin, daunomycin, cinerubin, nogalamycin, chormomycin, mithramycin, and olivomycin).
1966,
Pubmed Kirk,
Effect of methylation of cytosine residues on the buoyant density of DNA in caesium chloride solution.
1967,
Pubmed Mairy,
[Biochemical studies on oogenesis. I. RNA synthesis and accumulation during oogenesis of the South African toad Xenopus laevis].
1971,
Pubmed
,
Xenbase McConaughy,
Nucleic acid reassociation in formamide.
1969,
Pubmed Perry,
Persistent synthesis of 5S RNA when production of 28S and 18S ribosomal RNA is inhibited by low doses of actinomycin D.
1968,
Pubmed SCHILDKRAUT,
Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl.
1962,
Pubmed Smith,
The introduction of radioisotopes into RNA by methylation in vitro.
1967,
Pubmed Wensink,
Denaturation map of the ribosomal DNA of Xenopus laevis.
1971,
Pubmed
,
Xenbase Wimber,
Localization of 5S RNA genes on Drosophila chromosomes by RNA-DNA hybridization.
1970,
Pubmed
