Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-34460
Pflugers Arch 2006 Oct 01;4531:117-23. doi: 10.1007/s00424-006-0125-y.
Show Gene links Show Anatomy links

Automated fast perfusion of Xenopus oocytes for drug screening.

Baburin I, Beyl S, Hering S.


???displayArticle.abstract???
Fast ('concentration jump') applications of neurotransmitters are crucial for screening studies on ligand-gated ion channels. In this paper, we describe a method for automated fast perfusion of neurotransmitters (or drugs) during two-microelectrode voltage-clamp experiments on Xenopus oocytes. The oocytes are placed in a small bath chamber that is covered by a glass plate with two channels for the microelectrodes that are surrounded by a quartz funnel serving as a reservoir for test solutions. The oocytes are perfused in a vertical direction via the two channels in the plate. Automation of compound delivery is accomplished by means of a programmable pipetting workstation. A mean rise time for 10-90% current increase through muscle-type nACh channels of 55.0+/-1.3 ms (30 muM acetylcholine) was estimated. Automation, fast perfusion rates, and economical use of compounds ( approximately 100 mul/data point) make the system suitable for screening studies on ligand- and voltage-gated ion channels.

???displayArticle.pubmedLink??? 16953425
???displayArticle.pmcLink??? PMC3189690
???displayArticle.link??? Pflugers Arch
???displayArticle.grants??? [+]


References [+] :
Dascal, The use of Xenopus oocytes for the study of ion channels. 1987, Pubmed, Xenbase