Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Cell Biol
2006 Dec 01;2624:9291-301. doi: 10.1128/MCB.01183-06.
Show Gene links
Show Anatomy links
Evolutionarily conserved role of nucleostemin: controlling proliferation of stem/progenitor cells during early vertebrate development.
Beekman C, Nichane M, De Clercq S, Maetens M, Floss T, Wurst W, Bellefroid E, Marine JC.
???displayArticle.abstract??? Nucleostemin (NS) is a putative GTPase expressed preferentially in the nucleoli of neuronal and embryonic stem cells and several cancer cell lines. Transfection and knockdown studies indicated that NS controls the proliferation of these cells by interacting with the p53 tumor suppressor protein and regulating its activity. To assess the physiological role of NS in vivo, we generated a mutant mouse line with a specific gene trap event that inactivates the NS allele. The corresponding NS(-/-) embryos died around embryonic day 4. Analyses of NS mutant blastocysts indicated that NS is not required to maintain pluripotency, nucleolar integrity, or survival of the embryonic stem cells. However, the homozygous mutant blastocysts failed to enter S phase even in the absence of functional p53. Haploid insufficiency of NS in mouse embryonic fibroblasts leads to decreased cell proliferation. NS also functions in early amphibian development to control cell proliferation of neural progenitor cells. Our results show that NS has a unique ability, derived from an ancestral function, to control the proliferation rate of stem/progenitor cells in vivo independently of p53.
Artus,
The cell cycle of early mammalian embryos: lessons from genetic mouse models.
2006, Pubmed
Artus,
The cell cycle of early mammalian embryos: lessons from genetic mouse models.
2006,
Pubmed Baserga,
Inhibition of DNA synthesis in Ehrlich ascites cells by actinomycin D. II. The presynthetic block in the cell cycle.
1965,
Pubmed Bassler,
Identification of a 60S preribosomal particle that is closely linked to nuclear export.
2001,
Pubmed Bellmeyer,
The protooncogene c-myc is an essential regulator of neural crest formation in xenopus.
2003,
Pubmed
,
Xenbase Bernardi,
The nucleolus: at the stem of immortality.
2003,
Pubmed Chitnis,
Primary neurogenesis in Xenopus embryos regulated by a homologue of the Drosophila neurogenic gene Delta.
1995,
Pubmed
,
Xenbase Colombo,
Nucleophosmin regulates the stability and transcriptional activity of p53.
2002,
Pubmed Colombo,
Delocalization and destabilization of the Arf tumor suppressor by the leukemia-associated NPM mutant.
2006,
Pubmed Colombo,
Nucleophosmin is required for DNA integrity and p19Arf protein stability.
2005,
Pubmed Dai,
Inhibition of MDM2-mediated p53 ubiquitination and degradation by ribosomal protein L5.
2004,
Pubmed Dai,
Ribosomal protein L23 activates p53 by inhibiting MDM2 function in response to ribosomal perturbation but not to translation inhibition.
2004,
Pubmed Daigle,
YjeQ, an essential, conserved, uncharacterized protein from Escherichia coli, is an unusual GTPase with circularly permuted G-motifs and marked burst kinetics.
2002,
Pubmed Du,
Yph1p, an ORC-interacting protein: potential links between cell proliferation control, DNA replication, and ribosome biogenesis.
2002,
Pubmed Du,
The homologous putative GTPases Grn1p from fission yeast and the human GNL3L are required for growth and play a role in processing of nucleolar pre-rRNA.
2006,
Pubmed Eichler,
Processing of eukaryotic ribosomal RNA.
1994,
Pubmed Fan,
Nucleostemin mRNA is expressed in both normal and malignant renal tissues.
2006,
Pubmed Francoz,
Mdm4 and Mdm2 cooperate to inhibit p53 activity in proliferating and quiescent cells in vivo.
2006,
Pubmed Han,
Cloning of the nucleostemin gene and its function in transforming human embryonic bone marrow mesenchymal stem cells into F6 tumor cells.
2005,
Pubmed Jacks,
Tumor spectrum analysis in p53-mutant mice.
1994,
Pubmed Kafienah,
Nucleostemin is a marker of proliferating stromal stem cells in adult human bone marrow.
2006,
Pubmed Korgaonkar,
Nucleophosmin (B23) targets ARF to nucleoli and inhibits its function.
2005,
Pubmed Kurki,
Nucleolar protein NPM interacts with HDM2 and protects tumor suppressor protein p53 from HDM2-mediated degradation.
2004,
Pubmed Liu,
Chk1 is an essential kinase that is regulated by Atr and required for the G(2)/M DNA damage checkpoint.
2000,
Pubmed Lohrum,
Regulation of HDM2 activity by the ribosomal protein L11.
2003,
Pubmed Migliorini,
Mdm4 (Mdmx) regulates p53-induced growth arrest and neuronal cell death during early embryonic mouse development.
2002,
Pubmed Nichols,
Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4.
1998,
Pubmed Niwa,
Phenotypic complementation establishes requirements for specific POU domain and generic transactivation function of Oct-3/4 in embryonic stem cells.
2002,
Pubmed Okamoto,
A novel octamer binding transcription factor is differentially expressed in mouse embryonic cells.
1990,
Pubmed Olson,
The nucleolus: an old factory with unexpected capabilities.
2000,
Pubmed Oren,
Decision making by p53: life, death and cancer.
2003,
Pubmed Pardee,
G1 events and regulation of cell proliferation.
1989,
Pubmed Politz,
A nonribosomal landscape in the nucleolus revealed by the stem cell protein nucleostemin.
2005,
Pubmed Racevskis,
Cloning of a novel nucleolar guanosine 5'-triphosphate binding protein autoantigen from a breast tumor.
1996,
Pubmed Reya,
Stem cells, cancer, and cancer stem cells.
2001,
Pubmed Rubbi,
Disruption of the nucleolus mediates stabilization of p53 in response to DNA damage and other stresses.
2003,
Pubmed Saka,
Spatial and temporal patterns of cell division during early Xenopus embryogenesis.
2001,
Pubmed
,
Xenbase Sasai,
Requirement of FoxD3-class signaling for neural crest determination in Xenopus.
2001,
Pubmed
,
Xenbase Saveanu,
Nog2p, a putative GTPase associated with pre-60S subunits and required for late 60S maturation steps.
2001,
Pubmed Schöler,
Oct-4: a germline-specific transcription factor mapping to the mouse t-complex.
1990,
Pubmed Strahl,
The language of covalent histone modifications.
2000,
Pubmed Strezoska,
Functional inactivation of the mouse nucleolar protein Bop1 inhibits multiple steps in pre-rRNA processing and blocks cell cycle progression.
2002,
Pubmed Sulic,
Inactivation of S6 ribosomal protein gene in T lymphocytes activates a p53-dependent checkpoint response.
2005,
Pubmed Takai,
Aberrant cell cycle checkpoint function and early embryonic death in Chk1(-/-) mice.
2000,
Pubmed Tsai,
A nucleolar mechanism controlling cell proliferation in stem cells and cancer cells.
2002,
Pubmed Tsai,
A multistep, GTP-driven mechanism controlling the dynamic cycling of nucleostemin.
2005,
Pubmed Uren,
Survivin and the inner centromere protein INCENP show similar cell-cycle localization and gene knockout phenotype.
2000,
Pubmed Weber,
Nucleolar Arf sequesters Mdm2 and activates p53.
1999,
Pubmed Wiles,
Establishment of a gene-trap sequence tag library to generate mutant mice from embryonic stem cells.
2000,
Pubmed
