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A comparative study in vivo and in vitro of the ability of ribosomes from Xenopus liver and ovary to incorporate L-[U-14C]leucine.
Ford PJ.
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1. A system for the incorporation in vitro of amino acids into protein is described for the South African clawed toad (Xenopus laevis laevis Daudin). 2. The incorporation of l-[U-(14)C]leucine by Xenopus-liver microsomes is very much greater per mg. of microsomal RNA than the incorporation by ovary microsomes. 3. The incorporation by Xenopus-liver and -ovary polysomes is approximately the same when expressed per mg. of polysomal RNA. 4. It was predicted from the above results that ovary microsomes should contain a ribosomal fraction inactive in protein synthesis. This was shown to be the case by a labelling experiment in vivo with l-[U-(14)C]leucine. 5. The labelling experiment in vivo also showed that the active polysomal fraction in ovary is associated with membranes and is liberated by treatment with deoxycholate; this is also true of liver microsomes in vivo. 6. The results are discussed in relation to previous work on the synthesis of proteins by amphibian ovarian tissue, and on the role of bound and free ribosomes in protein synthesis.
BROWN,
RNA SYNTHESIS DURING THE DEVELOPMENT OF XENOPUS LAEVIS, THE SOUTH AFRICAN CLAWED TOAD.
1964, Pubmed,
Xenbase
BROWN,
RNA SYNTHESIS DURING THE DEVELOPMENT OF XENOPUS LAEVIS, THE SOUTH AFRICAN CLAWED TOAD.
1964,
Pubmed
,
Xenbase Campbell,
Studies on the protein-synthesizing activity of the ribosomes of rat liver. The activity of free polysomes.
1965,
Pubmed Campbell,
Studies on the role of the morphological constituents of the microsome fraction from rat liver in protein synthesis.
1964,
Pubmed EARL,
THE ISOLATION AND PROPERTIES OF CARDIAC RIBOSOMES AND POLYSOMES.
1965,
Pubmed GANOZA,
SYNTHESIS OF SERUM PROTEINS BY A CELL-FREE SYSTEM FROM RAT LIVER.
1965,
Pubmed GIERER,
Function of aggregated reticulocyte ribosomes in protein synthesis.
1963,
Pubmed GROSS,
THE IMMEDIACY OF GENOMIC CONTROL DURING EARLY DEVELOPMENT.
1964,
Pubmed Hall,
High-efficiency liquid-scintillation counting of 14C-labelled material in aqueous solution and determination of specific activity of labelled proteins.
1965,
Pubmed HENSHAW,
PROTEIN SYNTHESIS BY FREE AND BOUND RAT LIVER RIBOSOMES IN VIVO AND IN VITRO.
1963,
Pubmed HOAGLAND,
A soluble ribonucleic acid intermediate in protein synthesis.
1958,
Pubmed HULTIN,
Activation of ribosomes in sea urchin eggs in response to fertilization.
1961,
Pubmed HULTIN,
ON THE MECHANISM OF RIBOSOMAL ACTIVATION IN NEWLY FERTILIZED SEA URCHIN EGGS.
1964,
Pubmed KEMP,
Electron microscopy of growing oocytes of Rana pipiens.
1956,
Pubmed KEMP,
Incorporation of radioactive glycine into proteins of frog oocytes.
1955,
Pubmed KOHNE,
THE ISOLATION OF RIBOSOMES FROM EGGS AND EMBRYOS OF RANA PIPIENS.
1965,
Pubmed LOWRY,
Protein measurement with the Folin phenol reagent.
1951,
Pubmed Marks,
PROTEIN SYNTHESIS IN ERYTHROID CELLS, I. RETICULOCYTE RIBOSOMES ACTIVE IN STIMULATING AMINO ACID INCORPORATION.
1962,
Pubmed Munro,
Studies on the nature of polysomes.
1964,
Pubmed PALADE,
Liver microsomes; an integrated morphological and biochemical study.
1956,
Pubmed PALADE,
Pancreatic microsomes; an integrated morphological and biochemical study.
1956,
Pubmed PORTER,
Electron microscopy of basophilic components of cytoplasm.
1954,
Pubmed PRESCOTT,
Nuclear function and nuclear-cytoplasmic interactions.
1960,
Pubmed SIEKEVITZ,
A cytochemical study on the pancreas of the guinea pig. 5. In vivo incorporation of leucine-1-C14 into the chymotrypsinogen of various cell fractions.
1960,
Pubmed SLAUTTERBACK,
The development of the cnidoblasts of Hydra; an electron microscope study of cell differentiation.
1959,
Pubmed Warner,
Electron Microscope Studies of Ribosomal Clusters Synthesizing Hemoglobin.
1962,
Pubmed WARNER,
A multiple ribosomal structure in protein synthesis.
1963,
Pubmed WETTSTEIN,
Ribosomal aggregate engaged in protein synthesis: characterization of the ergosome.
1963,
Pubmed
